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<h2>Design</h2> | <h2>Design</h2> | ||
− | <p><font size="4">Our project used surface display system, and we choose the INP-YFP expressiong part | + | <p><font size="4">Our project used surface display system, and we choose the INP-YFP expressiong part <a href='http://parts.igem.org/Part:BBa_K523013'>BBa_K523013</a> built by Edinburgh 2011 to construct the INP-mediated surface display for our interest protein. While testing the surface display efficiency of this part, we found that the fluorescence intensity was too weak both under the uv-light(adjusted to the same OD<sub>600</sub>)(Fig.2, middle) and under the super resolution microscope(Fig.3, middle).</p> |
− | <p><font size="4">To characterize the surface display efficiency of INP better, we added a strong promoter | + | <p><font size="4">To characterize the surface display efficiency of INP better, we added a strong promoter <a href='http://parts.igem.org/Part:BBa_J23104'>BBa_J23104</a> and RBS <a href='http://parts.igem.org/Part:BBa_B0032'>BBa_B0032</a> to the upstream of INP, and changed the YFP to sfGFP, to construct our parts <a href='http://parts.igem.org/Part:BBa_K2833009'>BBa_K2833009</a>(Fig.1)</font></p> |
− | + | <figure class="text-center"> | |
− | <figure | + | |
<img src="https://static.igem.org/mediawiki/parts/b/b5/T--BJRS_China--improvement009.png"width="50%" > | <img src="https://static.igem.org/mediawiki/parts/b/b5/T--BJRS_China--improvement009.png"width="50%" > | ||
<figcaption><div style="padding-left:300px;padding-right:300px"><b>Figure 1</b> the framework of BBa_K2833009. | <figcaption><div style="padding-left:300px;padding-right:300px"><b>Figure 1</b> the framework of BBa_K2833009. |
Revision as of 02:32, 15 October 2018