Team:REC-CHENNAI/Notebook
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March 2018
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March 10, 2018
Interviews were held to select students to form the REC-CHENNAI 2018 iGEM team.
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March 16, 2018
Final team members were selected and the team was formed
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March 20, 2018
First official team meeting
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March 25, 2018
First brainstorming session
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March was the month of firsts for our team. We bounced ideas off of one another and made ourselves familiar with who we were going to work with and how we were to get as much productive work done as possible. We spent the first few meetings talking about the roles each member was going to play and how we would monitor our progress. It’s safe to say that March was the beginning of our journey into iGEM 2018.
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April 2018
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April 04, 2018
Brainstorming - 2
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April 10, 2018
Brainstorming - 3
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April 21, 2018
Brainstorming - 4
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April 25, 2018
Skype call with VIT iGEM team
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April was filled with back-to-back brainstorming sessions. With many a day spent in the deserted classrooms of our college, we managed to finalize what would be our project ‘FluoroScreen’. We familiarized ourselves with our Plan of Action and dived into the depths of Site-Directed Mutagenesis.
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May 2018
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May 08, 2018
Received the iGEM kit. Unboxing with Arun Kumar Sir.
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May 11, 2018
Lab Discussion 1
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May 17, 2018
Batch 1. Experiment: Transformation.
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May 18, 2018
Lab Discussion 2
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May 19, 2018
Skype call with NYU Abu Dhabi.
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May 29, 2018
HP team discussion
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May started with the iGEM Distribution kit on our doorstep. The entire team was there to witness it being opened along with Mr Arun Kumar our Team Advisor. We spent the rest of the month discussing our course of action and getting our lab ready for the number of hours we had to spend. We also had brainstorming sessions about what we wanted to do for Human Practices.
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June 2018
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June 06, 2018
Batch 1. Experiment: Transformation
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June 07, 2018
Batch 2. Experiment: Transformation
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June 08, 2018
Batch 3. Experiment: Transformation.
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June 11, 2018
Team meeting. Results discussion. Protocol Optimization
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June 17, 2018
Collaboration. Beach clean up with VIT Vellore and IIT-M
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June 18, 2018
Science sketch initial discussion
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June 19, 2018
Batch 1. Experiment: Transformation
WET LAB RESULTS
- Psb1c3 plasmid backbone containing BBa_J04450 ( needs to be linked) RFP construct from iGEM 2017 competent cell kit was used to perform the transformation.
- Transformation was done for 10 pg/µl and 100 pg/µl plasmid concentration after making DH5α strain of E.coli cells competent.
- After transformation, cells were plated on LB agar plates containing Chloramphenicol in two volumes 20µl and 200µl.
- Red RFP colonies were obtained and were confirmed by spectrophotometric analysis.
a) Plasmid concentration -- 10pg/µl. Cell volume plated -- 200µl
b) Plasmid concentration -- 100pg/µl. Plated cell volume -- 200µl
Experiment: Plasmid Isolation
- 1.Psb1c3 plasmid backbone containing BBa_J04450 ( needs to be in hyperlink) RFP construct from iGEM 2017 competent cell kit was used to perform the transformation.
- 2.Transformation was done for 10 pg/µl and 100 pg/µl plasmid concentration after making DH5α strain of E.coli cells competent.
- 3.After transformation, cells were plated on LB agar plates containing Chloramphenicol in two volumes 20µl and 200µl.
Red RFP colonies were obtained and were confirmed by spectrophotometric analysis
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June 20, 2018
Batch 2. Experiment: Transformation
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June 21, 2018
Batch 3. Experiment: Transformation
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June 22, 2018
Batch 1. Experiment: Transformation
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June 26, 2018
Preliminary safety forms filled. HP team discussion.
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June 27, 2018
HP team discussion.
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June 28, 2018
Batch 1. Experiment: InterLab protocol
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June 30, 2018
HP team discussion.
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June proved to the one of the busiest months for the entire team. With college functioning side-by-side, we had to push the pedal to the metal to make sure we met deadlines. With multiple optimizations and runs of transformations on-the-go, we used every spare moment we had to execute our innovative ideas for Human Practices.
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July 2018
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July 03, 2018
Batch 2. Experiment: InterLab
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July 05, 2018
HP survey forms handed out.
