Team:NUDT CHINA/Notebook
Designed Protein Degradation Method Based on
Trim21 And Nanobody -- Notebook
Jiaxin Ma ,Tianyi Zhang,Chenyu Lu and Chenxing Sun completed most of the experiments.
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Data |
Project |
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6.15-7.3 |
Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Tianyi Zhang. |
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7.4-8.3 |
Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Tianyi Zhang. |
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8.4-8.15 |
Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Tianyi Zhang. |
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8.16-8.28 |
Work on repeated validation of GFP interfering efficiency with Tianyi Zhang. |
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8.29-9.22 |
Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Tianyi Zhang. |
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9.22-10.10 |
Work on the proliferation and migration assay of cells with ErbB3 interfered with Tianyi Zhang. |
|
Data |
Project |
|
6.15-7.3 |
Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Jiaxin Ma. |
|
7.4-8.3 |
Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Jiaxin Ma. |
|
8.4-8.15 |
Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Jiaxin Ma. |
|
8.16-8.28 |
Work on repeated validation of GFP interfering efficiency with Jiaxin Ma. |
|
8.29-9.22 |
Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Jiaxin Ma. |
|
9.22-10.10 |
Work on the proliferation and migration assay of cells with ErbB3 interfered with Jiaxin Ma. |
|
Data |
Project |
|
6.15-7.3 |
Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenxing Sun. |
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7.4-8.3 |
Hela Cell culture and preparatory works on fluorescence detection with Chenxing Sun. |
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8.4-8.28 |
Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenxing Sun. |
|
8.29-9.22 |
Construct Parts with Chenxing Sun. |
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9.22-10.10 |
Work on the experimental validation of parts with Chenxing Sun. |
|
Data |
Project |
|
6.15-7.3 |
Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenyu Lu . |
|
7.4-8.3 |
Hela Cell culture and preparatory works on fluorescence detection with Chenyu Lu . |
|
8.4-8.28 |
Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenyu Lu . |
|
8.29-9.22 |
Construct Parts with Chenyu Lu . |
|
9.22-10.10 |
Work on the experimental validation of parts with Chenyu Lu . |