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<div class="col-md-12 column" alt="cover_photo" style="width:100%;height:250px;background-size:contain;0px -100px; background-image:url(https://static.igem.org/mediawiki/2018/c/c6/T--NUDT_CHINA--banner_model.jpg);background-repeat:no-repeat;">
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  <p style="font-size:36px;margin: 0 0%;padding: 5% 0 0 10%;color: white;">Designed Protein Degradation Method Based on</p>
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<p style="font-size:36px;margin: 0 0%;padding: 0 0 0 10%;color: white;">Trim21 And Nanobody&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;--&nbsp;Model</p>
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<a href="#model1"><img class="model1" src="https://static.igem.org/mediawiki/2016/c/c1/T--Manchester--modelling1_pic.png" ></img></a>
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<a href="#model2"><img class="model1" src="https://static.igem.org/mediawiki/2016/a/a3/T--Manchester--modelling2_pic.png" alt="What is esemble modelling?"></img></a>
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                                <div class="col-md-push-1 col-md-10">
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      <h1 style="font-size: 40px;font-weight: 900">Our Work</h1></br>
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<p alt="content_add" style="font-size: 18px;"> Our innovative project present a target protein disruption method in order to degrade endogenous target proteins acutely in mammalian cells. </br></br>
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To explain and predict the possibilities within the wetlab limits,we built ODE models and applied our measured data to parameters simulation method based on Bayesian parameter estimation to obtain parameters which fits our reality better. </br></br>
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We use this model to obtain the relation between the expression time of plasmid and protein degradation rate,making it accessible to future teams who want to incorporate our minds into their designs.</br></br>
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Through the modeling ,we understand the reaction mechanism more specifically and obtain how different factors influence the degradation process .This model also offers guidelines on choosing detection point and future clinical application.</br></br> </p>
  
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<a href="https://2018.igem.org/Team:NUDT_CHINA/Model/Basic Design"> <img alt="300x200" src="https://static.igem.org/mediawiki/2018/d/d0/T--NUDT_CHINA--our_work_da2.jpg" /> </a>
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<p alt="photo_detail" style="text-align: center;">Basic Work</p>
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<div class="col-md-6">
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<!-- <p class="text-center text-info">this is the sign of igem</p> -->
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<p alt="photo_detail" style="text-align: center;"><em>GFP</em></p>
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<div class="col-md-6">
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<!-- <p class="text-center text-info">this is the sign of igem</p> -->
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<p alt="photo_detail" style="text-align: center;"><em>ErbB3</em></p>
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                                        <div class="col-md-push-1 col-md-4 thumbnail">
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<a href="https://2018.igem.org/Team:NUDT_CHINA/Model/Deduction"> <img alt="300x200" src="https://static.igem.org/mediawiki/2018/2/2a/T--NUDT_CHINA--our_work_you2.png"> </a>
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                                        <div class="col-md-push-3 col-md-4 thumbnail">
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<a href="https://2018.igem.org/Team:NUDT_CHINA/Model/Deduction"> <img alt="300x200" src="https://static.igem.org/mediawiki/2018/d/da/T--NUDT_CHINA--our_work_you6.png"> </a>
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<a href="#model3"><img class="model1" src="https://static.igem.org/mediawiki/2016/4/46/T--Manchester--modelling3_pic.png" alt="What does our modelling achieve?"></img></a>
 
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<h1> Modeling</h1>
 
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<p>Mathematical models and computer simulations provide a great way to describe the function and operation of BioBrick Parts and Devices. Synthetic Biology is an engineering discipline, and part of engineering is simulation and modeling to determine the behavior of your design before you build it. Designing and simulating can be iterated many times in a computer before moving to the lab. This award is for teams who build a model of their system and use it to inform system design or simulate expected behavior in conjunction with experiments in the wetlab.</p>
 
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<h3> Gold Medal Criterion #3</h3>
 
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Convince the judges that your project's design and/or implementation is based on insight you have gained from modeling. This could be either a new model you develop or the implementation of a model from a previous team. You must thoroughly document your model's contribution to your project on your team's wiki, including assumptions, relevant data, model results, and a clear explanation of your model that anyone can understand.
 
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<br><br>
 
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The model should impact your project design in a meaningful way. Modeling may include, but is not limited to, deterministic, exploratory, molecular dynamic, and stochastic models. Teams may also explore the physical modeling of a single component within a system or utilize mathematical modeling for predicting function of a more complex device.
 
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Please see the <a href="https://2018.igem.org/Judging/Medals"> 2018
 
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Medals Page</a> for more information.
 
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<h3>Best Model Special Prize</h3>
 
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<p>
 
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To compete for the <a href="https://2018.igem.org/Judging/Awards">Best Model prize</a>, please describe your work on this page  and also fill out the description on the <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a>. Please note you can compete for both the gold medal criterion #3 and the best model prize with this page.
 
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<br><br>
 
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You must also delete the message box on the top of this page to be eligible for the Best Model Prize.
 
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<h3> Inspiration </h3>
 
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<p>
 
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Here are a few examples from previous teams:
 
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</p>
 
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<ul>
 
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<li><a href="https://2016.igem.org/Team:Manchester/Model">2016 Manchester</a></li>
 
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<li><a href="https://2016.igem.org/Team:TU_Delft/Model">2016 TU Delft</li>
 
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<li><a href="https://2014.igem.org/Team:ETH_Zurich/modeling/overview">2014 ETH Zurich</a></li>
 
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<li><a href="https://2014.igem.org/Team:Waterloo/Math_Book">2014 Waterloo</a></li>
 
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Latest revision as of 02:00, 18 October 2018

Designed Protein Degradation Method Based on

Trim21 And Nanobody              -- Model

Our Work


Our innovative project present a target protein disruption method in order to degrade endogenous target proteins acutely in mammalian cells.

To explain and predict the possibilities within the wetlab limits,we built ODE models and applied our measured data to parameters simulation method based on Bayesian parameter estimation to obtain parameters which fits our reality better.

We use this model to obtain the relation between the expression time of plasmid and protein degradation rate,making it accessible to future teams who want to incorporate our minds into their designs.

Through the modeling ,we understand the reaction mechanism more specifically and obtain how different factors influence the degradation process .This model also offers guidelines on choosing detection point and future clinical application.

300x200

Basic Work

GFP

ErbB3

300x200
300x200