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July 06, 2018
Batch 1. Experiment: Transformation
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July 11, 2018
Batch 2. Experiment: InterLab protocol
WET LAB RESULTS
- 1.2018 Plate Reader and CFU protocol was performed wherein various GFP constructs were found in psb1c3 plasmid backbone.
- 2.Transformed DH5α E.coli cells were seen in plates containing the Test Device 4 with the BBa_J364007 construct.
- 3.Green GFP colonies were obtained and confirmed by spectrophotometric analysis.
a) Plasmid – psb1c3 with BBa_J364007 construct. Plated cell volume -- 200µl
b) Plasmid – psb1c3 with BBa_J364007 construct. Plated cell volume -- 200µl
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July 17, 2018
Collaboration. Beach clean up with VIT Vellore and IIT-M
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July 12, 2018
Batch 1. Experiment: Restriction Digestion Optimisation. Batch 2. Experiment: Plasmid Isolation
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July 14, 2018
Batch 3. Experiment: InterLab protocol
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July 15, 2018
Team Logo and Project Logo completed.
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July 16, 2018
HP team discussion iHP with Dr MadhavanJagadeesan
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July 17, 2018
Batch 1. Experiment: InterLab protocol
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July 18, 2018
Batch 2. Experiment: InterLab protocol
- 2018 Plate Reader and CFU protocol was performed wherein various GFP constructs were found in psb1c3 plasmid backbone.
- Transformed DH5α E.coli cells were seen in plates containing the construct present in the Negative Control ie BBa_R0040
- One green GFP colony was obtained and confirmed by spectrophotometric analysis.
a) Plasmid – psb1c3 with BBa_R0040 construct. Plated cell volume -- 200µl
Plasmid Isolation
- The protocol specified in our protocols page was followed to perform the isolation. *link*
- Furthermore, agarose gel electrophoresis was done as shown.
a) Agarose Gel Electrophoresis of isolated plasmid
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July 19, 2018
Wiki page discussion
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July 21, 2018
Batch 3. Experiment: InterLab protocol
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July 26, 2018
Batch 1. Experiment: InterLab protocol
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July 27, 2018
Batch 2. Experiment: InterLab protocol All-India iGEM meetup at IISc Bangalore
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July 31, 2018
Batch 3. Experiment: InterLab protocol
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July proved to be another busy month for us as we reached a consensus on our project name. Based on the project name, we were able to come up with a logo and a team logo that aptly explains who we are and where we come from. In the midst of many days of wet lab experiments, we had a few members of our team represent us at the All India iGEM meet that was held in IISc Bangalore.
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August 2018
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August 02, 2018
Project presentation for staff members and students.
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August 04, 2018
Skype call with our mentor MsSnehal Kadam
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August 07, 2018
Batch 1. Experiment: InterLab protocol
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August 13, 2018
HP team discussion
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August 14, 2018
Integrated HP with MrPhaneeshwar Rao from Evolva Biotech
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August 17, 2018
HP team discussion
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August 19, 2018
HP team discussion
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August 21, 2018
Final survey forms completed.
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August 27, 2018
– Posters and Banners for ‘The Hangout’ prepared. InterLab results uploaded.
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August 31, 2018
Preparation for ‘The Hangout’
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August flew past us like a blur of light and with event after event that defined what we are today. We had meetings with renowned people to figure out what we can do to make our project better. We discussed and towards the end of the month, we figured out how to execute our idea of ‘The Hangout’. Our InterLab results were uploaded thus marking the end of a journey filled with joy and happiness alike.
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September 2018
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September 02, 2018
HP Beach Meet
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September 11, 2018
Received plasmids from IDT
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September 14, 2018
Batch 1. Experiment: Transformation.
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September 15, 2018
Batch 2. Experiment: Transformation.
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September 16, 2018
Batch 3. Experiment: Transformation
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September 17, 2018
Batch 1. Experiment: Plasmid Isolation and Restriction Digestion Lab Fumigation.
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September 21, 2018
Lab Cleaning
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September 22, 2018
Batch 2.Experiment: Plasmid Isolation
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October 2018
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October 09, 2018
Batch 1. Experiment: Transformation
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October 10, 2018
Batch 2. Experiment: Transformation
Part were shipped to Boston
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October 11, 2018
Batch 3. Experiment: Transformation
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October 13, 2018
Batch 1. Experiment: Transformation