Difference between revisions of "Team:NUDT CHINA/Notebook"

 
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   <p style="font-size:36px;margin: 0 0%;padding: 5% 0 0 10%;color: white;">Designed Protein Degradation Method Based on</p>
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<p style="font-size:36px;margin: 0 0%;padding: 0 0 0 10%;color: white;">Trim21 And Nanobody&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;--&nbsp;Notebook</p>
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<div class="col-md-12 column" alt="cover_photo" style="width:100%;height:250px;background-size:cover;background-position:0px -350px; background-image:url(https://static.igem.org/mediawiki/2016/thumb/3/3f/NUDT_CHINA2016_FILES-img-banner-3.0.png/800px-NUDT_CHINA2016_FILES-img-banner-3.0.png);background-repeat:no-repeat;">
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   <p style="font-size:36px;margin: 0 0%;padding: 8% 0 0 10%;color: white;">Development of A Novel</p>
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<p style="font-size:36px;margin: 0 0%;padding: 0 0 0 10%;color: white;">Blood-MicroRNA Handy Detection System with CRISPR</p>
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</div>
 
</div>
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                           <div class="col-md-push-1 col-md-10">
 
                           <div class="col-md-push-1 col-md-10">
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                                       <h1 style="font-size: 40px;font-weight: 900"></h1>
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                                       <h1 style="font-size: 40px;font-weight: 1200"></h1>
 
                                       <p alt="content_add" style="font-size: 20px;">
 
                                       <p alt="content_add" style="font-size: 20px;">
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Chuanyang Liu, Changsong Gao, Chushu Zhu and Qijie Xu completed most of the experiments.
+
Jiaxin Ma ,Tianyi Zhang,Chenyu Lu and Chenxing Sun completed most of the experiments.
 
</p>
 
</p>
 
 
 
</div>
 
</div>
  
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<table class="table table-striped">
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<div class="col-md-push-1 col-md-10">
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  <caption>Fig.1 OD600 reference point</caption>
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−
 
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<center>
 
<center>
 
<table class="table table-striped">
 
<table class="table table-striped">
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  <caption>scx</caption>
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  <caption style="font-size: 25px">Jiaxin Ma</caption>
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
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<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
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<p><strong>6.15-7.4</strong></p>
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<p><strong>6.15-7.3</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Finish the culture of A549 cell and optimization of culture condition&nbsp;with Chuanyang Liu</p>
+
<p>Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Tianyi Zhang.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
−
<p><strong>7.5-</strong><strong>8.13</strong></p>
+
<p><strong>7.4-8.3</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Work on Western Blot assay for target gene expression&nbsp;with Chuanyang Liu</p>
+
<p>Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Tianyi Zhang.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
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<p><strong>8.</strong><strong>14-8.26</strong></p>
+
<p><strong>8.4-8.15</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Do Western Blot assay EMT markers&nbsp;with Chuanyang Liu</p>
+
<p>Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Tianyi Zhang.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
−
<p><strong>8.27-9.22</strong></p>
+
<p><strong>8.16-8.28</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Finish the Cell proliferation and migration assay of miR-214 Locker group&nbsp;with Chuanyang Liu</p>
+
<p>Work on repeated validation of GFP interfering efficiency with Tianyi Zhang.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
−
<p><strong>9.22-10.14</strong></p>
+
<p><strong>8.29-9.22</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Finish the Cell proliferation and migration assay of miR-654 Locker group&nbsp;with Chuanyang Liu</p>
+
<p>Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Tianyi Zhang.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="153">
 +
<p><strong>9.22-10.10</strong></p>
 +
</td>
 +
<td width="538">
 +
<p>Work on the proliferation and migration assay of cells with ErbB3 interfered with Tianyi Zhang.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
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</table>
 
</table>
 
</center>   
 
</center>   
−
 
+
</div>
−
<table class="table table-striped">
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<div class="col-md-push-1 col-md-10">
−
  <caption>Fig.1 OD600 reference point</caption>
+
−
 
+
 
<center>
 
<center>
−
<table>
+
<table class="table table-striped">
 +
  <caption style="font-size: 25px">Tianyi Zhang</caption>
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
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<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
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<p><strong>6.15-7.4</strong></p>
+
<p><strong>6.15-7.3</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Finish the Phosphorylation and annealing of the oligos&nbsp;with Qijie Xu</p>
+
<p>Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Jiaxin Ma. </p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
−
<p><strong>7.5-</strong><strong>8.13</strong></p>
+
<p><strong>7.4-8.3</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Work on miRNA locker assembly with Qijie Xu</p>
+
<p>Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Jiaxin Ma.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
−
<p><strong>8.</strong><strong>14-8.26</strong></p>
+
<p><strong>8.4-8.15</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Finish the Asymmetric PCR to generate ssDNAs from dsDNAs&nbsp;with Qijie Xu</p>
+
<p>Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Jiaxin Ma.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
−
<p><strong>8.27-9.22</strong></p>
+
<p><strong>8.16-8.28</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Construct parts&nbsp;with Qijie Xu</p>
+
<p>Work on repeated validation of GFP interfering efficiency with Jiaxin Ma.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
−
<p><strong>9.22-10.14</strong></p>
+
<p><strong>8.29-9.22</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Work on the experimental validation of parts&nbsp;with Qijie Xu</p>
+
<p>Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Jiaxin Ma.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="153">
 +
<p><strong>9.22-10.10</strong></p>
 +
</td>
 +
<td width="538">
 +
<p>Work on the proliferation and migration assay of cells with ErbB3 interfered with Jiaxin Ma.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
 
