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<h3>Wellbeing</h3> | <h3>Wellbeing</h3> | ||
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− | <p1>When we started our project, many of us had personal as well as interpersonal issues that threatened the viability of our project as well as our own well-being. We made it a point to reflect upon this experience and wondered if any other teams had similar issues to us. We surveyed 67 people from 13 different iGEM teams and developed a team-communication application called Let's Talk about It! as a aid for resolving these issues both for us and future iGEM teams. Most importantly, we have used the app to raise important issues with our PIs and supervisors and have these issues responded to as fast as possible. Knowing that this tool exists has made us more open about our issues and help each other communicate solutions for these issues. This has raised our productivity and made us more cooperative. More information on our Team-communication application can be found <b>here</b></p1> | + | <p1>When we started our project, many of us had personal as well as interpersonal issues that threatened the viability of our project as well as our own well-being. We made it a point to reflect upon this experience and wondered if any other teams had similar issues to us. We surveyed 67 people from 13 different iGEM teams and developed a team-communication application called Let's Talk about It! as a aid for resolving these issues both for us and future iGEM teams. Most importantly, we have used the app to raise important issues with our PIs and supervisors and have these issues responded to as fast as possible. Knowing that this tool exists has made us more open about our issues and help each other communicate solutions for these issues. This has raised our productivity and made us more cooperative. More information on our Team-communication application can be found <a href="https://2018.igem.org/Team:Imperial_College/ltat"><b>here</b></a></p1> |
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<script> | <script> |
Revision as of 22:52, 16 October 2018
Integrated HP
![](https://static.igem.org/mediawiki/2018/7/77/T--Imperial_College---IHP.png)
Summary of Integrated Human Practices
Safety
Toxicity comparison between Pyocyanin and PMS
The 2012 OSHA Hazard Communication Standard ranks hazard ratings with the use of categories, with Category 0 being the lowest risk and Category 4 being the highest. With regards to toxicity, pyocyanin is a Category 4 substance and extreme care was taken during our wet lab to ensure our own safety and any contact with pyocyanin would warrant immediate medical attention, PMS on the other hand is a Category 0 substance and thus is far easier and safer to handle. OSHA pages on PMS (http://datasheets.scbt.com/sc-215700.pdf): OSHA pages on Pyocyanin (http://datasheets.scbt.com/sds/aghs/en/sc-205475.pdf).Cost comparison between PMS and common inducer molecules
A cursory look at the costs of PMS, pyocyanin and common inducer molecules (such as IPTG) already reveal stark differences in costs per gram. When accounting for working concentrations, this difference is further magnified, with PMS being 268 or 2680 times cheaper than IPTG (depending on levels of IPTG used described by a NEB protocol provided here:https://www.neb.com/protocols/1/01/01/protein-expression-using-bl21de3-c2527) and 3584 times cheaper than pyocyanin. These costs are summarized in a table below. However costs only matter if it can be shown that PMS can have a similar fold induction to common inducer molecules such as IPTG and experimental results for fold induction suggesting that is the case can be found below.![](https://static.igem.org/mediawiki/2018/8/8a/T--Imperial_College--costtable.png)
![](https://static.igem.org/mediawiki/2018/7/77/T--Imperial_College--PMSelectrochemistry.png)