Difference between revisions of "Team:Linkoping Sweden/Safety"

(Prototype team page)
 
 
(26 intermediate revisions by 4 users not shown)
Line 1: Line 1:
−
{{Linkoping_Sweden}}
+
{{Linkoping_Sweden/fixes}}
 +
{{Linkoping_Sweden/jsfixes}}
 +
{{Linkoping_Sweden/teststyle}}
 +
 
 
<html>
 
<html>
−
 
+
<head>
−
 
+
  <meta charset="utf-8">
−
<div class="column full_size">
+
  <meta name="viewport" content="width=device-width, initial-scale=1">
−
 
+
  <title>LiU iGEM</title>
−
<h1> Safety </h1>
+
</head>
−
<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
+
<body>
−
 
+
<header>
−
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
+
<a href="https://2018.igem.org/Team:Linkoping_Sweden">
−
 
+
    <img src="https://static.igem.org/mediawiki/2018/a/af/T--Linkoping_Sweden--helvitlogo.png" alt="iGEM-logo" class="logo" </a>  
−
</div>
+
<nav>
−
 
+
<ul class="liu-menu">
−
 
+
        <li><a href="/Team:Linkoping_Sweden">Home</a></li>
−
<div class="column two_thirds_size">
+
        <li class="dropdown">
−
<h3>Safe Project Design</h3>
+
            <a  href="/Team:Linkoping_Sweden/Human_Practices" class="dropbutton">Human Practices</a>
−
 
+
            <div class="dropdown-content">
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
              <a href="/Team:Linkoping_Sweden/Public_Engagement">Public Engagement</a>
−
 
+
            </div></li>
 +
        <li class="dropdown">
 +
            <a  href="/Team:Linkoping_Sweden/Collaborations" class="dropbutton">Collaborations</a></li>
 +
        <li class="dropdown">
 +
            <a  href="/Team:Linkoping_Sweden/Team" class="dropbutton">Team</a>
 +
            <div class="dropdown-content">
 +
            <a href="/Team:Linkoping_Sweden/Contact">Contact</a>
 +
            </div>
 +
            </li>
 +
        <li class="dropdown">
 +
            <a  href="/Team:Linkoping_Sweden/Project" class="dropbutton">Project</a>
 +
            <div class="dropdown-content">
 +
              <a href="/Team:Linkoping_Sweden/Model">Model</a>
 +
              <a href="/Team:Linkoping_Sweden/Notebook">Notebook</a>
 +
              <a href="/Team:Linkoping_Sweden/Design">Design</a>
 +
              <a href="/Team:Linkoping_Sweden/Experiments">Experiments</a>
 +
           
 +
              <a href="/Team:Linkoping_Sweden/Parts">Parts</a>
 +
              <a href="/Team:Linkoping_Sweden/Safety">Safety</a>
 +
              <a href="/Team:Linkoping_Sweden/Demonstrate">Demonstrate</a>
 +
              <a href="/Team:Linkoping_Sweden/Improve">Improve</a>
 +
            </div></li>
 +
        <li class="dropdown">
 +
            <a  href="/Team:Linkoping_Sweden/Attributions" class="dropbutton">Attributions</a>
 +
            <div class="dropdown-content">
 +
              <a href="/Team:Linkoping_Sweden/Sponsors">Sponsors</a>
 +
            </div></li>
 +
      </ul>
 +
</nav>
 +
</header>
 +
<section class="superproject">
 +
<h1>Safety</h1>
 +
</section>
 +
<section class="standard-text-section">
 +
<div class="standard-container">
 +
<h3>
 +
Bacterial Strains
 +
</h3>
 +
<h4>
 +
We chose to conduct our experiments on the bacteria Escherichia coli. The strains that were used were:
 +
</h4>
 
<ul>
 
<ul>
−
<li>Choosing a non-pathogenic chassis</li>
+
<li>BL21 DE3 Gold</li>
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
<li>XL1 Blue</li>
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
<li>NEB5 Alfa</li>
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
 
