Protocols
![](https://static.igem.org/mediawiki/2018/6/65/T--William_and_Mary--Protocols_img.gif)
This page contains our standard lab protocols which are provided in a zip format containing pdfs:
In our project, we used 3G assembly which is a new and cutting edge hybrid method of DNA assembly that allows for modular assembly of multi-part circuits in a single day. In this method, circuits are composed using a combination of Golden Gate Assembly and Gibson Assembly.
For 3G assembly protocols see:
For Flow Cytometry protocols see: