Difference between revisions of "Team:BJRS China/Improve"
| Line 115: | Line 115: | ||
<figure class="text-center"> | <figure class="text-center"> | ||
| − | <img src="https://static.igem.org/mediawiki/parts/b/b5/T--BJRS_China--improvement009.png" width="50%" > | + | <img src="https://static.igem.org/mediawiki/parts/b/b5/T--BJRS_China--improvement009.png"width="50%" > |
<figcaption><div style="padding-left:300px;padding-right:300px"><b>Figure 1</b> the framework of BBa_K2833009. | <figcaption><div style="padding-left:300px;padding-right:300px"><b>Figure 1</b> the framework of BBa_K2833009. | ||
</div> </figcaption> | </div> </figcaption> | ||
</figure> | </figure> | ||
Revision as of 16:45, 14 October 2018
Improvement
Design
Our project used surface display system, and we choose the INP-YFP expressiong part BBa_K523013 built by Edinburgh 2011 to construct the INP-mediated surface display for our interest protein. While testing the surface display efficiency of this part, we found that the fluorescence intensity was too weak both under the uv-light（adjusted to the same OD600）(Fig.2, middle) and under the super resolution microscope(Fig.3, middle).
To characterize the surface display efficiency of INP better, we added a strong promoter BBa_J23104 and RBS BBa_B0032 to the upstream of INP, and changed the YFP to sfGFP, to construct our parts BBa_K2833009(Fig.1)
<figure class="text-center">
<img src="<figcaption>"width="50%" >
Figure 1 the framework of BBa_K2833009.
</figcaption>
</figure>