</table>
 
</table>
−
</center>
+
</center>
−
 
+
</div>
−
<table class="table table-striped">
+
<div class="col-md-push-1 col-md-10">
−
  <caption>Fig.1 OD600 reference point</caption>
+
<center>
 +
<table class="table table-striped">
 +
  <caption style="font-size: 25px">Chenyu Lu</caption>
 +
<tbody>
 +
<tr>
 +
<td width="153">
 +
<p><strong>D</strong><strong>ata</strong></p>
 +
</td>
 +
<td width="538">
 +
<p><strong>Project</strong></p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="153">
 +
<p><strong>6.15-7.3</strong></p>
 +
</td>
 +
<td width="538">
 +
<p>Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenxing Sun.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="153">
 +
<p><strong>7.4-8.3</strong></p>
 +
</td>
 +
<td width="538">
 +
<p>Hela Cell culture and preparatory works on fluorescence detection with Chenxing Sun. </p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="153">
 +
<p><strong>8.4-8.28</strong></p>
 +
</td>
 +
<td width="538">
 +
<p>Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenxing Sun.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="153">
 +
<p><strong>8.29-9.22</strong></p>
 +
</td>
 +
<td width="538">
 +
<p>Construct Parts with Chenxing Sun.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td width="153">
 +
<p><strong>9.22-10.10</strong></p>
 +
</td>
 +
<td width="538">
 +
<p>Work on the experimental validation of parts with Chenxing Sun.</p>
 +
</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
</center> 
 +
</div>
  
 +
<div class="col-md-push-1 col-md-10">
 
<center>
 
<center>
−
<table>
+
<table class="table table-striped">
 +
  <caption style="font-size: 25px">Chenxing Sun</caption>
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
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<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
−
<p><strong>6.15-7.4</strong></p>
+
<p><strong>6.15-7.3</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Finish the Phosphorylation and annealing of the oligos&nbsp;with Chushu Zhu</p>
+
<p>Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenyu Lu . </p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
−
<p><strong>7.5-</strong><strong>8.13</strong></p>
+
<p><strong>7.4-8.3</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Work on miRNA locker assembly with Chushu Zhu</p>
+
<p>Hela Cell culture and preparatory works on fluorescence detection with Chenyu Lu . </p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
−
<p><strong>8.</strong><strong>14-8.26</strong></p>
+
<p><strong>8.4-8.28</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Finish the Asymmetric PCR to generate ssDNAs from dsDNAs&nbsp;with Chushu Zhu</p>
+
<p>Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenyu Lu .</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
−
<p><strong>8.27-9.22</strong></p>
+
<p><strong>8.29-9.22</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Construct parts&nbsp;with Chushu Zhu</p>
+
<p>Construct Parts with Chenyu Lu .</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td width="153">
 
<td width="153">
−
<p><strong>9.22-10.14</strong></p>
+
<p><strong>9.22-10.10</strong></p>
 
</td>
 
</td>
 
<td width="538">
 
<td width="538">
−
<p>Work on the experimental validation of parts&nbsp;with Chushu Zhu</p>
+
<p>Work on the experimental validation of parts with Chenyu Lu .</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
−
</table></center>
+
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</center>
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{{NUDT_CHINA/footer}}
 
{{NUDT_CHINA/footer}}

Latest revision as of 01:55, 18 October 2018

Designed Protein Degradation Method Based on

Trim21 And Nanobody              -- Notebook

Jiaxin Ma ,Tianyi Zhang,Chenyu Lu and Chenxing Sun completed most of the experiments.

Jiaxin Ma

Data

Project

6.15-7.3

Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Tianyi Zhang.

7.4-8.3

Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Tianyi Zhang.

8.4-8.15

Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Tianyi Zhang.

8.16-8.28

Work on repeated validation of GFP interfering efficiency with Tianyi Zhang.

8.29-9.22

Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Tianyi Zhang.

9.22-10.10

Work on the proliferation and migration assay of cells with ErbB3 interfered with Tianyi Zhang.
Tianyi Zhang

Data

Project

6.15-7.3

Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Jiaxin Ma.

7.4-8.3

Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Jiaxin Ma.

8.4-8.15

Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Jiaxin Ma.

8.16-8.28

Work on repeated validation of GFP interfering efficiency with Jiaxin Ma.

8.29-9.22

Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Jiaxin Ma.

9.22-10.10

Work on the proliferation and migration assay of cells with ErbB3 interfered with Jiaxin Ma.
Chenyu Lu

Data

Project

6.15-7.3

Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenxing Sun.

7.4-8.3

Hela Cell culture and preparatory works on fluorescence detection with Chenxing Sun.

8.4-8.28

Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenxing Sun.

8.29-9.22

Construct Parts with Chenxing Sun.

9.22-10.10

Work on the experimental validation of parts with Chenxing Sun.
Chenxing Sun

Data

Project

6.15-7.3

Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenyu Lu .

7.4-8.3

Hela Cell culture and preparatory works on fluorescence detection with Chenyu Lu .

8.4-8.28

Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenyu Lu .

8.29-9.22

Construct Parts with Chenyu Lu .

9.22-10.10

Work on the experimental validation of parts with Chenyu Lu .