We successfully created an IFFL that utilizes the mf-Lon protease system, an extremely powerful and tunable system, since the degradation rate constant can be tuned by using different strength pdts. We tested the system with short temporal input in the small molecule induced IFFL. We were able to show that IFFLs do indeed function as temporal distinguishers.

we developed system of Ordinary Differential Equations (ODE) and assessed IFFL's decoding ability under noise. Results support IFFL's ability to decode time-domain information much more accurately than its counterpart as well as having superior fidelity to noise response.

See Human Practices Overview.