Difference between revisions of "Team:Toronto/WetLab"
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| − | <p style="color: rgba(255,255,255,1.0); font-size: 20px; line-height:20px; text-align: center;">The wet lab team sought to establish an optimised protocol that would confer floatation to <i>Escherichia coli</i> when transformed with plasmid containing a gas vesicle proteins operon. This plasmid is a synthetic hybrid of gas vesicle proteins from wild <i>Aphanizomenon flos-aquae</i> and <i>Bacillus megaterium</i>. We were able to use empirical data from growth curves of our experimental cell lines and use it as a parameter to model our floating cells in a bioreactor model.</p> | + | <p style="color: rgba(255,255,255,1.0); font-size: 20px; line-height:20px; text-align: center;">The wet lab team sought to establish an optimised protocol that would confer floatation to <i>Escherichia coli</i> when transformed with plasmid containing a gas vesicle proteins operon. This plasmid is a synthetic hybrid of gas vesicle proteins from wild <i>Aphanizomenon flos-aquae</i> and <i>Bacillus megaterium</i>. We were able to use empirical data from growth curves of our experimental cell lines and use it as a parameter to model our floating cells in the context of a bioreactor computer model.</p> |
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Latest revision as of 03:48, 18 October 2018
WetLab
The wet lab team sought to establish an optimised protocol that would confer floatation to Escherichia coli when transformed with plasmid containing a gas vesicle proteins operon. This plasmid is a synthetic hybrid of gas vesicle proteins from wild Aphanizomenon flos-aquae and Bacillus megaterium. We were able to use empirical data from growth curves of our experimental cell lines and use it as a parameter to model our floating cells in the context of a bioreactor computer model.
Protocols 
Notebook
Results
Parts 
Interlab