Difference between revisions of "Team:Imperial College/Mechanisms"

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<h3>Design Overview</h3>
 
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<p2>PixCell is a major improvement on an existing electrogenetic system, making it aerobic, more responsive and fully modular. The system depends on a complex network of electrochemical, chemical and biomolecular interactions which is not yet fully understood. Chemical species and the cellular environment is maintained in a reduced form. Electrode potentials can either maintain this state or oxidise chemical species to activate the biological circuit. </p2>
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<p2>PixCell is a major improvement on an existing electrogenetic system, making it aerobic, more responsive and fully modular. The system depends on a complex network of electrochemical, chemical and biomolecular interactions.</p2>
  
 
<h3>Electrochemical Module Design</h3>
 
<h3>Electrochemical Module Design</h3>
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                 <p2>Redox molecules are maintained in a reduced form in ambient conditions. Application of a +0.5V potential oxidises the redox molecules, allowing for activation of the genetic circuit. Application of a -0.3V potential ensures the redox molecules remain reduced, preventing activation of the genetic circuit.</p2>
 
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                     <h4>Potentiostat:</h4>
 
                     <h4>Potentiostat:</h4>
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<p2>The controller of the systen. It generates the electrode currents.</p2>
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<p2>The controller of the system. It generates either a +0.5V (ON) or -0.3V (OFF) current in the electrodes.</p2>
  
 
<h4>Electrode or Electrode Array:</h4>
 
<h4>Electrode or Electrode Array:</h4>

Revision as of 22:42, 15 October 2018


Design

Design Overview

PixCell is a major improvement on an existing electrogenetic system, making it aerobic, more responsive and fully modular. The system depends on a complex network of electrochemical, chemical and biomolecular interactions.

Electrochemical Module Design

Redox molecules are maintained in a reduced form in ambient conditions. Application of a +0.5V potential oxidises the redox molecules, allowing for activation of the genetic circuit. Application of a -0.3V potential ensures the redox molecules remain reduced, preventing activation of the genetic circuit.

Potentiostat:

The controller of the system. It generates either a +0.5V (ON) or -0.3V (OFF) current in the electrodes.

Electrode or Electrode Array:

The electrode is the driving force of the system. It allows for oxidation and reduction of redox molecules.

Pyocyanin:

This is a redox-cycling molecule produced by Pseudomonas aeuriginosa. In its oxidised form it can activate gene transcription of redox-signalling pathways, either directly or by oxidative stress. In normal aerobic conditions it is oxidised.

Ferrocyanide/Ferricyanide:

These molecules are well known redox mediators. The oxidised form (ferrocyanide) can draw electrons from the cells quinone pool. This amplifies the activity of redox-cycling molecules like pyocyanin. When the reduced form (ferricyanide) is present the opposite is true.

Sodium Sulfite:

This is an oxygen scavenger as it removes oxygen from solution. This allows for pyocyanin to be maintained in a reduced form but does not diminish GFP fluorescence, suggesting cells remain in aerobic conditions.