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− | {{Toronto}} | + | {{Template:Toronto/CSS}} |
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<style> | <style> | ||
− | + | .grid-container { | |
− | + | display: grid; | |
− | + | grid-template-columns: 300px 300px 300px 300px; | |
+ | grid-gap: 30px; | ||
+ | background-color: rgba(0,0,0,0.0); | ||
+ | border-radius: 0px; | ||
+ | padding-bottom: 30px; | ||
+ | padding-top: 30px; | ||
+ | align-items: stretch; | ||
+ | } | ||
+ | .grid-container > div { | ||
+ | background-color: rgba(15,65,92,1.0); | ||
+ | border: 3px solid white; | ||
+ | border-radius: 0%; | ||
+ | text-align: center; | ||
+ | padding: 50px 20px; | ||
+ | font-size: 30px; | ||
+ | line-height: 30px; | ||
} | } | ||
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− | + | } | |
+ | .timeline-wrapper { | ||
+ | position: relative; | ||
+ | padding-bottom: 25em; | ||
+ | background: linear-gradient(#000 10%, #283048 60%, #55679a 70%, rgba(15,65,92,1.0)); | ||
+ | } | ||
− | + | .center { | |
− | + | position: relative; | |
− | + | } | |
− | + | .centerPacker{ | |
− | + | margin: 0; | |
− | + | position: absolute; | |
− | + | top: 50%; | |
− | + | left: 50%; | |
− | + | -ms-transform: translate(-50%, -50%); | |
− | + | transform: translate(-50%, -50%); | |
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+ | h1 { | ||
+ | font-size: 40px; | ||
+ | line-height: 50px; | ||
+ | word-break: break-all; | ||
+ | } | ||
+ | </style> | ||
+ | <!--{{Toronto}}--> | ||
+ | <!--PLACEHOLDER WEBPAGE (as the team decides what to do, this temporary webpage that likely does not yet fully abide all | ||
+ | iGEM rules has been put in place--> | ||
+ | <body> | ||
− | <div | + | <div style="background-color: rgba(73,175,188,1.0);"> |
− | + | ||
− | + | ||
− | <div | + | <div style="color:white;text-align: center;padding-top:350px;padding-bottom:0px;background-image:url('https://static.igem.org/mediawiki/2018/2/23/T--Toronto--2018_Homepagebanner2.png'); background-color:rgba(255,255,255,1.0); background-repeat: no-repeat; background-size: cover;"> |
− | + | <div style="width:100%; margin:0 auto; background-color:rgba(0,0,0,0.7); padding-top: 30px; padding-bottom: 30px; color:white;text-align: center;"> | |
− | + | <h1 style="background-color:rgba(0,0,0,0.0); color:white; font-family: 'Trebuchet MS', 'Lucida Grande', 'Lucida Sans Unicode', 'Lucida Sans', Tahoma, sans-serif; font-size:40px; line-height:40px;"> | |
− | + | <img src="https://static.igem.org/mediawiki/2017/f/f1/T--Toronto--2017_igemTO2017-logo.svg" alt="Logo" style="width:600px;"> | |
− | + | </br></br>Flotation, Separation, Bio-remediation</h1> | |
− | + | </div> | |
− | + | </div> | |
− | < | + | |
− | < | + | |
− | < | + | |
− | + | ||
− | </ | + | |
− | </div> | + | |
+ | <!-- | ||
+ | <div style="width:100%;color:white;text-align: center;padding-top:40px; padding-bottom:40px; background-color:rgba(0,0,0,1); background-image: linear-gradient(to bottom, black, black);"> | ||
+ | <h1 style="background-color:rgba(0,0,0,0.0); color:white; font-family: "Trebuchet MS", "Lucida Grande", "Lucida Sans Unicode", "Lucida Sans", Tahoma, sans-serif;">Home of Toronto's 2018 Team</h1> | ||
+ | </div> | ||
+ | --> | ||
+ | <div style="background-color: rgba(0,0,0,0.0);"> | ||
+ | <div class="center" style="height: 400px; width: 80%;"> | ||
+ | <div class="centerPacker"> | ||
+ | <div class="grid-container"> | ||
+ | <div class="item3"> | ||
+ | <h1 style="text-align: center;"> | ||
+ | <a style="text-align: center; color: rgba(255, 255, 255, 1.0);" href="https://2018.igem.org/Team:Toronto/Project"> | ||
+ | </br> | ||
+ | PROJECT | ||
+ | </a> | ||
+ | </h1> | ||
+ | </div> | ||
+ | <div class="item1"> | ||
+ | <h1 style="text-align: center;"> | ||
+ | <a style="text-align: center; color: rgba(255, 255, 255, 1.0);" href="https://2018.igem.org/Team:Toronto/PolicyAndPractices"> | ||
+ | POLICY </br> | ||
+ | AND </br> | ||
+ | PRACTICES | ||
