Difference between revisions of "Team:NUDT CHINA/Model"

 
(10 intermediate revisions by 2 users not shown)
Line 110: Line 110:
 
</style>
 
</style>
 
<div class="col-md-12 column" alt="cover_photo" style="width:100%;height:250px;background-size:contain;0px -100px; background-image:url(https://static.igem.org/mediawiki/2018/c/c6/T--NUDT_CHINA--banner_model.jpg);background-repeat:no-repeat;">
 
<div class="col-md-12 column" alt="cover_photo" style="width:100%;height:250px;background-size:contain;0px -100px; background-image:url(https://static.igem.org/mediawiki/2018/c/c6/T--NUDT_CHINA--banner_model.jpg);background-repeat:no-repeat;">
−
   <p style="font-size:36px;margin: 0 0%;padding: 5% 0 0 10%;color: white;">Development of A Novel</p>
+
   <p style="font-size:36px;margin: 0 0%;padding: 5% 0 0 10%;color: white;">Designed Protein Degradation Method Based on</p>
−
<p style="font-size:36px;margin: 0 0%;padding: 0 0 0 10%;color: white;">Blood-MicroRNA Handy Detection System with CRISPR</p></div>
+
<p style="font-size:36px;margin: 0 0%;padding: 0 0 0 10%;color: white;">Trim21 And Nanobody&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;--&nbsp;Model</p>
 +
</div>
  
 
<div class="container">
 
<div class="container">
Line 118: Line 119:
 
<div class="row" style="margin-top: 25px">
 
<div class="row" style="margin-top: 25px">
 
                                 <div class="col-md-push-1 col-md-10">
 
                                 <div class="col-md-push-1 col-md-10">
−
      <h1 style="font-size: 40px;font-weight: 900">Our Work</h1>
+
      <h1 style="font-size: 40px;font-weight: 900">Our Work</h1></br>
−
<p alt="content_add" style="font-size: 18px;"> Our innovative project present a target protein disruption method in order to degrade endogenous target proteins acutely in mammalian cells.To explain and predict the possibilities within the wetlab limits，we built ODE models and applied our measured data to parameters simulation method based on Bayesian parameter estimation to obtain parameters which fits our reality better. We use this model to obtain the relation between the expression time of plasmid and protein degradation rate,making it accessible to future teams who want to incorporate our minds into their designs.Through the modeling ,we understand the reaction mechanism more specifically and obtain how different factors influence the degradation process .This model also offers guidelines on choosing detection point and future clinical application. </p>
+
<p alt="content_add" style="font-size: 18px;"> Our innovative project present a target protein disruption method in order to degrade endogenous target proteins acutely in mammalian cells. </br></br>
 +
To explain and predict the possibilities within the wetlab limits,we built ODE models and applied our measured data to parameters simulation method based on Bayesian parameter estimation to obtain parameters which fits our reality better. </br></br>
 +
We use this model to obtain the relation between the expression time of plasmid and protein degradation rate,making it accessible to future teams who want to incorporate our minds into their designs.</br></br>
 +
Through the modeling ,we understand the reaction mechanism more specifically and obtain how different factors influence the degradation process .This model also offers guidelines on choosing detection point and future clinical application.</br></br> </p>
  
 
</div>
 
</div>
 
<div class="col-md-12 column" alt = "main_content">
 
<div class="col-md-12 column" alt = "main_content">
 
<div class="col-md-8 col-md-push-2 thumbnail" alt="content_pictute">
 
<div class="col-md-8 col-md-push-2 thumbnail" alt="content_pictute">
−
<a href="https://2018.igem.org/Team:NUDT_CHINA/Model/Overview#model1"> <img alt="300x200" src="https://static.igem.org/mediawiki/2018/9/9b/2018_igem_menu_logo.svg" /> </a>  
+
<a href="https://2018.igem.org/Team:NUDT_CHINA/Model/Basic Design"> <img alt="300x200" src="https://static.igem.org/mediawiki/2018/d/d0/T--NUDT_CHINA--our_work_da2.jpg" /> </a>  
−
<p alt="photo_detail" style="text-align: center;">This is the model1!</p>
+
<p alt="photo_detail" style="text-align: center;">Basic Work</p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 131: Line 135:
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<!-- <p class="text-center text-info">this is the sign of igem</p> -->
 
<!-- <p class="text-center text-info">this is the sign of igem</p> -->
−
<p alt="photo_detail" style="text-align: center;"><em>model2</em></p>
+
<p alt="photo_detail" style="text-align: center;"><em>GFP</em></p>
 
