Team:Rice/Parts
Parts
| Part # | Name | Type | Description |
|---|---|---|---|
| BBa_K2540011 | Universal bacterial expression resource (UBER) | basic | 
Universal Bacterial Expression Resource (UBER) is a system containing T7 RNA Polymerase (T7 RNAP) which can be used for the expression of genes under T7 promoter. UBER includes T7 RNAP and TetR repressor which provides negative feedback on the polymerase levels to help reduce toxicity. The part was modified from Kushwaha & Salis.1 |
|
BBa_K2540010
BBa_K2540002 BBa_K2540004 BBa_K2540003 BBa_K2540001 BBa_K2540012 BBa_K2540006 BBa_K2540007 BBa_K2540009 |
Broad host range regulatory elements | basic |  Broad host range regulatory elements consist of a constitutive promoter and a ribosome binding site which can be used for gene expression across different bacterial species. The part sequences were obtained from Johns et al.2 |
| BBa_K2540015 | Orthogonal ribosome binding site | basic | 
This orthogonal RBS was designed using RBS calculator for orthogonal 16S rRNA predicted to function across a variety of bacterial strains. Prediction was done by algorithm written by our team based on previous work by Chubiz & Rao.3 |
References
[1] Kushwaha, M., & Salis, H. M. (2015). A portable expression resource for engineering cross-species genetic circuits and pathways. Nature Communications, 6(1), 7832.
[2] Johns, N. I., Gomes, A. L. C., Yim, S. S., Yang, A., Blazejewski, T., Smillie, C. S., … Wang, H. H. (2018). Metagenomic mining of regulatory elements enables programmable species-selective gene expression. Nature Methods, 15(5), 323–329.
[3] Chubiz, L. M., & Rao, C. V. (2008). Computational design of orthogonal ribosomes. Nucleic Acids Research, 36(12), 4038–4046.