Difference between revisions of "Team:NUDT CHINA/Notebook"

Revision as of 16:42, 7 October 2018

Development of A Novel

Blood-MicroRNA Handy Detection System with CRISPR

Chuanyang Liu, Changsong Gao, Chushu Zhu and Qijie Xu completed most of the experiments.

Jiaxin Ma
Data	Project
6.15-7.3	Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Tianyi Zhang.
7.4-8.3	Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Tianyi Zhang.
8.4-8.15	Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Tianyi Zhang.
8.16-8.28	Work on repeated validation of GFP interfering efficiency with Tianyi Zhang.
8.29-9.22	Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Tianyi Zhang.
9.22-10.10	Work on the proliferation and migration assay of cells with ErbB3 interfered with Tianyi Zhang.

Tianyi Zhang
Data	Project
6.15-7.3	Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Jiaxin Ma.
7.4-8.3	Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Jiaxin Ma.
8.4-8.15	Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Jiaxin Ma.
8.16-8.28	Work on repeated validation of GFP interfering efficiency with Jiaxin Ma.
8.29-9.22	Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Jiaxin Ma.
9.22-10.10	Work on the proliferation and migration assay of cells with ErbB3 interfered with Jiaxin Ma.

Chenyu Lu
Data	Project
6.15-7.3	Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenxing Sun.
7.4-8.3	Hela Cell culture and preparatory works on fluorescence detection with Chenxing Sun.
8.4-8.28	Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenxing Sun.
8.29-9.22	Construct Parts with Chenxing Sun.
9.22-10.10	Work on the experimental validation of parts with Chenxing Sun.

Chenxing Sun
Data	Project
6.15-7.3	Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenyu Lu .
7.4-8.3	Hela Cell culture and preparatory works on fluorescence detection with Chenyu Lu .
8.4-8.28	Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenyu Lu .
8.29-9.22	Construct Parts with Chenyu Lu .
9.22-10.10	Work on the experimental validation of parts with Chenyu Lu .