</ul>
 
</ul>
−
 
+
<h4>
 +
All of these strains are classified as Class 1 and therefore follows the iGEM regulations.
 +
</h4>
 +
<h3>
 +
Safe Lab Work
 +
</h3>
 +
<h4>
 +
When working in a lab, it is crucial to know the risks with the different experiments and bacteria. Therefore, it is important to have a daily routine that every team member follows. Since we have only been working with species from risk group 1, there have not been any major risks.<br>
 +
<br>
 +
<img class="responsive-img center-margin" src="https://static.igem.org/mediawiki/2018/d/d7/T--Linkoping_Sweden--safety1.jpg" alt="safety1" style="width:40%; margin-left: 10px; margin-bottom: 2px; margin-top:10px; border-radius: 8px; float:right"/>
 +
Our daily routine has involved always wearing lab coats and gloves, and if necessary, we have also used safety glasses. When working with DNA and toxic chemicals, we worked in a fume hood to make sure that no contamination would occur. <br><br>
 +
In the case of working with an open flame, no team member was to be left alone with the fire.<br><br>
 +
When staining the agarose gel we used Ethidium Bromide (EtBr), which is a dangerous cancerogenic chemical. To minimize the risk we kept the liquid in a container which had a permanent place in a fume hood. When in use we open the lid of the container in order to slowly place the agarose gel in the EtBr. Immediately afterwards we closed the lid and proceeded with staining our gel. When working with EtBr it is required to use gloves and after use, the gloves have to be thrown away in a special bin.<br><br>
 +
During the project, we have used liquid nitrogen to freeze and lyse our organisms. To prevent any accidents, two of our team members went through an education how to handle this liquid safely.
 +
<br><br>
 +
<h4>
 +
Every day after an experiment, we sterilised the lab benches and washed the equipment. Waste was thrown in a bin with special waste. No bacteria were ever thrown into the sink because of potential risk of spreading antibiotic resistance. If discarded, bacteria were thrown in a glass container where they remained until the glass container was full. At that point, the container was autoclaved in order to sterilise the bacteria-filled liquid.<br>
 +
<br>
 +
We have also used three different antibiotics: Ampicilin, Chlorampehicol and Kanamycin.
 +
Chloramphenicol and Ampicillin can both be autoclaved and then flushed down the drain. Since Kanamycin can tolerate heat/autoclaving and have unknown properties it must be submitted for combustion to make it harmless.
 +
</h4>
 +
<h3>
 +
Safe Shipment
 +
</h3>
 +
<h4>
 +
When sending our DN, for sequencing and for DNA Submission for the iGEM-registry we followed all protocols and did not encounter any safety concerns.
 +
</h4>
 
</div>
 
</div>
−
 
+
<div class="line-separator"></div>
−
<div class="column third_size">
+
</section>
−
<h3>Safe Lab Work</h3>
+
</body>
−
 
+
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
−
 
+
−
 
+
−
<h3>Safe Shipment</h3>
+
−
 
+
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
+
−
</div>
+
−
 
+
−
 
+
 
</html>
 
</html>

Latest revision as of 22:31, 17 October 2018

LiU iGEM

Safety

Bacterial Strains

We chose to conduct our experiments on the bacteria Escherichia coli. The strains that were used were:

  • BL21 DE3 Gold
  • XL1 Blue
  • NEB5 Alfa

All of these strains are classified as Class 1 and therefore follows the iGEM regulations.

Safe Lab Work

When working in a lab, it is crucial to know the risks with the different experiments and bacteria. Therefore, it is important to have a daily routine that every team member follows. Since we have only been working with species from risk group 1, there have not been any major risks.

safety1 Our daily routine has involved always wearing lab coats and gloves, and if necessary, we have also used safety glasses. When working with DNA and toxic chemicals, we worked in a fume hood to make sure that no contamination would occur.

In the case of working with an open flame, no team member was to be left alone with the fire.

When staining the agarose gel we used Ethidium Bromide (EtBr), which is a dangerous cancerogenic chemical. To minimize the risk we kept the liquid in a container which had a permanent place in a fume hood. When in use we open the lid of the container in order to slowly place the agarose gel in the EtBr. Immediately afterwards we closed the lid and proceeded with staining our gel. When working with EtBr it is required to use gloves and after use, the gloves have to be thrown away in a special bin.

During the project, we have used liquid nitrogen to freeze and lyse our organisms. To prevent any accidents, two of our team members went through an education how to handle this liquid safely.

Every day after an experiment, we sterilised the lab benches and washed the equipment. Waste was thrown in a bin with special waste. No bacteria were ever thrown into the sink because of potential risk of spreading antibiotic resistance. If discarded, bacteria were thrown in a glass container where they remained until the glass container was full. At that point, the container was autoclaved in order to sterilise the bacteria-filled liquid.

We have also used three different antibiotics: Ampicilin, Chlorampehicol and Kanamycin. Chloramphenicol and Ampicillin can both be autoclaved and then flushed down the drain. Since Kanamycin can tolerate heat/autoclaving and have unknown properties it must be submitted for combustion to make it harmless.

Safe Shipment

When sending our DN, for sequencing and for DNA Submission for the iGEM-registry we followed all protocols and did not encounter any safety concerns.