Team:William and Mary/Parts
Parts
Besides our 3G parts library, we are also submitting a series of heat inducible reporters (mScarlet) and proteases (mf-Lon), which we are using to construct IFFLs with different parameters. We have varied the promoter strength through the use of either J23107 or J23100. For a repressor we used either temperature sensitive lambda-cl which induces at 37 C, or TlpA39, which induces at 39 C. To alter degradation rate we used ptds, protein degradation tags, of varying strengths, or ssrA, another type of degradation tag.
By combining these parts in various combinations, we were able to test IFFLs of various strengths and parameters.
Heat inducible IFFLs
| K2680064 | J23107 TPL + ptlpA B0034m mScarlet-I pdt-3d thrL terminator + ptlpAr BCD8 mf-Lon ssrA |
| K2680069 | J23107 TPL + ptlpA B0034m mScarlet-I thrL terminator + ptlpAr BCD8 mf-Lon ssrA |
| K2680063 | J23107 TPL + ptlpA B0034m mScarlet-I pdt-3b thrL terminator + ptlpAr BCD8 mf-Lon ssrA |
Heat inducible protease
| K2680050 | J23107 ts-CI + R0051 mf-Lon |
| K2680057 | J23100 ts-CI + R0051 mf-Lon ssrA |
| K2680058 | J23107 TPL + ptlpA BCD8 mf-lon thrL |
| K2680059 | J23107 TPL + ptlpA BCD8 mf-lon ssrA thrL |
| K2680060 | J23107 TPL + ptlpAr BCD8 mf-lon thrL |
| K2680061 | J23107 TPL + ptlpAr BCD8 mf-lon ssrA thrL |
Heat inducible reporters
| K2680051 | J23107 ts-CI + R0051 mScarlet-I pdt#3 |
| K2680052 | J23107 ts-CI + R0051 mScarlet-I pdt#3b |
| K2680053 | J23100 ts-CI + R0051 mScarlet-I pdt#3 |
| K2680054 | J23100 ts-CI + R0051 mScarlet-I pdt#3b |
| K2680062 | J23107 TPL + ptlpA b0034m mScarlet-I pdt#3 |
| K2680065 | J23107 TPL + ptplAr B0034 mScarlet-I pdt#3 |
| K2680066 | J23107 TPL + ptplA B0034 mScarlet-I pdt#3b |
| K2680067 | J23107 TPL + ptplAr B0034 mScarlet-I pdt#3b |
| K2680068 | J23107 ts-CI + R0051 mScarlet-I |
| K2680070 | J23107 ts-CI + R0051 mScarlet-I pdt#3e |
| K2680071 | J23107 TPL + ptlpA b0034m mScarlet-I pdt#3e |