Difference between revisions of "Team:William and Mary/Notebook"
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<li> Planned out several IFFLs to test. </li> | <li> Planned out several IFFLs to test. </li> | ||
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Revision as of 06:28, 17 October 2018
Notebook
Week 1
- Began cloning 3G parts based off the library sent from the Murray lab
- Researched literature on dual inducible promoters
- Sent out several cloned 3G parts for sequencing
- Planned out several IFFLs to test.
Week 1
- Continued cloning 3G parts onto our standard WM Pad backbone
- Redesigned primers for cloning 3G promoter parts, as previous ones were faulty
- Received results from sequencing, confirmed parts
- Beginning to assemble circuits using 3G assembly
- Making inducible reporter construct and an mf-Lon based IFFL
Week 3: 180611-180617
- Completed cloning the portion of our comprehensive 3G assembly part library needed for our circuits
- Adding remaining 3G parts from Murray’s lab to our library
- Working on designing and building a dual inducible promoter and low copy backbone for assembly
- Confirmed minipreps based on sequencing results
- Started plate reader for experiments on circuits with IPTG and ATC inducer
- Started using FACS machine for experiments