Difference between revisions of "Team:BJRS China/Improve"
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<h2>Design</h2> | <h2>Design</h2> | ||
| − | <p><font size="4">Our project used surface display system, and we choose the INP-YFP expressiong part <html><a href='http://parts.igem.org/Part:BBa_K523013'>BBa_K523013</a></html> built by Edinburgh 2011 to construct the INP-mediated surface display for our interest protein. While testing the surface display efficiency of this part, we found that the fluorescence intensity was too weak both under the uv-light（adjusted to the same OD<sub>600</sub>）(Fig.2, middle) and under the super resolution microscope(Fig.3, middle). | + | <p><font size="4">Our project used surface display system, and we choose the INP-YFP expressiong part <html><a href='http://parts.igem.org/Part:BBa_K523013'>BBa_K523013</a></html> built by Edinburgh 2011 to construct the INP-mediated surface display for our interest protein. While testing the surface display efficiency of this part, we found that the fluorescence intensity was too weak both under the uv-light（adjusted to the same OD<sub>600</sub>）(Fig.2, middle) and under the super resolution microscope(Fig.3, middle).</p> |
<p><font size="4">To characterize the surface display efficiency of INP better, we added a strong promoter <html><a href='http://parts.igem.org/Part:BBa_J23104'>BBa_J23104</a></html> and RBS <html><a href='http://parts.igem.org/Part:BBa_B0032'>BBa_B0032</a></html> to the upstream of INP, and changed the YFP to sfGFP, to construct our parts <html><a href='http://parts.igem.org/Part:BBa_K2833009'>BBa_K2833009</a></html>(Fig.1)</font></p> | <p><font size="4">To characterize the surface display efficiency of INP better, we added a strong promoter <html><a href='http://parts.igem.org/Part:BBa_J23104'>BBa_J23104</a></html> and RBS <html><a href='http://parts.igem.org/Part:BBa_B0032'>BBa_B0032</a></html> to the upstream of INP, and changed the YFP to sfGFP, to construct our parts <html><a href='http://parts.igem.org/Part:BBa_K2833009'>BBa_K2833009</a></html>(Fig.1)</font></p> | ||
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</div> </figcaption> | </div> </figcaption> | ||
</figure> | </figure> | ||
| + | </div> | ||
| + | </html> | ||
Revision as of 16:46, 14 October 2018
Improvement
Design
Our project used surface display system, and we choose the INP-YFP expressiong part BBa_K523013 built by Edinburgh 2011 to construct the INP-mediated surface display for our interest protein. While testing the surface display efficiency of this part, we found that the fluorescence intensity was too weak both under the uv-light（adjusted to the same OD600）(Fig.2, middle) and under the super resolution microscope(Fig.3, middle).</p>
To characterize the surface display efficiency of INP better, we added a strong promoter BBa_J23104 and RBS BBa_B0032 to the upstream of INP, and changed the YFP to sfGFP, to construct our parts BBa_K2833009(Fig.1)
<figure class="text-center">
<img src="<figcaption>"width="50%" >
Figure 1 the framework of BBa_K2833009.
</figcaption>
</figure>
</div> </html>