Team:Nanjing NFLS/Parts
Parts.
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| Part name | Short Description | Long Description |
| BBa_K2597000 | HOsite-TMCP (HO recognizable site, Cas9) | BBa_K2597000 consists of Homothallic switching endonuclease (HO) recognizable site, Mini-TK promoter, Cas9 and poly A, as we named to be HOsite-TMCP. This part codes for Cas9 gene and expresses Cas9 protein, aiming to cut the telomeres of cancer cells under the guidance of telomerase-targeting sgRNA. HO recognizable site could be recognized by HO enzyme to produce the four-base overhang (3’-TTGT-5’), which would be recognized by telomerase and lead to the synthesis of double-stranded telomeric repeat sequence. |
| BBa_K2597001 | TsgRNA-dCas9-VP64 | BBa_K259701 consists of TsgRNA designed to target telomeric repeat sequence and artificial transcription factor dCas9-VP64 to activate transcription of effector gene on the vector. We cloned the linear TsgRNA fragments target telomere into plasmid pcDNA-dCas9-VP64 to generate plasmid TsgRNA-dCas9-VP64 for convenience of using and improvement in transfection efficiency. When transfected in cells with high telomerase activity, dCas9-VP64 could bind the telomeric repeat sequence synthesized by telomerase thus activate the expression of effector gene Cas9, leading to its cutting on telomeres and the apoptosis of cancer cells. |
| BBa_K2597002 | Homothallic switching endonuclease (C1-HO) | BBa_K259702 is constructed to express HO enzyme that could cut a blunt-ended effector containing HO recognizable site (HOsite-TMCP) to produce a stick end of 3’-TTGT-5’. The stick end could be recognized by telomerase, leading to the double-stranded telomeric repeat sequence synthesis. |