</div>
 
</div>
 
<div class="col-md-6">
 
<div class="col-md-6">
 
<!-- <p class="text-center text-info">this is the sign of igem</p> -->
 
<!-- <p class="text-center text-info">this is the sign of igem</p> -->
−
<p alt="photo_detail" style="text-align: center;"><em>model3</em></p>
+
<p alt="photo_detail" style="text-align: center;"><em>ErbB3</em></p>
 
</div>
 
</div>
 
                                         <div class="col-md-push-1 col-md-4 thumbnail">
 
                                         <div class="col-md-push-1 col-md-4 thumbnail">
−
<a href="https://2018.igem.org/Team:NUDT_CHINA/Model/Overview#model2"> <img alt="300x200" src="https://static.igem.org/mediawiki/2018/9/9b/2018_igem_menu_logo.svg"> </a>
+
<a href="https://2018.igem.org/Team:NUDT_CHINA/Model/Deduction"> <img alt="300x200" src="https://static.igem.org/mediawiki/2018/2/2a/T--NUDT_CHINA--our_work_you2.png"> </a>
 
</div>
 
</div>
 
                                         <div class="col-md-push-3 col-md-4 thumbnail">
 
                                         <div class="col-md-push-3 col-md-4 thumbnail">
−
<a href="https://2018.igem.org/Team:NUDT_CHINA/Model/Overview#model3"> <img alt="300x200" src="https://static.igem.org/mediawiki/2018/9/9b/2018_igem_menu_logo.svg"> </a>
+
<a href="https://2018.igem.org/Team:NUDT_CHINA/Model/Deduction"> <img alt="300x200" src="https://static.igem.org/mediawiki/2018/d/da/T--NUDT_CHINA--our_work_you6.png"> </a>
 
</div>
 
</div>
 
</div>
 
</div>
 
                         </div>
 
                         </div>
 
                 </div>
 
                 </div>
−
<header class="section-header">
+
−
<h3>More details</h3>
+
−
</header>
+
−
<div class="col-md-12 column" alt="problem_answer">
+
−
<div class="panel-group" id="panel-1">
+
−
<div class="panel panel-success">
+
−
<div class="panel-heading text-center">
+
−
<a class="panel-title collapsed " data-toggle="collapse" data-parent="#panel-1" href="#panel-element-1"  style="color: #079209; font-size: 20px;text-decoration:none;">Question 1: What does the structure of domains look like?</a>
+
−
</div>
+
−
<div id="panel-element-1" class="panel-collapse collapse">
+
−
<div class="panel-body" style="font-size:16px;">
+
−
There are several variants of  derived from different organisms. In our project, we  . The structure of this protein has been determined with and without  The  a  lobe. The two lobes are positively charged towards the protein core to accommodate the negatively charged RNA. Each of these two lobes contains an RNase domain. At the  main is responsible for target cleavage. In contrast to other  two RNase domains of the NUC lobe are located at the outside of the protein, which is likely the reason collateral cleavage upon activation by binding to a matching target. These two domains have been labeled as red spots in Figure 2 and can be found at the interface between the green and pink domain.
+
−
</div>
+
−
</div>
+
−
</div>
+
−
<div class="panel panel-success">
+
−
<div class="panel-heading text-center">
+
−
<a class="panel-title collapsed" data-toggle="collapse" data-parent="#panel-1" href="#panel-element-2" style="color: #079209; font-size: 20px;text-decoration:none;">Collapsible Group Item #2</a>
+
−
</div>
+
−
<div id="panel-element-2" class="panel-collapse collapse">
+
−
<div class="panel-body" style="font-size:16px;">
+
−
Anim pariatur cliche...
+
−
</div>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
</div>
+
  
  
 
</html>
 
</html>
 
{{NUDT_CHINA/footer}}
 
{{NUDT_CHINA/footer}}

Latest revision as of 02:00, 18 October 2018

Designed Protein Degradation Method Based on

Trim21 And Nanobody              -- Model

Our Work


Our innovative project present a target protein disruption method in order to degrade endogenous target proteins acutely in mammalian cells.

To explain and predict the possibilities within the wetlab limits,we built ODE models and applied our measured data to parameters simulation method based on Bayesian parameter estimation to obtain parameters which fits our reality better.

We use this model to obtain the relation between the expression time of plasmid and protein degradation rate,making it accessible to future teams who want to incorporate our minds into their designs.

Through the modeling ,we understand the reaction mechanism more specifically and obtain how different factors influence the degradation process .This model also offers guidelines on choosing detection point and future clinical application.

300x200

Basic Work

GFP

ErbB3

300x200
300x200