Dry lab

• Developed an electrochemical model which simulates oxidised or reduced species being formed at and diffusing away from an electrode surface
• Developed a biological model which simulates the genetic response to the concentration of oxidised and reduced species on a single cell level
• Developed an integrated model and fitted it to experimental data to estimate absolute parameters of the electrogenetic system
• Designed and constructed an affordable electrode array to facilitate programmable spatial using the electrogenetic device.
• Developed a user-friendly phone application for remote control of patterning using the electrode array.

Wet lab

• Generated the first aerobic electrogenetic device in E. coli
• Optimised chemical conditions of the system to maximise the biological response of the electrogenetic device without significantly impacting cell viability.
• Proved this electrogenetic can provide fine spatial control of gene expression using a cheap electrode set-up.
• Created a library of electrogenetic parts for use in future electrogenetic project.
• Assembled the electrogenetic library into X different constructs using the next-generation BASIC assembly method, which were then characterised.
• Proved applications of electrogenetic devices in biocontainment and biomanifacturing.
• Found evidence of a new phenazine molecule as an inducer, which was suggested to exist in prior literature.

Human practices and outreach

• Proved molecules in the system could be replaced with safer alternatives following experts’ concerns of the toxicity of the system.
• Designed a Communinication Strategy Guide (CSG) to direct communication of our project with stakeholders
• Desined an interactive app (LTAT) to foster communication of problems within a team

Collaborations

• Collaborated with X different teams from X different countries.
• Proved the part library can be used for alternative devices to electrogenetic systems with Oxford University.
• Ensured comparability of the part library with multiple assembly methods with Oxford University