Difference between revisions of "Team:Toronto"

 
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{{Toronto}}
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{{Template:Toronto/CSS}}
 
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.grid-container {
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  display: grid;
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  grid-template-columns: 300px 300px 300px 300px;
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  grid-gap: 30px;
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  border-radius: 0px;
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  padding-bottom: 30px;
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  padding-top: 30px;
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  align-items: stretch;
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.grid-container > div {
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  background-color: rgba(15,65,92,1.0);
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  border: 3px solid white;
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border-radius: 0%;
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  text-align: center;
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  padding: 50px 20px;
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  font-size: 30px;
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  line-height: 30px;
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}
  
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}
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.timeline-wrapper {
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  background: linear-gradient(#000 10%, #283048 60%, #55679a 70%, rgba(15,65,92,1.0));
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}
  
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.center {
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    position: relative;
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}
  
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<div class="column full_size" >
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.centerPacker{
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<h1> Welcome to iGEM 2018! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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  top: 50%;
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<!--
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h1 {
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<img src="http://placehold.it/1080x320/c4baba/e4dede">
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  font-size: 40px;
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<img src="T--Toronto--logo.png" alt="tester_cloud">
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  line-height: 50px;
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-->
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  word-break: break-all;
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<video width="1080" height="320" src="https://i.imgur.com/3tFIv4d.mp4" alt="Testing_Gifs_And_MP4" autoPlay=True>
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}
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</style>
  
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</div>  
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<!--{{Toronto}}-->
  
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<!--PLACEHOLDER WEBPAGE (as the team decides what to do, this temporary webpage that likely does not yet fully abide all
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iGEM rules has been put in place-->
  
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<div class="column full_size" >
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<body>
  
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<h3>Before you start</h3>
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<div style="background-color: rgba(73,175,188,1.0);">
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<p> Please don't read the following pages:</p>
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<ul>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<div class="clear extra_space"></div>
 
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<div class="line_divider"></div>
 
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<div class="clear extra_space"></div>
 
  
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  <div style="color:white;text-align: center;padding-top:350px;padding-bottom:0px;background-image:url('https://static.igem.org/mediawiki/2018/2/23/T--Toronto--2018_Homepagebanner2.png'); background-color:rgba(255,255,255,1.0); background-repeat: no-repeat; background-size: cover;">
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    <div style="width:100%; margin:0 auto; background-color:rgba(0,0,0,0.7); padding-top: 30px; padding-bottom: 30px; color:white;text-align: center;">
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      <h1 style="background-color:rgba(0,0,0,0.0); color:white; font-family: 'Trebuchet MS', 'Lucida Grande', 'Lucida Sans Unicode', 'Lucida Sans', Tahoma, sans-serif; font-size:40px; line-height:40px;">
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      <img src="https://static.igem.org/mediawiki/2017/f/f1/T--Toronto--2017_igemTO2017-logo.svg" alt="Logo" style="width:600px;">
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      </br></br>Flotation, Separation, Bio-remediation</h1>
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    </div>
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  </div>
  
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  <!--
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  <div style="width:100%;color:white;text-align: center;padding-top:40px; padding-bottom:40px; background-color:rgba(0,0,0,1); background-image: linear-gradient(to bottom, black, black);">
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    <h1 style="background-color:rgba(0,0,0,0.0); color:white; font-family: "Trebuchet MS", "Lucida Grande", "Lucida Sans Unicode", "Lucida Sans", Tahoma, sans-serif;">Home of Toronto's 2018 Team</h1>
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  </div>
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  -->
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  <div style="background-color: rgba(0,0,0,0.0);">
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  <div class="center" style="height: 400px; width: 80%;">
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  <div class="centerPacker">
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  <div class="grid-container">
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    <div class="item3">
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      <h1 style="text-align: center;">
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        <a style="text-align: center; color: rgba(255, 255, 255, 1.0);" href="https://2018.igem.org/Team:Toronto/Project">
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            </br>
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            PROJECT 
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        </a>
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      </h1>
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    </div>
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  <div class="item1">
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<h1 style="text-align: center;">
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      <a style="text-align: center; color: rgba(255, 255, 255, 1.0);" href="https://2018.igem.org/Team:Toronto/PolicyAndPractices">
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    POLICY </br>
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          AND </br>
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          PRACTICES
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      </a>
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    </h1>
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</div>
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  <div class="item2">
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<h1 style="text-align: center;">
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      <a style="text-align: center; color: rgba(255, 255, 255, 1.0);" href="https://2018.igem.org/Team:Toronto/DryLab">
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          </br>
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          DRYLAB   
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      </a>
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    </h1>
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</div>
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<div class="item3">
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    <h1 style="text-align: center;">
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      <a style="text-align: center; color: rgba(255, 255, 255, 1.0);" href="https://2018.igem.org/Team:Toronto/WetLab">
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          </br>
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          WETLAB   
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      </a>
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    </h1>
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</div>
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  </div>
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  </div>
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  </div>
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  </div>
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<div style="background-color: rgba(15,65,92,1.0); background-image: linear-gradient(#1f263c, #000000);">
  
