Difference between revisions of "Team:Toronto/WetLab"

 
(27 intermediate revisions by 4 users not shown)
Line 2: Line 2:
 
<html>
 
<html>
 
<style>
 
<style>
−
.teamBanner{
+
.textArial{
−
position: relative;
+
font-family: 'Arial Black', Gadget, sans-serif;  
−
text-align: center;
+
font-size: 40px;
−
padding-top:100px;
+
line-height: 40px;  
−
padding-bottom:100px;
+
−
list-style: none;
+
−
font-family: "Impact", "Charcoal", sans-serif;
+
−
margin-top: 0px;
+
−
font-size:44px;
+
−
width:100%;
+
−
background-color:#0B75AF;
+
−
color:#FFF;
+
−
background-image: linear-gradient(to right, black , yellow);
+
 
}
 
}
−
.teamBanner .content{
+
 
−
position: absolute;
+
.textArial a {
−
z-index:5;
+
    color: white;
−
text-align: center;
+
    text-decoration: none;
−
width: 100%;
+
−
}
+
−
.teamBanner::after{
+
−
content: "";
+
−
  position: absolute;
+
−
  top: 0px;
+
−
  left: 0px;
+
−
  width: 100%;
+
−
  height: 100%;
+
−
  z-index:6;
+
−
  background-image: url(https://i.stack.imgur.com/Hp53k.jpg);
+
−
  background-size: cover;
+
−
  opacity: 0.2;
+
 
}
 
}
  
−
.blackBackround{
+
#bodyContent h1, #bodyContent h2{
−
background-color: #000;
+
  margin-bottom: 0px;
 
}
 
}
 +
 
</style>
 
</style>
  
 
<body>
 
<body>
−
<div class="blackBackround">
+
<div style="width:100%;color:white;text-align: center;padding-top:550px;padding-bottom:0px;background-image:url('https://static.igem.org/mediawiki/2018/0/0a/T--Toronto--wetbanner.jpg'); background-color:rgba(0,0,0,0.0); background-repeat: no-repeat; background-size: cover;">
−
<h1 class="teamBanner">Wet Lab</h1>  
+
<div style="width:100%; margin:0 auto; background-color:rgba(0,0,0,0.7); padding-top: 30px; padding-bottom: 50px">
 +
<h1 style="background-color:rgba(0,0,0,0.0); color:white;font: bold 40px/80px Helvetica, Sans-Serif; -webkit-text-stroke-width: 2px; -webkit-text-stroke-color: black; font-size: 90px;">WetLab</h1>
 +
</div>
 +
</div>
 +
<div style="background-color: rgba(15,65,92,1.0); background-image: linear-gradient(#3b1919, #000000);">
 +
<div class="center" style="height: 400px; width: 80%;">
 +
<div class="centerPacker">
 +
  <p style="color: rgba(255,255,255,1.0); font-size: 20px; line-height:20px; text-align: center;">The wet lab team sought to establish an optimised protocol that would confer floatation to <i>Escherichia coli</i> when transformed with plasmid containing a gas vesicle proteins operon. This plasmid is a synthetic hybrid of gas vesicle proteins from wild <i>Aphanizomenon flos-aquae</i> and <i>Bacillus megaterium</i>. We were able to use empirical data from growth curves of our experimental cell lines and use it as a parameter to model our floating cells in the context of a bioreactor computer model.</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div style="background-color: rgba(0,0,0,1.0);">
 +
<div class="center" style="height: 550px; width: 80%;">
 +
<div class="centerPacker">
 +
<div class="grid-container" style="padding-left: 100px; padding-right:100px; margin-left: auto; margin-right: auto; grid-template-columns: 300px 300px 300px 300px 300px">
 +
  <div class="item1">
 +
<h1 class="textArial" style="text-align: center;"><a href="https://2018.igem.org/Team:Toronto/WetLab/ExperimentProtocols">
 +
<img src="https://static.igem.org/mediawiki/2018/1/11/T--Toronto--chemical_reaction.svg" alt="icon1" style="width:200px;"> </br>
 +
Protocols </a></h1>
 
</div>
 
</div>
−
<div>
+
  <div class="item2">
−
<h1>Dry Lab</h1>
+
<h1 class="textArial" style="text-align: center;"><a href="https://2018.igem.org/Team:Toronto/WetLab/Notebook">
−
<h1>Content</h1>
+
<img src="https://static.igem.org/mediawiki/2018/1/17/T--Toronto--notepad.svg" alt="icon1" style="width:200px;"> </br>
−
<h1>Dry Lab</h1>
+
Notebook</a></h1>
−
<h1>Content</h1>
+
</div>  
−
<h1>Dry Lab</h1>  
+
<div class="item3">
−
<h1>Content</h1>
+
<h1 class="textArial" style="text-align: center;"><a href="https://2018.igem.org/Team:Toronto/WetLab/Results">
−
<h1>Dry Lab</h1>
+
<img src="https://static.igem.org/mediawiki/2018/b/b6/T--Toronto--m-biology.svg" alt="icon1" style="width:200px;"> </br>
−
<h1>Content</h1>
+
Results</a></h1>
−
<h1>Dry Lab</h1>
+
−
<h1>Content</h1>  
+
−
<h1>Dry Lab</h1>  
+
−
<h1>Content</h1>  
+
−
<h1>Dry Lab</h1>  
+
−
<h1>Content</h1>  
+
−
<h1>Dry Lab</h1>  
+
−
<h1>Content</h1>  
+
−
<h1>Dry Lab</h1>  
+
−
<h1>Content</h1>  
+
−
<h1>Dry Lab</h1>
+
−
<h1>Content</h1>  
+
 
</div>
 
</div>
 +
  <div class="item4">
 +
    <h1 class="textArial" style="text-align: center;"><a href="https://2018.igem.org/Team:Toronto/WetLab/Parts">
 +
      <img src="https://static.igem.org/mediawiki/2018/e/eb/T--Toronto--circuit.svg" alt="icon1" style="width:200px;"> </br>
 +
      Parts </a></h1>
 +
  </div>
 +
  <div class="item5">
 +
    <h1 class="textArial" style="text-align: center;"><a href="https://2018.igem.org/Team:Toronto/InterLab">
 +
      <img src="https://static.igem.org/mediawiki/2018/f/f4/T--Toronto--test-tubes.svg" alt="icon1" style="width:200px;"> </br>
 +
      Interlab</a></h1>
 +
  </div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 03:48, 18 October 2018

WetLab

The wet lab team sought to establish an optimised protocol that would confer floatation to Escherichia coli when transformed with plasmid containing a gas vesicle proteins operon. This plasmid is a synthetic hybrid of gas vesicle proteins from wild Aphanizomenon flos-aquae and Bacillus megaterium. We were able to use empirical data from growth curves of our experimental cell lines and use it as a parameter to model our floating cells in the context of a bioreactor computer model.