Difference between revisions of "Team:NUDT CHINA/Experiments"

 
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<div class="col-md-12 column" style="width:100%;height:250px;background-size:contain;0px -100px; background-image:url(https://static.igem.org/mediawiki/2018/9/9f/T--NUDT_CHINA--banner_project_experiment.jpg);background-repeat:no-repeat;">
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  <p style="font-size:36px;margin: 0 0%;padding: 5% 0 0 10%;color: white;">Designed Protein Degradation Method Based on</p>
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<p style="font-size:36px;margin: 0 0%;padding: 0 0 0 10%;color: white;">Trim21 And Nanobody&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;--&nbsp;Experiments</p>
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                                <div class="col-md-push-1 col-md-10">
 
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      <h1 style="font-size: 40px;font-weight: 900">Our Work</h1>
 
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                                      <h2 style="font-size: 32px;margin-bottom: 10px;margin-top: 15px;">Our Work</h2>
 
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<p alt="content_add" style="font-size: 18px;">hello everyone this is the another example-content part for more imformation. Hello everyone this is the another example-content part for more imformation.Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui. </p>
 
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Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.
 
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<img alt="300x200" src="https://static.igem.org/mediawiki/2018/9/9b/2018_igem_menu_logo.svg" />
 
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<p alt="photo_detail" style="text-align: center;">This is the sign of the famous game!</p>
 
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<p alt="photo_detail" style="text-align: center;"><em>This is the sign of the famous game!</em></p>
 
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<p alt="content_add" style="font-size: 18px;">hello everyone this is the another example-content part for more imformation. Hello everyone this is the another example-content part for more imformation.Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui. </p>
 
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Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.
 
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<blockquote style="border-left-color: #18d26e;">
 
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Maybe you want to write <strong>something interesting thing</strong> here!
 
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<small>Someone famous in <cite>Source Title</cite></small>
 
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<p alt="content_add" style="font-size: 18px;">hello everyone this is the another example-content part for more imformation. Hello everyone this is the another example-content part for more imformation.Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui. </p>
 
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Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.
 
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<p alt="content_add" style="font-size: 18px;">hello everyone this is the another example-content part for more imformation. Hello everyone this is the another example-content part for more imformation.Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui. </p>
 
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Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.
 
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  <caption>The example table for show some data</caption>
 
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  <th>name</th>
 
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  <th>city</th>
 
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  <th>mail</th>
 
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  <td>Tanmay</td>
 
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  <td>Bangalore</td>
 
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  <td>560001</td>
 
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  <td>Sachin</td>
 
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  <td>Uma</td>
 
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Example block-level help text here.There are several variants of  derived from different organisms. In our project, we  . The structure of this protein has been determined with and without  The  a  lobe. The two lobes are positively charged towards the protein core to accommodate the negatively charged RNA. Each of these two lobes contains an RNase domain. At the  main is responsible for target cleavage. In contrast to other  two RNase domains of the NUC lobe are located at the outside of the protein, which is likely the reason collateral cleavage upon activation by binding to a matching target. These two domains have been labeled as red spots in Figure 2 and can be found at the interface between the green and pink domain.
 
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Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.
 
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<p><a href="https://static.igem.org/mediawiki/2018/3/32/T--NUDT_CHINA---EXPERIMENT1.pdf">Protocol for Annealing</a></p>              
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<p><a href="https://static.igem.org/mediawiki/2018/c/c8/T--NUDT_CHINA---EXPERIMENT3.pdf">Protocol for Ligation</a></p>  
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<p><a href="https://static.igem.org/mediawiki/2018/2/20/T--NUDT_CHINA--EXPERIMENT5.PDF">Protocol for CCK8 (Cell Counting kit-8) </a></p>  
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<p><a href="https://static.igem.org/mediawiki/2018/6/61/T--NUDT_CHINA--EXPERIMENT6.pdf">Protocol for Lipofectamine&reg; 2000 Transfection </a></p>  
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Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.
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<p><a href="https://static.igem.org/mediawiki/2018/3/3a/T--NUDT_CHINA--EXPERIMENT8.pdf">Protocol for Western blotting </a></p>  
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<h3>More details</h3>
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<p><a href="https://static.igem.org/mediawiki/2018/b/b7/T--NUDT_CHINA--EXPERIMENT9.pdf">Protocol for cell culture and subculture </a></p>  
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<p><a href="https://static.igem.org/mediawiki/2018/4/48/T--NUDT_CHINA--EXPERIMENT19.pdf">Protocol for PCR with PrimeStar HS DNA polymerase </a></p>                  
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<p><a href="https://static.igem.org/mediawiki/2018/a/a2/T--NUDT_CHINA--EXPERIMENT13.pdf">Protocol for Phosphorylation Modification
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<a class="panel-title collapsed " data-toggle="collapse" data-parent="#panel-1" href="#panel-element-1"  style="color: #079209; font-size: 20px;text-decoration:none;">Question 1: What does the structure of domains look like?</a>
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<p><a href="https://static.igem.org/mediawiki/2018/5/5a/T--NUDT_CHINA--EXPERIMENT20.pdf">Protocol for colony PCR </a></p>
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There are several variants of  derived from different organisms. In our project, we  . The structure of this protein has been determined with and without  The  a  lobe. The two lobes are positively charged towards the protein core to accommodate the negatively charged RNA. Each of these two lobes contains an RNase domain. At the  main is responsible for target cleavage. In contrast to other  two RNase domains of the NUC lobe are located at the outside of the protein, which is likely the reason collateral cleavage upon activation by binding to a matching target. These two domains have been labeled as red spots in Figure 2 and can be found at the interface between the green and pink domain.
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<a class="panel-title collapsed" data-toggle="collapse" data-parent="#panel-1" href="#panel-element-2" style="color: #079209; font-size: 20px;text-decoration:none;">Collapsible Group Item #2</a>
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Anim pariatur cliche...
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<p><a href="https://static.igem.org/mediawiki/2018/4/45/T--NUDT_CHINA--EXPERIMENT21.PDF">Protocol for Agarose Gel Electrophoresis </a></p>
  
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<p><a href="https://static.igem.org/mediawiki/2018/1/1e/T--NUDT_CHINA--EXPERIMENT22.PDF">Protocol for double digestion </a></p> 
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<p><a href="https://static.igem.org/mediawiki/2018/4/47/T--NUDT_CHINA--EXPERIMENT23.PDF">Protocol for Gel Purification with OMEGA Gel Extraction Kit </a></p> 
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<p><a href="https://static.igem.org/mediawiki/2018/a/a7/T--NUDT_CHINA---EXPERIMENT25.pdf">Protocol for mini-prep with OMEGA E.Z.N.A.&reg; Plasmid DNA Mini Kit I </a></p> 
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{{NUDT_CHINA/footer}}
 
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Latest revision as of 03:53, 18 October 2018

Designed Protein Degradation Method Based on

Trim21 And Nanobody              -- Experiments

Protocol for Annealing

Protocol for Ligation

Protocol for CCK8 (Cell Counting kit-8)

Protocol for Lipofectamine® 2000 Transfection

Protocol for Western blotting

Protocol for cell culture and subculture

Protocol for PCR with PrimeStar HS DNA polymerase

Protocol for Phosphorylation Modification

Protocol for colony PCR

Protocol for Agarose Gel Electrophoresis

Protocol for double digestion

Protocol for Gel Purification with OMEGA Gel Extraction Kit

Protocol for mini-prep with OMEGA E.Z.N.A.® Plasmid DNA Mini Kit I