Difference between revisions of "Team:NUDT CHINA/Notebook"

 
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   <p style="font-size:36px;margin: 0 0%;padding: 5% 0 0 10%;color: white;">Designed Protein Degradation Method Based on</p>
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<p style="font-size:36px;margin: 0 0%;padding: 0 0 0 10%;color: white;">Trim21 And Nanobody&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;--&nbsp;Notebook</p>
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   <p style="font-size:36px;margin: 0 0%;padding: 8% 0 0 10%;color: white;">Designed Protein Degradation Method Based on</p>
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<p style="font-size:36px;margin: 0 0%;padding: 0 0 0 10%;color: white;">Trim21 And Nanobody</p>
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                                       <p alt="content_add" style="font-size: 20px;">
Chuanyang Liu, Changsong Gao, Chushu Zhu and Qijie Xu completed most of the experiments.
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Jiaxin Ma ,Tianyi Zhang,Chenyu Lu and Chenxing Sun completed most of the experiments.
 
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Latest revision as of 01:55, 18 October 2018

Designed Protein Degradation Method Based on

Trim21 And Nanobody              -- Notebook

Jiaxin Ma ,Tianyi Zhang,Chenyu Lu and Chenxing Sun completed most of the experiments.

Jiaxin Ma

Data

Project

6.15-7.3

Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Tianyi Zhang.

7.4-8.3

Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Tianyi Zhang.

8.4-8.15

Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Tianyi Zhang.

8.16-8.28

Work on repeated validation of GFP interfering efficiency with Tianyi Zhang.

8.29-9.22

Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Tianyi Zhang.

9.22-10.10

Work on the proliferation and migration assay of cells with ErbB3 interfered with Tianyi Zhang.

Tianyi Zhang

Data

Project

6.15-7.3

Construct plasmid expressing GFPnano-IgG1Fc and Trim21 with Jiaxin Ma.

7.4-8.3

Work on the optimization of transfection and fluorescence-detecting conditions in Hela and A549 cells with Jiaxin Ma.

8.4-8.15

Work on Western Blot assay for target gene (GFP) expression and fluorescence detection with Jiaxin Ma.

8.16-8.28

Work on repeated validation of GFP interfering efficiency with Jiaxin Ma.

8.29-9.22

Work on transfection optimization, EMT-related Markers detection of ErbB3-interfered cells with Jiaxin Ma.

9.22-10.10

Work on the proliferation and migration assay of cells with ErbB3 interfered with Jiaxin Ma.

Chenyu Lu

Data

Project

6.15-7.3

Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenxing Sun.

7.4-8.3

Hela Cell culture and preparatory works on fluorescence detection with Chenxing Sun.

8.4-8.28

Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenxing Sun.

8.29-9.22

Construct Parts with Chenxing Sun.

9.22-10.10

Work on the experimental validation of parts with Chenxing Sun.

Chenxing Sun

Data

Project

6.15-7.3

Validation of plasmid expression GFP-nano-IgGFc and Trim21, culture of Hela cells with Chenyu Lu .

7.4-8.3

Hela Cell culture and preparatory works on fluorescence detection with Chenyu Lu .

8.4-8.28

Construct plasmid expression ErbB3-IgG1Fc and trim21, and construct Parts with Chenyu Lu .

8.29-9.22

Construct Parts with Chenyu Lu .

9.22-10.10

Work on the experimental validation of parts with Chenyu Lu .