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− | <div style="width:100%;color:white;text-align: center;padding-top:550px;padding-bottom:0px;background-image:url('https://static.igem.org/mediawiki/2018/ | + | <div style="width:100%;color:white;text-align: center;padding-top:550px;padding-bottom:0px;background-image:url('https://static.igem.org/mediawiki/2018/0/0a/T--Toronto--wetbanner.jpg'); background-color:rgba(0,0,0,0.0); background-repeat: no-repeat; background-size: cover;"> |
<div style="width:100%; margin:0 auto; background-color:rgba(0,0,0,0.7); padding-top: 30px; padding-bottom: 50px"> | <div style="width:100%; margin:0 auto; background-color:rgba(0,0,0,0.7); padding-top: 30px; padding-bottom: 50px"> | ||
<h1 style="background-color:rgba(0,0,0,0.0); color:white;font: bold 40px/80px Helvetica, Sans-Serif; -webkit-text-stroke-width: 2px; -webkit-text-stroke-color: black; font-size: 90px;">WetLab</h1> | <h1 style="background-color:rgba(0,0,0,0.0); color:white;font: bold 40px/80px Helvetica, Sans-Serif; -webkit-text-stroke-width: 2px; -webkit-text-stroke-color: black; font-size: 90px;">WetLab</h1> | ||
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− | <div class="grid-container" style="padding-left: 100px; padding-right:100px; margin-left: auto; margin-right: auto;"> | + | <div style="background-color: rgba(15,65,92,1.0); background-image: linear-gradient(#3b1919, #000000);"> |
+ | <div class="center" style="height: 400px; width: 80%;"> | ||
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+ | <p style="color: rgba(255,255,255,1.0); font-size: 20px; line-height:20px; text-align: center;">The wet lab team sought to establish an optimised protocol that would confer floatation to <i>Escherichia coli</i> when transformed with plasmid containing a gas vesicle proteins operon. This plasmid is a synthetic hybrid of gas vesicle proteins from wild <i>Aphanizomenon flos-aquae</i> and <i>Bacillus megaterium</i>. We were able to use empirical data from growth curves of our experimental cell lines and use it as a parameter to model our floating cells in the context of a bioreactor computer model.</p> | ||
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+ | </div> | ||
+ | <div style="background-color: rgba(0,0,0,1.0);"> | ||
+ | <div class="center" style="height: 550px; width: 80%;"> | ||
+ | <div class="centerPacker"> | ||
+ | <div class="grid-container" style="padding-left: 100px; padding-right:100px; margin-left: auto; margin-right: auto; grid-template-columns: 300px 300px 300px 300px 300px"> | ||
<div class="item1"> | <div class="item1"> | ||
− | <h1 class="textArial" style="text-align: center;"><a | + | <h1 class="textArial" style="text-align: center;"><a href="https://2018.igem.org/Team:Toronto/WetLab/ExperimentProtocols"> |
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<img src="https://static.igem.org/mediawiki/2018/1/11/T--Toronto--chemical_reaction.svg" alt="icon1" style="width:200px;"> </br> | <img src="https://static.igem.org/mediawiki/2018/1/11/T--Toronto--chemical_reaction.svg" alt="icon1" style="width:200px;"> </br> | ||
Protocols </a></h1> | Protocols </a></h1> | ||
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− | <h1 class="textArial" style="text-align: center;"><a | + | <h1 class="textArial" style="text-align: center;"><a href="https://2018.igem.org/Team:Toronto/WetLab/Notebook"> |
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<img src="https://static.igem.org/mediawiki/2018/1/17/T--Toronto--notepad.svg" alt="icon1" style="width:200px;"> </br> | <img src="https://static.igem.org/mediawiki/2018/1/17/T--Toronto--notepad.svg" alt="icon1" style="width:200px;"> </br> | ||
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− | <h1 class="textArial" style="text-align: center;"><a | + | <h1 class="textArial" style="text-align: center;"><a href="https://2018.igem.org/Team:Toronto/WetLab/Results"> |
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<img src="https://static.igem.org/mediawiki/2018/b/b6/T--Toronto--m-biology.svg" alt="icon1" style="width:200px;"> </br> | <img src="https://static.igem.org/mediawiki/2018/b/b6/T--Toronto--m-biology.svg" alt="icon1" style="width:200px;"> </br> | ||
Results</a></h1> | Results</a></h1> | ||
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+ | <h1 class="textArial" style="text-align: center;"><a href="https://2018.igem.org/Team:Toronto/WetLab/Parts"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/e/eb/T--Toronto--circuit.svg" alt="icon1" style="width:200px;"> </br> | ||
+ | Parts </a></h1> | ||
+ | </div> | ||
+ | <div class="item5"> | ||
+ | <h1 class="textArial" style="text-align: center;"><a href="https://2018.igem.org/Team:Toronto/InterLab"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/f/f4/T--Toronto--test-tubes.svg" alt="icon1" style="width:200px;"> </br> | ||
+ | Interlab</a></h1> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
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Latest revision as of 03:48, 18 October 2018
WetLab
The wet lab team sought to establish an optimised protocol that would confer floatation to Escherichia coli when transformed with plasmid containing a gas vesicle proteins operon. This plasmid is a synthetic hybrid of gas vesicle proteins from wild Aphanizomenon flos-aquae and Bacillus megaterium. We were able to use empirical data from growth curves of our experimental cell lines and use it as a parameter to model our floating cells in the context of a bioreactor computer model.