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<div class="column full_size" >
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<div class="center" style="height: 550px; width: 80%;">
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<h3> Styling your wiki </h3>
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<div class="centerPacker">
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<h1 style="color: rgba(255,255,255,1.0); font-size: 50px; line-height:50px;">ABOUT US</h1>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<p style="color: rgba(255,255,255,1.0); font-size: 20px; line-height:20px; text-align: center;">Our project focuses on demonstrating flotation of <i>Escherichia coli</i> using gas vesicle proteins (GvPs) as a novel cellular separation technique for bioremediation processes. Current techniques used in different industries such as mining and municipal wastewater treatment, are mechanical, harmful to the environment and expensive. We propose that our system will be a more cost-effective separation technique for various bioremediation processes.
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</br></br>
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</div>
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Previous iGEM teams have demonstrated gas vesicle production and flotation in mammalian and yeast cells using GvPs from various bacterial species. Shapiro <i>et al.</i>, (2018) engineered a GvP-producing plasmid using arg1 from <i>Aphanizomenon flos-aquae</i> and <i>Bacillus megaterium</i> to synthesize these echogenic structures and observed that high expression enabled <i>E. coli</i> to float.
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</br></br>
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Our goal is to replicate and improve their flotation results by modifying arg1 to achieve consistent flotation using a specific induction protocol. Upon sorption or uptake of pollutants or valuable materials, this technique could allow for simpler extraction of pollutant-harboring or heavy metal-bound bacteria.</p>
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<h3> Uploading pictures and files </h3>
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<a href="https://2018.igem.org/Special:Upload">
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UPLOAD FILES
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<h3> Wiki template information </h3>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h3> Editing your wiki </h3>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<div class="button_link">
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<a href="https://2018.igem.org/wiki/index.php?title=Team:Toronto&action=edit">
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EDIT PAGE
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<h3>Tips</h3>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
+
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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  <div style="width:100%;height:60px;background-color:rgba(0,0,0,1.0);color:white;text-align: center; float:left; padding-bottom:100px;">
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    <h1 style="background-color:rgba(0,0,0,0.0);color:black;font: bold 40px/80px Helvetica, Sans-Serif; ">footer 2018</h1>
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  </div>
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</body>
 
</html>
 
</html>

Latest revision as of 23:38, 17 October 2018

Logo

Flotation, Separation, Bio-remediation

ABOUT US

Our project focuses on demonstrating flotation of Escherichia coli using gas vesicle proteins (GvPs) as a novel cellular separation technique for bioremediation processes. Current techniques used in different industries such as mining and municipal wastewater treatment, are mechanical, harmful to the environment and expensive. We propose that our system will be a more cost-effective separation technique for various bioremediation processes.

Previous iGEM teams have demonstrated gas vesicle production and flotation in mammalian and yeast cells using GvPs from various bacterial species. Shapiro et al., (2018) engineered a GvP-producing plasmid using arg1 from Aphanizomenon flos-aquae and Bacillus megaterium to synthesize these echogenic structures and observed that high expression enabled E. coli to float.

Our goal is to replicate and improve their flotation results by modifying arg1 to achieve consistent flotation using a specific induction protocol. Upon sorption or uptake of pollutants or valuable materials, this technique could allow for simpler extraction of pollutant-harboring or heavy metal-bound bacteria.

footer 2018