Difference between revisions of "Team:NUDT CHINA/Notebook"
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Revision as of 16:24, 7 October 2018
Development of A Novel
Blood-MicroRNA Handy Detection System with CRISPR
Chuanyang Liu, Changsong Gao, Chushu Zhu and Qijie Xu completed most of the experiments.
|
Data |
Project |
|
6.15-7.4 |
Finish the culture of A549 cell and optimization of culture condition with Chuanyang Liu |
|
7.5-8.13 |
Work on Western Blot assay for target gene expression with Chuanyang Liu |
|
8.14-8.26 |
Do Western Blot assay EMT markers with Chuanyang Liu |
|
8.27-9.22 |
Finish the Cell proliferation and migration assay of miR-214 Locker group with Chuanyang Liu |
|
9.22-10.14 |
Finish the Cell proliferation and migration assay of miR-654 Locker group with Chuanyang Liu |
|
Data |
Project |
|
6.15-7.4 |
Finish the Phosphorylation and annealing of the oligos with Qijie Xu |
|
7.5-8.13 |
Work on miRNA locker assembly with Qijie Xu |
|
8.14-8.26 |
Finish the Asymmetric PCR to generate ssDNAs from dsDNAs with Qijie Xu |
|
8.27-9.22 |
Construct parts with Qijie Xu |
|
9.22-10.14 |
Work on the experimental validation of parts with Qijie Xu |
|
Data |
Project |
|
6.15-7.4 |
Finish the Phosphorylation and annealing of the oligos with Chushu Zhu |
|
7.5-8.13 |
Work on miRNA locker assembly with Chushu Zhu |
|
8.14-8.26 |
Finish the Asymmetric PCR to generate ssDNAs from dsDNAs with Chushu Zhu |
|
8.27-9.22 |
Construct parts with Chushu Zhu |
|
9.22-10.14 |
Work on the experimental validation of parts with Chushu Zhu |