Difference between revisions of "Team:Toronto/WetLab"

 
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<div style="width:100%;color:white;text-align: center;padding-top:550px;padding-bottom:0px;background-image:url('https://static.igem.org/mediawiki/2018/0/0a/T--Toronto--wetbanner.jpg'); background-color:rgba(0,0,0,0.0); background-repeat: no-repeat; background-size: cover;">
 
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<h1 style="background-color:rgba(0,0,0,0.0); color:white;font: bold 40px/80px Helvetica, Sans-Serif; -webkit-text-stroke-width: 2px; -webkit-text-stroke-color: black; font-size: 90px;">WetLab</h1>
 
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  <p style="color: rgba(255,255,255,1.0); font-size: 20px; line-height:20px; text-align: center;">The wet lab team sought to establish an optimised protocol that would confer floatation to <i>Escherichia coli</i> when transformed with plasmid containing a gas vesicle proteins operon. This plasmid is a synthetic hybrid of gas vesicle proteins from wild <i>Aphanizomenon flos-aquae</i> and <i>Bacillus megaterium</i>. We were able to use empirical data from growth curves of our experimental cell lines and use it as a parameter to model our floating cells in the context of a bioreactor computer model.</p>
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<img src="https://static.igem.org/mediawiki/2018/1/11/T--Toronto--chemical_reaction.svg" alt="icon1" style="width:200px;"> </br>
 
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Protocols </a></h1>
 
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How to replicate our experiments</br>
 
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The log of day to day activities
 
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Final conclusions
 
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Latest revision as of 03:48, 18 October 2018

WetLab

The wet lab team sought to establish an optimised protocol that would confer floatation to Escherichia coli when transformed with plasmid containing a gas vesicle proteins operon. This plasmid is a synthetic hybrid of gas vesicle proteins from wild Aphanizomenon flos-aquae and Bacillus megaterium. We were able to use empirical data from growth curves of our experimental cell lines and use it as a parameter to model our floating cells in the context of a bioreactor computer model.