Difference between revisions of "Team:William and Mary/Demonstrate"

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<h3>★  ALERT! </h3>
 
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
 
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
 
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<h1 style="color: black;text-align:center;">Demonstrate</h1>
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<h1>Demonstrate</h1>
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Throughout our project, we have successfully introduced a new cloning method, developed and validated several protocols, and collected data that matches our mathematical models.  
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<h3>Gold Medal Criterion #4</h3>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve their gold medals!
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Please see the <a href="https://2018.igem.org/Judging/Medals">2018 Medals Page</a> for more information.
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3G Assembly has proven to be an accurate and efficient way to create circuits, not only in our lab, but in our <a href='https://2018.igem.org/Team:William_and_Mary/3G#COLLAB' style= 'color:green;'>collaborators’</a> as well. All twenty sequence confirmed circuits we are <a href='https://2018.igem.org/Team:William_and_Mary/Parts' style= 'color:green;'>submitting</a> to the registry were created using 3G assembly, demonstrating that it is a reliable method of DNA assembly.
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Along with our <a href='https://2018.igem.org/Team:William_and_Mary/3GProtocols' style= 'color:green;'>3G Protocol</a> and description of <a href='https://2018.igem.org/Team:William_and_Mary/3G_Mixed' style= 'color:green;'>Mixed 3G Assembly</a>, we created several other procedures to improve experiments in our lab. First, we developed a <a href='https://2018.igem.org/Team:William_and_Mary/Flow_Protocol' style= 'color:green;'>protocol</a> that allows for the freezing of cells before flow cytometry measurements are performed. This decreases time and labor costs and allows teams without flow cytometers to send their samples to other institutions for measurement. In addition, we designed and validated a <a href='https://2018.igem.org/Team:William_and_Mary/Results#INDUCERS' style= 'color:green;'>method</a> to dynamically remove inducer from media. Using this method, we biologically <a href='https://2018.igem.org/Team:William_and_Mary/Results#STAIRCASE' style= 'color:green;'>confirmed</a> our mathematical model predicting that an incoherent feedforward loop will produce a stepwise output from a pulsatile input of inducer.
 
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Latest revision as of 21:05, 17 October 2018

Demonstrate

Throughout our project, we have successfully introduced a new cloning method, developed and validated several protocols, and collected data that matches our mathematical models.
3G Assembly has proven to be an accurate and efficient way to create circuits, not only in our lab, but in our collaborators’ as well. All twenty sequence confirmed circuits we are submitting to the registry were created using 3G assembly, demonstrating that it is a reliable method of DNA assembly.
Along with our 3G Protocol and description of Mixed 3G Assembly, we created several other procedures to improve experiments in our lab. First, we developed a protocol that allows for the freezing of cells before flow cytometry measurements are performed. This decreases time and labor costs and allows teams without flow cytometers to send their samples to other institutions for measurement. In addition, we designed and validated a method to dynamically remove inducer from media. Using this method, we biologically confirmed our mathematical model predicting that an incoherent feedforward loop will produce a stepwise output from a pulsatile input of inducer.