Difference between revisions of "Team:BJRS China/Improve"

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<h2>Results</h2>
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    <p><font size = "4">The overnight cultured bacteria expressing GFP, INP-YFP and INP-GFP respectively were firstly adjusted to OD<sub>600</sub> = 1.0 to assure the same cell counts, then 1 mL bacteria solution of each was centrifuged to get the sediment of whole cell. From the fluorescence strength of the of whole cell(<I>E.coli</I>, DH5α) precipitation under UV-light we can see that the BBa_K523013 transformed cells showed the weakest fluorescence, suggesting that our new construct can give out stronger fluorescence.</p>
 
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Revision as of 17:02, 14 October 2018

BJRS


Improvement

Design

Our project used surface display system, and we choose the INP-YFP expressiong part BBa_K523013 built by Edinburgh 2011 to construct the INP-mediated surface display for our interest protein. While testing the surface display efficiency of this part, we found that the fluorescence intensity was too weak both under the uv-light（adjusted to the same OD600）(Fig.2, middle) and under the super resolution microscope(Fig.3, middle).</p>

To characterize the surface display efficiency of INP better, we added a strong promoter BBa_J23104 and RBS BBa_B0032 to the upstream of INP, and changed the YFP to sfGFP, to construct our parts BBa_K2833009(Fig.1)

<figure class="text-center"> 

               <img src="T--BJRS_China--improvement009.png"width="50%" >
<figcaption>
Figure 1 the framework of BBa_K2833009.
</figcaption> 
           </figure>

Results

The overnight cultured bacteria expressing GFP, INP-YFP and INP-GFP respectively were firstly adjusted to OD600 = 1.0 to assure the same cell counts, then 1 mL bacteria solution of each was centrifuged to get the sediment of whole cell. From the fluorescence strength of the of whole cell(E.coli, DH5α) precipitation under UV-light we can see that the BBa_K523013 transformed cells showed the weakest fluorescence, suggesting that our new construct can give out stronger fluorescence.</p> </div> </html>