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<h1 class="text-center mg-lg tc-white ">

<span style="font-size:80%;">A CRISPR/Cas Base-Editing Toolkit </span>

From Genome to Transcriptome, From Detection to Modification.

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<span style="font-size:80%;">A Continuation and Beyond.</span>

<span style="font-size:80%;">CRISPR/Cas9 DNA Base Editor</span>

<div style="text-align: left;"><div style="line-height: 200%;">With the successful truncated dCas9-mediated transcriptional activation last year, we moved further this year into DNA base editing using CRISPR/Cas9 system. We also improved our truncated dCas9 further with novel strategies for higher binding efficiency.</div></div>

</p><a href="index.html" class="btn btn-wire btn-rd pull-left btn-lg" style="margin-top: 5%;">Project Improve</a>

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<span style="font-size:80%;">Human-Centred Research</span>

<div style="text-align: left;"><div style="line-height: 200%;">Our research involves not only the students and scientists, but also the Singapore public. While doing our project, we looked into the public opinions towards gene editing. Our human practice tells how&nbsp;our project evolved around the local community that we live in.&nbsp;</div></div>

</p><a href="index.html" class="btn btn-wire btn-rd" style="margin-top: 5%;">Integrated Human Practice</a>

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<span style="font-size:80%;">Adapt to Change.</span>

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<span style="font-size:80%;">CRISPR/Cas13b RNA Base Editor</span>

<div style="text-align: left;"><div style="line-height: 200%;">As we have learnt through our interviews with medical doctors and interaction with the public, it is evident that RNA editing is more promising and better accep-ted in our society. Hence, we reflected on our project and ventured into RNA editing.</div></div>

</p><a href="index.html" class="btn btn-wire btn-rd pull-left">Project REPAIR</a>

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<span style="font-size:80%;">Aim Even Higher.</span>

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<span style="font-size:80%;">Modification-Sensitive&nbsp;Nanopore Sequencing</span>

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<div style="text-align: left;"><div style="line-height: 200%;">To address the significant concern around the safety of RNA editing technology, we designed an unbiased, accurate RNA sequencing tool. Expanding the nanopore sequencing platform, we hope to enhance the saftey of RNA editing.</div></div>

</p><a href="index.html" class="btn btn-wire btn-rd pull-left" style="margin-top: 5%;">Project Nanopore</a>