@@ Line 200: / Line 200: @@
 <tr>
 <td width="153">
-<p><strong>6.15-7.4</strong></p>
+<p><strong>6.15-7.3</strong></p>
 </td>
 <td width="538">
-<p>Finish the culture of A549 cell and optimization of culture condition&nbsp;with Chuanyang Liu</p>
+<p>Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Jiaxin Ma. </p>
 </td>
 </tr>
 <tr>
 <td width="153">
-<p><strong>7.5-</strong><strong>8.13</strong></p>
+<p><strong>7.4-8.3</strong></p>
 </td>
 <td width="538">
-<p>Work on Western Blot assay for target gene expression&nbsp;with Chuanyang Liu</p>
+<p>Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Jiaxin Ma.</p>
 </td>
 </tr>
 <tr>
 <td width="153">
-<p><strong>8.</strong><strong>14-8.26</strong></p>
+<p><strong>8.4-8.15</strong></p>
 </td>
 <td width="538">
-<p>Do Western Blot assay EMT markers&nbsp;with Chuanyang Liu</p>
+<p>Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Jiaxin Ma.</p>
 </td>
 </tr>
 <tr>
 <td width="153">
-<p><strong>8.27-9.22</strong></p>
+<p><strong>8.16-8.28</strong></p>
 </td>
 <td width="538">
-<p>Finish the Cell proliferation and migration assay of miR-214 Locker group&nbsp;with Chuanyang Liu</p>
+<p>Work on repeated validation of GFP interfering efficiency with Jiaxin Ma.</p>
 </td>
 </tr>
 <tr>
 <td width="153">
-<p><strong>9.22-10.14</strong></p>
+<p><strong>8.29-9.22</strong></p>
 </td>
 <td width="538">
-<p>Finish the Cell proliferation and migration assay of miR-654 Locker group&nbsp;with Chuanyang Liu</p>
+<p>Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Jiaxin Ma.</p>
+</td>
+</tr>
+<tr>
+<td width="153">
+<p><strong>9.22-10.10</strong></p>
+</td>
+<td width="538">
+<p>Work on the proliferation and migration assay of cells with ErbB3 interfered with Jiaxin Ma.</p>
 </td>
 </tr>
@@ Line 257: / Line 265: @@
 <tr>
 <td width="153">
-<p><strong>6.15-7.4</strong></p>
+<p><strong>6.15-7.3</strong></p>
 </td>
 <td width="538">
-<p>Finish the culture of A549 cell and optimization of culture condition&nbsp;with Chuanyang Liu</p>
+<p>Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenxing Sun.</p>
 </td>
 </tr>
 <tr>
 <td width="153">
-<p><strong>7.5-</strong><strong>8.13</strong></p>
+<p><strong>7.4-8.3</strong></p>
 </td>
 <td width="538">
-<p>Work on Western Blot assay for target gene expression&nbsp;with Chuanyang Liu</p>
+<p>Hela Cell culture and preparatory works on fluorescence detection with Chenxing Sun. </p>
 </td>
 </tr>
 <tr>
 <td width="153">
-<p><strong>8.</strong><strong>14-8.26</strong></p>
+<p><strong>8.4-8.28</strong></p>
 </td>
 <td width="538">
-<p>Do Western Blot assay EMT markers&nbsp;with Chuanyang Liu</p>
+<p>Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenxing Sun.</p>
 </td>
 </tr>
 <tr>
 <td width="153">
-<p><strong>8.27-9.22</strong></p>
+<p><strong>8.29-9.22</strong></p>
 </td>
 <td width="538">
-<p>Finish the Cell proliferation and migration assay of miR-214 Locker group&nbsp;with Chuanyang Liu</p>
+<p>Construct Parts with Chenxing Sun.</p>
 </td>
 </tr>
 <tr>
 <td width="153">
-<p><strong>9.22-10.14</strong></p>
+<p><strong>9.22-10.10</strong></p>
 </td>
 <td width="538">
-<p>Finish the Cell proliferation and migration assay of miR-654 Locker group&nbsp;with Chuanyang Liu</p>
+<p>Work on the experimental validation of parts with Chenxing Sun.</p>
 </td>
 </tr>
@@ Line 315: / Line 323: @@
 <tr>
 <td width="153">
-<p><strong>6.15-7.4</strong></p>
+<p><strong>6.15-7.3</strong></p>
 </td>
 <td width="538">
-<p>Finish the culture of A549 cell and optimization of culture condition&nbsp;with Chuanyang Liu</p>
+<p>Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenyu Lu . </p>
 </td>
 </tr>
 <tr>
 <td width="153">
-<p><strong>7.5-</strong><strong>8.13</strong></p>
+<p><strong>7.4-8.3</strong></p>
 </td>
 <td width="538">
-<p>Work on Western Blot assay for target gene expression&nbsp;with Chuanyang Liu</p>
+<p>Hela Cell culture and preparatory works on fluorescence detection with Chenyu Lu . </p>
 </td>
 </tr>
 <tr>
 <td width="153">
-<p><strong>8.</strong><strong>14-8.26</strong></p>
+<p><strong>8.4-8.28</strong></p>
 </td>
 <td width="538">
-<p>Do Western Blot assay EMT markers&nbsp;with Chuanyang Liu</p>
+<p>Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenyu Lu .</p>
 </td>
 </tr>
 <tr>
 <td width="153">
-<p><strong>8.27-9.22</strong></p>
+<p><strong>8.29-9.22</strong></p>
 </td>
 <td width="538">
-<p>Finish the Cell proliferation and migration assay of miR-214 Locker group&nbsp;with Chuanyang Liu</p>
+<p>Construct Parts with Chenyu Lu .</p>
 </td>
 </tr>
 <tr>
 <td width="153">
-<p><strong>9.22-10.14</strong></p>
+<p><strong>9.22-10.10</strong></p>
 </td>
 <td width="538">
-<p>Finish the Cell proliferation and migration assay of miR-654 Locker group&nbsp;with Chuanyang Liu</p>
+<p>Work on the experimental validation of parts with Chenyu Lu .</p>
 </td>
 </tr>