Difference between revisions of "Team:Hong Kong HKUST"

 
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<img src="https://static.igem.org/mediawiki/2018/e/e1/T--Hong_Kong_HKUST--_LAB-1.jpeg" alt="" />
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<p>We degrade polyethylene and generate electricity from it! <br> <a href="https://2018.igem.org/Team:Hong_Kong_HKUST/Description">CLICK HERE FOR MORE</a> </br> </p>
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<h2>PEkaC<sub>2</sub>H<sub>4</sub>U</h2>
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<p>Engineer <i>E. coli</i> with Laccase gene to degrade PE into smaller alkane chains</p>
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<h2>Polyethylene Degradation</h2>
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<p>Metabolise alkane chains to convert it into <i>Shewanella oneidensis</i> food source</p>
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<h2>Alkane Metabolism</h2>
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<p>Use <i>Shewanella oneidensis</i> inbuilt <br>extracellular electron transport mechanism to produce electricity</p>
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<h2>The Microbial Fuel Cell</h2>
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<h2><a href ="https://2018.igem.org/Team:Hong_Kong_HKUST/Human_Practices"> Human Practices </a></h2>
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    <h3 style="color:grey;padding-bottom:-2em">IGEM 2018 - TEAM HKUST</h3>
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    <h2>PROJECT &nbsp;OVERVIEW</h2>
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<p style="color:black;">
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The iGEM HKUST 2018 team attempts to generate electricity from the degradation of the most widely used plastic, polyethylene (PE), using a synthetic biology approach. Making use of <i>E. coli</i> engineered with genes encoding for laccase to degrade polyethylene into smaller alkane chains, our team recognizes the opportunity to further advance this project by addressing another key issue – energy. Using <i>Shewanella oneidensis</i> MR-1 strain’s inbuilt extracellular electron transport mechanism in tandem with genes responsible for alkane metabolism derived from <i>Desulfatibacillum alkenivorans</i>, we will generate electricity from the metabolism of degraded polyethylene, hoping that it will one day help in solving the world’s growing energy needs. Thus, our project serves as an integrated effort to simultaneously solve two crucial problems.
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  <h3 style="color:grey;padding-bottom:-2em">IGEM 2018 - TEAM HKUST</h3>
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        <h2>MFC DESIGN</h2>
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  <p style="color:black;"> Aside from plastic degradation and alkane metabolism, generation of electricity was another important focus of our iGEM project. </p>
  
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<p style="color:black;">For the Microbial Fuel Cell design, we focused on generating a stable electrical current by utilizing <i>Shewanella oneidensis MR-1</i> strain’s inbuilt extracellular electron transport mechanism. In order to better harness its electrogenicity, we housed a culture of the bacterium within a microbial fuel cell of our design, aiming at maximizing electrical output for a given amount of substrate.
  
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<h3>Project Description</h3>
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<p style="color:black;">Our final design of biosphere-MFC conjugation had integrated the different comments and suggestions from our potential users. This MFC design aimed to be used both in households or indoor public areas. </p>
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<p>Polyethylene is the most widely used plastic and arguably one of the most versatile materials to ever be synthesized. Its practicality and convenience however, have come at a great environmental cost. Polyethylene takes millennia to decompose, leeching harmful microplastics into the environment. The iGEM HKUST 2018 team approaches this pressing issue from a synthetic biology perspective, making use of transformant E. coli engineered with genes encoding for Laccase to degrade polyethylene into smaller alkane chains. Our team recognizes the opportunity to further advance this project by addressing another key issue – energy. Using <i>Shewanella oneidensis</i> MR-1 strain’s inbuilt extracellular electron transport mechanism in tandem with genes responsible for alkane metabolism derived from <i>Desulfatibacillum alkenivorans</i>, we generate electricity from the metabolism of degraded polyethylene, hoping that it will one day solve the world’s growing energy needs. Thus, our project serves as an integrated effort to simultaneously solve two crucial problems.</p>
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<h1> Welcome to iGEM 2018! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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            <a href="https://2018.igem.org/Team:Hong_Kong_HKUST/Design"><button type="button" >Learn More</button></a>
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<h3 style="color:grey;padding-bottom:-2em">IGEM 2018 - TEAM HKUST</h3>
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<h2>MODELLING</h2>
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<p style="color:black"> Modeling plays a huge role in the whole project, as we fill in the gap between the experimental results and the existing data.</p>
  
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<p style="color:black">We characterized PE degradation rate based on previous iGEM data and predict how much adding OmpA to the sequence is going to affect the result of the laccase secretion. Kinetic parameters of fumarate addition mechanism were attempted to observe the activity of ASS genes and monitor the rate of conversion from alkane to succinate. This is very crucial since we do not have any laboratory experiment result to compare with. At the same time, we adopt Flux Balance Analysis to characterize how different factors from culturing media can affect direct electron transfers. For the MFC module, we have successfully established data to find the optimum culturing medium concentration for <i>Shewanella oneidensis MR-1</i> growth, which aids the design of MFC experiment. Voltage and power density that can be produced from alkane were also estimated.</p>
  
