Difference between revisions of "Team:Linkoping Sweden/Improve"

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With the use of site-direted mutagenesis we wanted to improve the part of last years LiU iGEM team. This was done through removing the fusion protein and the unnecessary bases as shown in fig. 1. By doing this the fluorescent protein mNeonGreen (mNG) can be used as a reporter in a fully functional operone, with the use of the AraC/pBAD inducible system.  
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With the use of site-direted mutagenesis we wanted to improve the part of last years LiU iGEM team. This was done through removing the fusion protein and the unnecessary bases as shown in figure below. By doing this the fluorescent protein mNeonGreen (mNG) can be used as a reporter in a fully functional operone, with the use of the AraC/pBAD inducible system.  
 
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<img src="https://static.igem.org/mediawiki/2018/d/d1/T--Linkoping_Sweden--improve.jpg"></img>
 
<img src="https://static.igem.org/mediawiki/2018/d/d1/T--Linkoping_Sweden--improve.jpg"></img>
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<h4> Figure 1. Improve. </h4>
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Revision as of 13:32, 14 October 2018

LiU iGEM

Improve

Improvement of Liu iGEM 2017s part

BBa_K2474000

With the use of site-direted mutagenesis we wanted to improve the part of last years LiU iGEM team. This was done through removing the fusion protein and the unnecessary bases as shown in figure below. By doing this the fluorescent protein mNeonGreen (mNG) can be used as a reporter in a fully functional operone, with the use of the AraC/pBAD inducible system.