+ | </a> | ||
+ | </h1> | ||
+ | </div> | ||
+ | <div class="item2"> | ||
+ | <h1 style="text-align: center;"> | ||
+ | <a style="text-align: center; color: rgba(255, 255, 255, 1.0);" href="https://2018.igem.org/Team:Toronto/DryLab"> | ||
+ | </br> | ||
+ | DRYLAB | ||
+ | </a> | ||
+ | </h1> | ||
+ | </div> | ||
+ | <div class="item3"> | ||
+ | <h1 style="text-align: center;"> | ||
+ | <a style="text-align: center; color: rgba(255, 255, 255, 1.0);" href="https://2018.igem.org/Team:Toronto/WetLab"> | ||
+ | </br> | ||
+ | WETLAB | ||
+ | </a> | ||
+ | </h1> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div style="background-color: rgba(15,65,92,1.0); background-image: linear-gradient(#1f263c, #000000);"> | ||
− | <div class=" | + | <div class="center" style="height: 550px; width: 80%;"> |
− | <div class=" | + | <div class="centerPacker"> |
− | < | + | <h1 style="color: rgba(255,255,255,1.0); font-size: 50px; line-height:50px;">ABOUT US</h1> |
− | + | <p style="color: rgba(255,255,255,1.0); font-size: 20px; line-height:20px; text-align: center;">Our project focuses on demonstrating flotation of <i>Escherichia coli</i> using gas vesicle proteins (GvPs) as a novel cellular separation technique for bioremediation processes. Current techniques used in different industries such as mining and municipal wastewater treatment, are mechanical, harmful to the environment and expensive. We propose that our system will be a more cost-effective separation technique for various bioremediation processes. | |
− | + | </br></br> | |
− | + | Previous iGEM teams have demonstrated gas vesicle production and flotation in mammalian and yeast cells using GvPs from various bacterial species. Shapiro <i>et al.</i>, (2018) engineered a GvP-producing plasmid using arg1 from <i>Aphanizomenon flos-aquae</i> and <i>Bacillus megaterium</i> to synthesize these echogenic structures and observed that high expression enabled <i>E. coli</i> to float. | |
− | < | + | </br></br> |
− | + | Our goal is to replicate and improve their flotation results by modifying arg1 to achieve consistent flotation using a specific induction protocol. Upon sorption or uptake of pollutants or valuable materials, this technique could allow for simpler extraction of pollutant-harboring or heavy metal-bound bacteria.</p> | |
− | < | + | </div> |
− | < | + | </div> |
− | + | </div> | |
− | </ | + | |
− | </div> | + | |
− | </div> | + | |
+ | <div style="width:100%;height:60px;background-color:rgba(0,0,0,1.0);color:white;text-align: center; float:left; padding-bottom:100px;"> | ||
+ | <h1 style="background-color:rgba(0,0,0,0.0);color:black;font: bold 40px/80px Helvetica, Sans-Serif; ">footer 2018</h1> | ||
+ | </div> | ||
</body> | </body> | ||
</html> | </html> |
Latest revision as of 23:38, 17 October 2018
Flotation, Separation, Bio-remediation
ABOUT US
Our project focuses on demonstrating flotation of Escherichia coli using gas vesicle proteins (GvPs) as a novel cellular separation technique for bioremediation processes. Current techniques used in different industries such as mining and municipal wastewater treatment, are mechanical, harmful to the environment and expensive. We propose that our system will be a more cost-effective separation technique for various bioremediation processes. Previous iGEM teams have demonstrated gas vesicle production and flotation in mammalian and yeast cells using GvPs from various bacterial species. Shapiro et al., (2018) engineered a GvP-producing plasmid using arg1 from Aphanizomenon flos-aquae and Bacillus megaterium to synthesize these echogenic structures and observed that high expression enabled E. coli to float. Our goal is to replicate and improve their flotation results by modifying arg1 to achieve consistent flotation using a specific induction protocol. Upon sorption or uptake of pollutants or valuable materials, this technique could allow for simpler extraction of pollutant-harboring or heavy metal-bound bacteria.