This year team William and Mary's project focused on measuring the dynamical outputs of our decoding circuit. In the process we performed single cell time series measurements and developed novel protocols for the removal of small molecule inducers. Although single cell measurements using flow cytometry are our preferred way to obtain data, as our project developed, it became clear that much higher throughput would be required. As such, we spent a major portion of our project creating methods to test qualitative circuit behavior using a plate reader. However