Difference between revisions of "Team:Imperial College/IHP"
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A cursory look at the costs of PMS, pyocyanin and common inducer molecules (such as IPTG) already reveal stark differences in costs per gram. When accounting for working concentrations, this difference is further magnified, with PMS being 268 or 2680 times cheaper than IPTG (depending on levels of IPTG used described by a NEB protocol provided here:https://www.neb.com/protocols/1/01/01/protein-expression-using-bl21de3-c2527) and 3584 times cheaper than pyocyanin. These costs are summarized in a table below. However costs only matter if it can be shown that PMS can have a similar fold induction to common inducer molecules such as IPTG and experimental results for fold induction suggesting that is the case can be found below.</br> | A cursory look at the costs of PMS, pyocyanin and common inducer molecules (such as IPTG) already reveal stark differences in costs per gram. When accounting for working concentrations, this difference is further magnified, with PMS being 268 or 2680 times cheaper than IPTG (depending on levels of IPTG used described by a NEB protocol provided here:https://www.neb.com/protocols/1/01/01/protein-expression-using-bl21de3-c2527) and 3584 times cheaper than pyocyanin. These costs are summarized in a table below. However costs only matter if it can be shown that PMS can have a similar fold induction to common inducer molecules such as IPTG and experimental results for fold induction suggesting that is the case can be found below.</br> | ||
<b>Placeholder for table</b> | <b>Placeholder for table</b> | ||
| − | </br></br><a class="btn btn | + | </br></br><a class="btn btn-info" href="https://2018.igem.org/Team:Imperial_College/Demonstrate#expt7" role="button">Click here for experimental results</a></br> |
</p1> | </p1> | ||
Revision as of 03:35, 16 October 2018
Integrated HP
Summary of Integrated Human Practices
Safety
Toxicity comparison between Pyocyanin and PMS
The 2012 OSHA Hazard Communication Standard ranks hazard ratings with the use of categories, with Category 0 being the lowest risk and Category 4 being the highest. With regards to toxicity, pyocyanin is a Category 4 substance and extreme care was taken during our wet lab to ensure our own safety and any contact with pyocyanin would warrant immediate medical attention, PMS on the other hand is a Category 0 substance and thus is far easier and safer to handle. OSHA pages on PMS (http://datasheets.scbt.com/sc-215700.pdf): OSHA pages on Pyocyanin (http://datasheets.scbt.com/sds/aghs/en/sc-205475.pdf).Cost comparison between PMS and common inducer molecules
A cursory look at the costs of PMS, pyocyanin and common inducer molecules (such as IPTG) already reveal stark differences in costs per gram. When accounting for working concentrations, this difference is further magnified, with PMS being 268 or 2680 times cheaper than IPTG (depending on levels of IPTG used described by a NEB protocol provided here:https://www.neb.com/protocols/1/01/01/protein-expression-using-bl21de3-c2527) and 3584 times cheaper than pyocyanin. These costs are summarized in a table below. However costs only matter if it can be shown that PMS can have a similar fold induction to common inducer molecules such as IPTG and experimental results for fold induction suggesting that is the case can be found below. Placeholder for table Click here for experimental results
Environment
Biocontainment
Placeholder for Biocontainment GIF(http://www.loc.gov/law/help/restrictions-on-gmos/eu.php)
. By controlling transcription of growth retardants or toxins, like gp2 and MazF respectively, we can control where our bacteria will live and thus add a layer of biocontainment. Click here for experimental resultsFabric Bioprinter
Placeholder for Fabric Bioprinter GIFWellbeing
