Difference between revisions of "Team:BJRS China/InterLab"
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Revision as of 06:55, 12 October 2018
InterLab
Preparation
What Is Interlab?
This is the 5th year of InterLab and we have the following question: Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?
Materials
DNA/Plasmids
- Negative Control: BBa_R0040
- Positive Control: BBa_I20270
- Test Device 1: BBa_J364000
- Test Device 2: BBa_J364001
- Test Device 3: BBa_J364002
- Test Device 4: BBa_J364007
- Test Device 5: BBa_J364008
- Test Device 6: BBa_J364009
Apparatus
- 96 well plates (provided by Peking University)
- Plate reader
- Foil covered 50 ml tube
- Eppendorf tubes
- Pipettes
Materials
- LUDOX CL-X
- Silica beads
- Fluorescein
- Phosphate buffered saline
- LB media
- Chloramphenicol
- LB plates
- distilled water
Protocols
We followed the protocol provided by iGEM HQ so that inter-laboratory errors can be reduced. Protocols we used can be found here:
2018 InterLab Plate Reader ProtocolHelp: Protocols/Transformation
Experiment Process
Results
The results are shown in the tables and figures.
I.Calibrations
OD600 reference point:
Table 1. OD600 Reference Point.
Particle Standard Curve
Figure 1. Particle Standard Curve.
Particle Standard Curve(log scale)
Figure 1. Particle Standard Curve(log scale).
Fluorescein Standard Curve
Figure 2. Fluorescein Standard Curve.
Fluorescein Standard Curve(log scale)
Figure 2. Fluorescein Standard Curve(log scale).
II.Cell Measurements
Fluorescence Raw
Table 2. Raw Plate Reader Measurements of Fluorescence Raw at 0 hour.
Table 3. Raw Plate Reader Measurements of Fluorescence Raw at 6 hours.
Abs600 Raw
Table 4. Raw Plate Reader Measurements of Abs600 Raw at 0 hour.
Table 5. Raw Plate Reader Measurements of Abs600 Raw at 6 hours.