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| | <div class="column full_size"> | | <div class="column full_size"> |
| | <h1>Basic Parts</h1> | | <h1>Basic Parts</h1> |
| − | <p>
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| − | A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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| − | </p>
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| − |
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| − | <p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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| − | </div>
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| − |
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| − | <partinfo>BBa_K2837000 short</partinfo>
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| − |
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| − | ===Name: ===
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| − | Phenylpropanoyltransferase (BAPT, TAX7)
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| − |
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| − | ===Reaction: ===
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| − | baccatin III + (3R)-3-amino-3-phenylpropanoyl-CoA → N-debenzoyl-(3'-RS)-2'-deoxytaxol + coenzyme A
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| − |
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| − | ===Km: ===
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| − | 5.6 uM for (3R)-3-amino-3-phenylpropanoyl-CoA;
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| − | 68 uM for baccatin III;
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| − | 2.4 uM for baccatin III and 4.9 uM beta-phenylalanoyl-CoA (Thornburg, 2015)
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| − |
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| − | ===Kcat: ===
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| − | 0.0583 ± 0.0010 s<sup>-1</sup> (Thornburg, 2015)
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| − |
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| − | ===Enzyme Description: ===
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| − | BAPT is the enzyme that adds the C13 Taxol side chain partially formed to the Taxol backbone. It makes use of <br>
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| − | CoA chemistry to add the side chain to the 13’ hydroxyl group of baccatin III.
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| − | <partinfo>BBa_K2837000 SequenceAndFeatures</partinfo>
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| − |
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| − | ===Design Notes===
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| − | Part digested from vector designed by Duke's 2016 team. Original vector constructed by Gibson Assembly of BAPT Gblock into pSMART-HC-Amp vector.
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| − |
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| − | ===Source===
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| − |
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| − | Origin from Pacific Yew Tree, Taxus brevifolia
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| − |
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| − | ===References===
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| − | Thornburg, C. (2015). Development of a four-step semi-biosynthesis of the anticancer drug paclitaxel and its analogues (Doctoral dissertation, Michigan State University).
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| | | | |
| | + | <object data=File:T--Duke--PSB1C3-BAPT_Plasmid_Map.gb width="100%" height="700"> </object> |
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| | <!-- | | <!-- |
