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<title>Constraction</title> | <title>Constraction</title> | ||
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Experiments</a><div class="dropdown-menu"><a class="text-white dropdown-item display-4" href="BasicParts.html">Over View</a><a class="text-white dropdown-item display-4" href="Project-Constraction.html" aria-expanded="false">Constraction</a><a class="text-white dropdown-item display-4" href="Particle-Production.html" aria-expanded="false">Particle Production<br></a><a class="text-white dropdown-item display-4" href="Functional-Evaluation.html" aria-expanded="false">Fuctional Evaluation of Particle</a><a class="text-white dropdown-item display-4" href="InterLab.html" aria-expanded="false">InterLab</a></div> | Experiments</a><div class="dropdown-menu"><a class="text-white dropdown-item display-4" href="BasicParts.html">Over View</a><a class="text-white dropdown-item display-4" href="Project-Constraction.html" aria-expanded="false">Constraction</a><a class="text-white dropdown-item display-4" href="Particle-Production.html" aria-expanded="false">Particle Production<br></a><a class="text-white dropdown-item display-4" href="Functional-Evaluation.html" aria-expanded="false">Fuctional Evaluation of Particle</a><a class="text-white dropdown-item display-4" href="InterLab.html" aria-expanded="false">InterLab</a></div> | ||
</li><li class="nav-item dropdown"><a class="nav-link link text-white dropdown-toggle display-4" href="page6.html" data-toggle="dropdown-submenu" aria-expanded="false"><span class="mbri-rocket mbr-iconfont mbr-iconfont-btn"></span>About Us</a><div class="dropdown-menu"><a class="text-white dropdown-item display-4" href="team.html">Team<br></a><a class="text-white dropdown-item display-4" href="attribution.html" aria-expanded="false">attribution</a></div></li></ul> | </li><li class="nav-item dropdown"><a class="nav-link link text-white dropdown-toggle display-4" href="page6.html" data-toggle="dropdown-submenu" aria-expanded="false"><span class="mbri-rocket mbr-iconfont mbr-iconfont-btn"></span>About Us</a><div class="dropdown-menu"><a class="text-white dropdown-item display-4" href="team.html">Team<br></a><a class="text-white dropdown-item display-4" href="attribution.html" aria-expanded="false">attribution</a></div></li></ul> | ||
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<section class="engine"><a href="https://mobirise.info/t">amp templates</a></section><section class="mbr-section content5 cid-r5GT6OoMC7 mbr-parallax-background" id="content5-p"> | <section class="engine"><a href="https://mobirise.info/t">amp templates</a></section><section class="mbr-section content5 cid-r5GT6OoMC7 mbr-parallax-background" id="content5-p"> | ||
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<h2 class="align-center mbr-bold mbr-white pb-3 mbr-fonts-style display-1">Constraction</h2> | <h2 class="align-center mbr-bold mbr-white pb-3 mbr-fonts-style display-1">Constraction</h2> | ||
<h3 class="mbr-section-subtitle align-center mbr-light mbr-white pb-3 mbr-fonts-style display-5">For the pseudovirus production, we prepared structural gene, and non-structural gene with fluorescence protein gene.<div><br></div></h3> | <h3 class="mbr-section-subtitle align-center mbr-light mbr-white pb-3 mbr-fonts-style display-5">For the pseudovirus production, we prepared structural gene, and non-structural gene with fluorescence protein gene.<div><br></div></h3> | ||
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<a href="https://static.igem.org/mediawiki/2018/7/7c/T--Tokyo_Tech--const_parts.png"><img src="assets/images/jumbotron2.jpg" width="1400" alt="Mobirise" title=""></a> | <a href="https://static.igem.org/mediawiki/2018/7/7c/T--Tokyo_Tech--const_parts.png"><img src="assets/images/jumbotron2.jpg" width="1400" alt="Mobirise" title=""></a> | ||
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<h2 class="align-center pb-3 mbr-fonts-style display-2"><div><br></div><div>Results (Electrophoresis)</div><div><br></div></h2> | <h2 class="align-center pb-3 mbr-fonts-style display-2"><div><br></div><div>Results (Electrophoresis)</div><div><br></div></h2> | ||
<h3 class="mbr-section-subtitle align-center mbr-light mbr-fonts-style display-5">As shown in Figure, we successfully obtained each part, ranging from C and prM-E to FP-FMDV2a with non-structural region.<br><br><a href="http://parts.igem.org/File:TokyoTech_2018_EPresult.png">http://parts.igem.org/File:TokyoTech_2018_EPresult.png</a><div><br></div></h3> | <h3 class="mbr-section-subtitle align-center mbr-light mbr-fonts-style display-5">As shown in Figure, we successfully obtained each part, ranging from C and prM-E to FP-FMDV2a with non-structural region.<br><br><a href="http://parts.igem.org/File:TokyoTech_2018_EPresult.png">http://parts.igem.org/File:TokyoTech_2018_EPresult.png</a><div><br></div></h3> | ||
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Revision as of 05:57, 17 October 2018
<!DOCTYPE html>
Constraction
For the pseudovirus production, we prepared structural gene, and non-structural gene with fluorescence protein gene.
About structural gene, capsid (C), membrane (prM) and envelope (E) are necessary for the formation of viral structure. We prepared pCAG-C and pCAG-prM-E for Serotype I to IV. On the other hand, about non-structural gene with fluorescence protein gene, we prepared EGFP-FMDV2a, DsRed-Express-FMDV2a, ZsYellow-FMDV2a and AmCyan-FMDV2a, and inserted each of them to non-structural genes of dengue virus.
Since these plasmids are introduced into mammalian cells, the promoter and backbone should be optimized for eukaryotic cells. Our team uses pCAG vector which includes CAG promoter to induce high expression of interesting genes in mammalian cells, and pCMV for non-structural region.