Difference between revisions of "Team:NUDT CHINA/Notebook"

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<p><strong>6.15-7.4</strong></p>
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<p><strong>6.15-7.3</strong></p>
 
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<p>Finish the culture of A549 cell and optimization of culture condition&nbsp;with Chuanyang Liu</p>
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<p>Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Tianyi Zhang.</p>
 
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<p><strong>7.5-</strong><strong>8.13</strong></p>
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<p><strong>7.4-8.3</strong></p>
 
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<p>Work on Western Blot assay for target gene expression&nbsp;with Chuanyang Liu</p>
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<p>Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Tianyi Zhang.</p>
 
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<p><strong>8.</strong><strong>14-8.26</strong></p>
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<p><strong>8.4-8.15</strong></p>
 
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<p>Do Western Blot assay EMT markers&nbsp;with Chuanyang Liu</p>
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<p>Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Tianyi Zhang.</p>
 
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<p><strong>8.27-9.22</strong></p>
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<p><strong>8.16-8.28</strong></p>
 
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<p>Finish the Cell proliferation and migration assay of miR-214 Locker group&nbsp;with Chuanyang Liu</p>
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<p>Work on repeated validation of GFP interfering efficiency with Tianyi Zhang.</p>
 
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<p><strong>9.22-10.14</strong></p>
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<p><strong>8.29-9.22</strong></p>
 
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<p>Finish the Cell proliferation and migration assay of miR-654 Locker group&nbsp;with Chuanyang Liu</p>
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<p>Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Tianyi Zhang.</p>
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<p><strong>9.22-10.10</strong></p>
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<p>Work on the proliferation and migration assay of cells with ErbB3 interfered with Tianyi Zhang.</p>
 
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Revision as of 16:32, 7 October 2018

Development of A Novel

Blood-MicroRNA Handy Detection System with CRISPR

Chuanyang Liu, Changsong Gao, Chushu Zhu and Qijie Xu completed most of the experiments.

Jiaxin Ma

Data

Project

6.15-7.3

Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Tianyi Zhang.

7.4-8.3

Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Tianyi Zhang.

8.4-8.15

Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Tianyi Zhang.

8.16-8.28

Work on repeated validation of GFP interfering efficiency with Tianyi Zhang.

8.29-9.22

Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Tianyi Zhang.

9.22-10.10

Work on the proliferation and migration assay of cells with ErbB3 interfered with Tianyi Zhang.

Tianyi Zhang

Data

Project

6.15-7.4

Finish the culture of A549 cell and optimization of culture condition with Chuanyang Liu

7.5-8.13

Work on Western Blot assay for target gene expression with Chuanyang Liu

8.14-8.26

Do Western Blot assay EMT markers with Chuanyang Liu

8.27-9.22

Finish the Cell proliferation and migration assay of miR-214 Locker group with Chuanyang Liu

9.22-10.14

Finish the Cell proliferation and migration assay of miR-654 Locker group with Chuanyang Liu

Chenyu Lu

Data

Project

6.15-7.4

Finish the culture of A549 cell and optimization of culture condition with Chuanyang Liu

7.5-8.13

Work on Western Blot assay for target gene expression with Chuanyang Liu

8.14-8.26

Do Western Blot assay EMT markers with Chuanyang Liu

8.27-9.22

Finish the Cell proliferation and migration assay of miR-214 Locker group with Chuanyang Liu

9.22-10.14

Finish the Cell proliferation and migration assay of miR-654 Locker group with Chuanyang Liu

Chenxing Sun

Data

Project

6.15-7.4

Finish the culture of A549 cell and optimization of culture condition with Chuanyang Liu

7.5-8.13

Work on Western Blot assay for target gene expression with Chuanyang Liu

8.14-8.26

Do Western Blot assay EMT markers with Chuanyang Liu

8.27-9.22

Finish the Cell proliferation and migration assay of miR-214 Locker group with Chuanyang Liu

9.22-10.14

Finish the Cell proliferation and migration assay of miR-654 Locker group with Chuanyang Liu