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Revision as of 02:30, 18 October 2018

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Parts

Parts

TokyoTech 2018 iGEM Team Parts

NameTypeDescriptionDesignLength(bp)
BBa_K2729000CodingDENV1 CHayato Ito, Kotaro Miyamoto, Hajime Fujita300
BBa_K2729001CodingDENV2 CHayato Ito, Kotaro Miyamoto, Hajime Fujita300
BBa_K2729002CodingDENV3 CHayato Ito, Kotaro Miyamoto, Hajime Fujita300
BBa_K2729003CodingDENV4 CHayato Ito, Kotaro Miyamoto, Hajime Fujita300
BBa_K2729004CodingDENV3 EHayato Ito, Kotaro Miyamoto, Hajime Fujita1479
BBa_K2729005CodingDENV4 EHayato Ito, Kotaro Miyamoto, Hajime Fujita1485
BBa_K2729006CodingEGFP_FMDV2aHayato Ito, Kotaro Miyamoto, Hajime Fujita950
BBa_K2729007CodingDsRed_FMDV2aHayato Ito, Kotaro Miyamoto, Hajime Fujita908
BBa_K2729008CodingZsYellow_FMDV2aHayato Ito, Kotaro Miyamoto, Hajime Fujita920
BBa_K2729009CodingAmCyan_FMDV2aHayato Ito, Kotaro Miyamoto, Hajime Fujita920
BBa_K2729010CodingDENV1 AncC_prM_EHayato Ito, Kotaro Miyamoto, Hajime Fujita2028
BBa_K2729011CodingDENV2 AncC_prM_EHayato Ito, Kotaro Miyamoto, Hajime Fujita2028
BBa_K2729012CodingDENV3 AncC_prM_EHayato Ito, Kotaro Miyamoto, Hajime Fujita2022
BBa_K2729013CodingDENV4 AncC_prM_EHayato Ito, Kotaro Miyamoto, Hajime Fujita2028

Best Parts: EGFP-FMDV2a (BBa_K2729006)

Our team made new pseudovirus that can infect Vero cells and introduce the gene including this EGFP-FMDV2a. With the assistance of "self-cleaving" 2A peptides, EGFP is produced inside of the cell and folded so that it can emit fluorescence at the end.

Since EGFP is 35 times brighter than wild-type GFP and FMDV2a precisely works in our assumption, you can easily check the degree of infection by measuring fluorescence intensity.


Biosafety

As you can see in Fig. 9, on native virus genome, structural genes and non-structural genes are integrated on the same sequence. Thus, the whole genome are replicated and that make viruses possible to replicate themselves in host cells. However, as you can see our construct design in Fig. 10, structural and non-structural regions are splitted so that the pseudo-virus cannot replicate themselves in host cells because RNA polymerase from non-structural V gene cannot replicate structural genes. To sum up, our system starts and ends in one place and doesn't harm environment.

Address

2 Chome-12-1
Ookayama, Meguro, Tokyo

Contacts

Email: igem2018tokyotech@gmail.com

Links

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