Difference between revisions of "Team:William and Mary/Notebook"

Line 46: Line 46:
 
<div>
 
<div>
 
   
 
   
 
+
<div style='padding-top: 40px;'></div>
  
 
<div style = 'padding-left: 8%; padding-bottom: 10px;font-size: 25px' ><b> Week 2: 180604-180610</b></div>
 
<div style = 'padding-left: 8%; padding-bottom: 10px;font-size: 25px' ><b> Week 2: 180604-180610</b></div>

Revision as of 16:34, 16 October 2018

Notebook

Week 1: 180528-180604
  • Began cloning 3G parts based off the library sent from the Murray lab
  • Researched literature on dual inducible promoters
  • Sent out several cloned 3G parts for sequencing
  • Planned out several IFFLs to test.
Week 2: 180604-180610
  • Continued cloning 3G parts onto our standard WM Pad backbone
  • Redesigned primers for cloning 3G promoter parts, as previous ones were faulty
  • Received results from sequencing, confirmed parts
  • Beginning to assemble circuits using 3G assembly
  • Making inducible reporter construct and an mf-Lon based IFFL