Difference between revisions of "Team:Tokyo Tech/Parts"
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| − | + | Best Parts: EGFP-FMDV2a (<a href="parts.igem.org/Part:BBa_K2729006">BBa_K2729006</a>) | |
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| − | + | Our team made new pseudovirus that can infect Vero cells and introduce the gene including this EGFP-FMDV2a. With the assistance of "self-cleaving" 2A peptides, fluorescence protein is produced inside of the cell and folded so that it can emit fluorescence at the end. Since EGFP contains chromophore mutations that make the protein 35 times brighter than wild-type GFP | |
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| + | Since EGFP is 35 times brighter than wild-type GFP and FMDV2a precisely works in our assumption, you can easily check the degree of infection by measuring fluorescence intensity. | ||
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Revision as of 15:16, 16 October 2018
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Basic Parts
Article header with background image and parallax effect
Basic Parts
TokyoTech 2018 iGEM Team Basic Parts
| Name | Type | Description | Design | Length(bp) |
|---|---|---|---|---|
| BBa_K2729000 | Coding | DENV1 C | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 300 |
| Coding | DENV2 C | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 300 | |
| BBa_K2729002 | Coding | DENV3 C | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 300 |
| BBa_K2729003 | Coding | DENV4 C | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 300 |
| BBa_K2729004 | Coding | DENV3 E | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 1479 |
| BBa_K2729005 | Coding | DENV4 E | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 1485 |
| BBa_K2729006 | Coding | EGFP_FMDV2a | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 948 |
| BBa_K2729007 | Coding | DsRed_FMDV2a | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 908 |
| BBa_K2729008 | Coding | YFP_FMDV2a | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 945 |
| BBa_K2729009 | Coding | AmCyan_FMDV2a | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 918 |
| BBa_K2729010 | Coding | DENV1 AncC_prM_E | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 2028 |
| BBa_K2729011 | Coding | DENV2 AncC_prM_E | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 2028 |
| BBa_K2729012 | Coding | DENV3 AncC_prM_E | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 2022 |
| BBa_K2729013 | Coding | DENV4 AncC_prM_E | Hayato Ito, Kotaro Miyamoto, Hajime Fujita | 2028 |
