All work was carried out by student members of TAS_Taipei 2018 and conducted at Taipei American School's Sandy R. Puckett Memorial Research Laboratory, unless stated otherwise. Our advisors, Jude Clapper and Teresa Chiang, helped troubleshoot our experiments and offered suggestions on all aspects of our project.
.
TEAM MEMBERS
.
Experimental
Literature Research
Whole team participated.
Cloning DNA Constructs
Catherine C, Tim H, Justin W, Leona T.
SDS-PAGE Gel
Leona T.
ALDH2 Functional Test
Justin W.
Protein Purification
Justin W, Leona T.
PalcA Ethanol Promoter Testing
Catherine C, Justin W, Jake Y.
Wiki Text
Justin W, Tim H, Catherine C, edited by Teresa Chiang and Jude Clapper
Modeling
Research
Justin L.
Program Coding
Justin L.
Experimental Data Analysis & Graphs
Justin L.
Wiki Text
Justin L, edited by Nicholas Ward, Teresa Chiang, and Jude Clapper
Prototype
E. coli Nissle 1917 Functional Test
Justin W.
Purified Enzyme Functional Test
Justin W.
E. coli Nissle 1917 & L. lactis Transformation
Catherine C, Tim H, Leona T, Justin W.
SEM Sample Prep & Imaging
Catherine C.
Ball Drop (Viscosity) Experiment
Tim H, Justin W.
E. coli Nissle 1917 Heat Threshold & Candy Experiments
Leona T, Catherine C.
Wiki Text
Justin W, Tim H, Catherine C, edited by Teresa Chiang and Jude Clapper
Human Practices
Dr. Che-Hong Chen Interview
Catherine C, Leona T, Justin Y, Justin L, Charlotte C, Longan S.
Taiwan Alcohol Intolerance Education Society (TAIES) Workshop
Emily T, Justin W, Charlotte C, Longan S, Yvonne W.
Hospital Calls
Longan S, Catherine C, Colin H, Christy C, Chloe W, Nicole C, Iris H, Caroline C, Emily T.
Bioethics Roundtable Discussion
Whole Team Activity
Survey
Emily T, Iris H, Justin L, Chloe W, Catherine C, Christy C, Nicole C, Colin H, Longan S.
Dr. Stephanie Hsieh Interview
Emily T, Tim H, Iris H, Catherine C.
Yakult Factory Visit
Catherine C, Tim H, Leona T, Justin W, Justin L.
Kavalan Distillery Visit
Emily T, Catherine C, Tim H, Justin W, Justin L.
Mrs. Michelle Bruce & Dr. Vera Wu Interview
Nicole C, Emily T, Austin H, Iris H.
Marketing Plan
Emily T, Nicole C, Austin H, Iris H.
Fundraising (Club and Spring Fair Sales)
Whole Team Activity
Alcohol Manufacturer Interview
Emily T, Iris H.
Dr. Cheng-Hua Lee Interview
Emily T.
Policy Brief
Iris H, edited by Richard Brundage
Education (7th Grade & Kindergarten)
Whole Team Activity
Class Meeting & Health Class Presentations
Catherine C, Justin W, Austin H, Catherine Y, Yvonne W, Justin Y, Emily T.
Outreach Trips (Survey and Bandage Tests)
Emily T, Justin W, Tim H, Justin L, Charlotte C, Chloe W, Iris H, Caroline C, Longan S, Catherine C.
Asia Pacific iGEM Conference
Longan S, Catherine C, Justin W, Tim H, Anna C, Yasmin L.
Collaborations
Jake Y, Anna C, Yasmin L, Catherine C.
Communication with Publications
Emily T.
Wiki Text
Emily T, Iris H, Longan S, Charlotte C, Catherine C, edited by Teresa Chiang and Jude Clapper
Wiki & Graphic Design
Wiki Coding & Visual Design
Caroline C, Charlotte C, William C.
Graphics & Animations
Caroline C.
Sticker
Paul A, Charlotte C, Emily T.
Infographic
Nicole C, Shantih W, Chloe W, Emily T.
Poster (NCTU, Giant Jamboree)
Catherine C, Tim H.
Cover Images
Justin Y, Caroline C.
PROJECT ATTRIBUTIONS
General Support
Ms. Queenie Liao, Science Department Assistant
Mr. David Iverson, Science Department Chair
Parents and friends of the TAS_Taipei 2018 team
Project Support & Advice
Dr. Che-Hong Chen
Dr. Stephanie Hsieh
Dr. Ying Chieh Tsai for recommending the use of L. lactis & E. coli Nissle 1917
Dr. Chuan-Mei Yeh for providing L. lactis
Dr. Rudolf von Bünau for providing E. coli Nissle 1917
Evelyn Lai, Kelsey Wang, and Ryan Chou for performing Interlab experiments
Phillip Wei & Ben Kao for editing the ball drop video
Guo Chen for banner design
Dylan Lin and Shin-Yi Chu for wiki support
Ethan Wu for modeling support
Fundraising Support & Advice
Dr. Dan Long & Ms. Jessica Lin
Lab Support
Integrated DNA Technologies: DNA Synthesis
MEDCLUB Scientific: IDT Distributor
Tri-I Biotech: Gene Sequencing and Primer Synthesis
THEPS Limited 中港環保工程股份有限公司: Lab Waste Treatment
Difficult Technique Support
Mr. Jude Clapper: Synthesis of acetaldehyde
Project Advisor Support
Mr. Jude Clapper
Dr. Teresa Chiang
Mr. Sean Tsao
Wiki Support
Some parts of this wiki have been derived from the 2014, 2015, 2016, 2017 TAS_Taipei wikis, which were designed entirely by the respective teams.
Presentation Coaching
Mr. Jude Clapper
Dr. Teresa Chiang
Human Practices Support
TAIES
Yakult Factory
Kavalan Distillery
Dr. Vera Wu & Mrs. Michelle Bruce
Brian Pan
The Blue & Gold
Global Views Monthly
Today’s News
ICRT Radio
The News Lens
Dr. Cheng-Hua Lee
Connie Ma for putting us in contact with ICRT and The News Lens
Ling Chen for introducing us to Dr. Cheng-Hua Lee
Collaborators
NYMU_Taipei
For providing a protocol and lending us an electroporation machine
For helping us take plate reader measurements (alcohol promoter testing)
Trained our future iGEMers (Anna C., Jake Y., Yasmin L.)
Lambert_GA
Tested their ElectroPen prototype
A huge thank you to the following individuals for supporting our research program.
DR. SHARON HENNESSY, TAS Superintendent
DR. RICHARD HARTZELL, TAS Upper School Principal
FRIENDS OF TAS
.
TEAM TRAINING & PROJECT START
Does your institution teach an iGEM or synthetic biology course? Taipei American School teaches a year long synthetic biology course. During the first semester, students learn synthetic biology techniques in both classroom and laboratory settings. During the second semester and over the summer, students work on their iGEM project.
When did you start this course? TAS started this course in 2013. The 2018 TAS_Taipei team began taking this class in August 2017.
Are the syllabus and course materials freely available online? We follow the BioBuilder curriculum (biobuilder.org).
When did you start your brainstorming? November 2017
When did you start in the lab? August 2017
When did you start working on your project? November 2017