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<p style="color:black;">The innovative part of our modeling is using the existing model to further explore the effect of fumarate on the integrated alkane metabolism system, which guided our experimental design and will hopefully inspire future work on similar systems.</p>
  
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        <h3 style="color:grey;padding-bottom:-2em">IGEM 2018 - TEAM HKUST</h3>
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  <h2> HUMAN PRACTICES</h2>
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Public education is presented in the form of exhibition during our university's information day (open day) with the following goals:</p>
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<p style="color:black;">1. Promote synthetic biology as a tool to solve current problems and explain how the synthetic biology field tries to involve safety, ethics, policies, and the environment into our research and product designs. </p>
  
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<h3>Before you start</h3>
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<p style="color:black;">2. Promote renewable energies as a part of our project theme of environmental sustainability.</p>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<p style="color:black;">3. Integrate stakeholders to our product design by demonstrating our project and conducting surveys from our potential users to discover public’s concerns and area of focus for product improvements.
  
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<p style="color:black"> The survey data from our public engagement exhibition, collaborations, and interviews with Prof. Davis Bookhart (department head of the HKUST sustainability office), had been integrated into the systematic design of our Microbial Fuel Cell. </p>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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{{ Hong_Kong_HKUST/footer }}

Latest revision as of 21:53, 15 October 2018

iGem HKUST 2018

IGEM 2018 - TEAM HKUST

PROJECT  OVERVIEW

The iGEM HKUST 2018 team attempts to generate electricity from the degradation of the most widely used plastic, polyethylene (PE), using a synthetic biology approach. Making use of E. coli engineered with genes encoding for laccase to degrade polyethylene into smaller alkane chains, our team recognizes the opportunity to further advance this project by addressing another key issue – energy. Using Shewanella oneidensis MR-1 strain’s inbuilt extracellular electron transport mechanism in tandem with genes responsible for alkane metabolism derived from Desulfatibacillum alkenivorans, we will generate electricity from the metabolism of degraded polyethylene, hoping that it will one day help in solving the world’s growing energy needs. Thus, our project serves as an integrated effort to simultaneously solve two crucial problems.

IGEM 2018 - TEAM HKUST

MFC DESIGN

Aside from plastic degradation and alkane metabolism, generation of electricity was another important focus of our iGEM project.

For the Microbial Fuel Cell design, we focused on generating a stable electrical current by utilizing Shewanella oneidensis MR-1 strain’s inbuilt extracellular electron transport mechanism. In order to better harness its electrogenicity, we housed a culture of the bacterium within a microbial fuel cell of our design, aiming at maximizing electrical output for a given amount of substrate.

Our final design of biosphere-MFC conjugation had integrated the different comments and suggestions from our potential users. This MFC design aimed to be used both in households or indoor public areas.

IGEM 2018 - TEAM HKUST

MODELLING

Modeling plays a huge role in the whole project, as we fill in the gap between the experimental results and the existing data.

We characterized PE degradation rate based on previous iGEM data and predict how much adding OmpA to the sequence is going to affect the result of the laccase secretion. Kinetic parameters of fumarate addition mechanism were attempted to observe the activity of ASS genes and monitor the rate of conversion from alkane to succinate. This is very crucial since we do not have any laboratory experiment result to compare with. At the same time, we adopt Flux Balance Analysis to characterize how different factors from culturing media can affect direct electron transfers. For the MFC module, we have successfully established data to find the optimum culturing medium concentration for Shewanella oneidensis MR-1 growth, which aids the design of MFC experiment. Voltage and power density that can be produced from alkane were also estimated.

The innovative part of our modeling is using the existing model to further explore the effect of fumarate on the integrated alkane metabolism system, which guided our experimental design and will hopefully inspire future work on similar systems.

IGEM 2018 - TEAM HKUST

HUMAN PRACTICES

Public education is presented in the form of exhibition during our university's information day (open day) with the following goals:

1. Promote synthetic biology as a tool to solve current problems and explain how the synthetic biology field tries to involve safety, ethics, policies, and the environment into our research and product designs.

2. Promote renewable energies as a part of our project theme of environmental sustainability.

3. Integrate stakeholders to our product design by demonstrating our project and conducting surveys from our potential users to discover public’s concerns and area of focus for product improvements.

The survey data from our public engagement exhibition, collaborations, and interviews with Prof. Davis Bookhart (department head of the HKUST sustainability office), had been integrated into the systematic design of our Microbial Fuel Cell.

©IGEM HKUST 2018. All rights reserved.