Team:TUST China/ Parts


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Parts

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TUST-iGEM 2018 team members are committed to the establishment of a wide range of diverse parts library. We have constructed a detection and degradation system for tetracycline and data and phenomena demonstrate that they are valid.

We use the Gibson assembly method to build our parts. This way we were able to generate our required parts in a fast and efficient way. Therefore, all of the parts we used are composite. The different basic parts comprising the composite parts are documented seperately, although they do not exist induvidually.

In the case of a constitutive expression, the promoter is included in the biobrick itself. For the inducible expression, we made use of a plasmid backbone containing a Tet-promoter ，a Lac-promoter and the LacI gene, which encodes for the Lac repressor. And we improve both the function and characterization of the some part to better express in our constructive chasis.

Name Type Description Designer Length
BBa_K2685000 Coding Lip LIN,WENXIN 1113
BBa_K2685001 Coding Mnp LIN,WENXIN 1131
BBa_K2685002 Coding TetR LIN,WENXIN 624
BBa_K2685003 Regulatory TetO LIN,WENXIN 54
BBa_K2685004 Coding TetA LIN,WENXIN 1191
BBa_K2685005 Coding φX174E LIN,WENXIN 276
BBa_K2685006 Composite TetO connected with TetA LIN,WENXIN 1245
BBa_K2685007 Composite Detecting parts LIN,WENXIN 2600
BBa_K2685008 Composite Detecting parts without TetA LIN,WENXIN 1456
BBa_K2685009 Composite Detecting parts of the GFP to change position with TetA LIN,WENXIN 2689
BBa_K2685010 Composite PLac promoter with Lip LIN,WENXIN 1248
BBa_K2685011 Composite PTet promoter with Lip and LacI LIN,WENXIN 2335
BBa_K2685012 Composite PTet promoter with Mnp and LacI LIN,WENXIN 2353
BBa_K2685013 Composite PTet promoter with Lip,Mnp and LacI LIN,WENXIN 3478
BBa_K2685014 Composite PTet promoter with Lip,Mnp,Laccase and LacI LIN,WENXIN 5032
BBa_K2685015 Composite pTet+Mnp+LacI+pLac+φX174E LIN,WENXIN 2741
BBa_K2685016 Composite pTet+Lip+LacI+pLac+φX174E LIN,WENXIN 2723
BBa_K2685017 Composite pTet+Lip+Mnp+LacI+pLac+φX174E LIN,WENXIN 3866
BBa_K2685018 Composite pTet+Lip+Mnp+Laccase+LacI+pLac+φX174E LIN,WENXIN 5420
BBa_K2685019 Reporter GFP LIN,WENXIN 717
BBa_K2685020 Regulatory J23114 LIN,WENXIN 40
BBa_K2685021 Regulatory PTet LIN,WENXIN 54
BBa_K2685022 Regulatory pLac WANG,MENG 100
BBa_K2685023 Coding LacI WANG,MENG 1144
BBa_K2685024 Coding Laccase WANG,MENG 1542
BBa_K2685025 RBS RBS WANG,MENG 12

BASIC PARTS



The different basic parts comprising the synthesized parts we used are documented here. Please note that some of these parts do not exist individually. And we improve both the function and characterization of the some part to better express in our constructive chasis.

Name Type Description Designer Length
BBa_K2685000 Coding Lip LIN,WENXIN 1113
BBa_K2685001 Coding Mnp LIN,WENXIN 1131
BBa_K2685002 Coding TetR LIN,WENXIN 624
BBa_K2685003 Regulatory TetO LIN,WENXIN 54
BBa_K2685004 Coding TetA LIN,WENXIN 1191
BBa_K2685005 Coding φX174E LIN,WENXIN 276
BBa_K2685019 Reporter GFP LIN,WENXIN 717
BBa_K2685020 Regulatory J23114 LIN,WENXIN 40
BBa_K2685021 Regulatory PTet LIN,WENXIN 54
BBa_K2685022 Regulatory pLac WANG,MENG 100
BBa_K2685023 Coding LacI WANG,MENG 1144
BBa_K2685024 Coding Laccase WANG,MENG 1542
BBa_K2685025 RBS RBS WANG,MENG 12

COMPOSITE PARTS



We use the Gibson assembly method to build our parts. This way we were able to generate our required parts in a fast and efficient way. Therefore, all of the parts we used are composite.

Name Type Description Designer Length
BBa_K2685006 Composite TetO connected with TetA LIN,WENXIN 1245
BBa_K2685007 Composite Detecting parts LIN,WENXIN 2600
BBa_K2685008 Composite Detecting parts without TetA LIN,WENXIN 1456
BBa_K2685009 Composite Detecting parts of the GFP to change position with TetA LIN,WENXIN 2689
BBa_K2685010 Composite PLac promoter with Lip LIN,WENXIN 1248
BBa_K2685011 Composite PTet promoter with Lip and LacI LIN,WENXIN 2335
BBa_K2685012 Composite PTet promoter with Mnp and LacI LIN,WENXIN 2353
BBa_K2685013 Composite PTet promoter with Lip,Mnp and LacI LIN,WENXIN 3478
BBa_K2685014 Composite PTet promoter with Lip,Mnp,Laccase and LacI LIN,WENXIN 5032
BBa_K2685016 Composite pTet+Lip+LacI+pLac+φX174E LIN,WENXIN 2723
BBa_K2685017 Composite pTet+Lip+Mnp+LacI+pLac+φX174E LIN,WENXIN 3866
BBa_K2685018 Composite pTet+Lip+Mnp+Laccase+LacI+pLac+φX174E LIN,WENXIN 5420
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