Difference between revisions of "Team:NUDT CHINA/Experiments"

 
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<p><a href="https://static.igem.org/mediawiki/2018/3/32/T--NUDT_CHINA---EXPERIMENT1.pdf">Protocol for Annealing</a></p>             
  
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<p><a href="https://static.igem.org/mediawiki/2018/c/c8/T--NUDT_CHINA---EXPERIMENT3.pdf">Protocol for Ligation</a></p>
  
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<p><a href="https://static.igem.org/mediawiki/2018/2/20/T--NUDT_CHINA--EXPERIMENT5.PDF">Protocol for CCK8 (Cell Counting kit-8) </a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/6/61/T--NUDT_CHINA--EXPERIMENT6.pdf">Protocol for Lipofectamine&reg; 2000 Transfection </a></p>
 
 
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<p><a href="https://static.igem.org/mediawiki/2018/3/3a/T--NUDT_CHINA--EXPERIMENT8.pdf">Protocol for Western blotting </a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/b/b7/T--NUDT_CHINA--EXPERIMENT9.pdf">Protocol for cell culture and subculture </a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/4/48/T--NUDT_CHINA--EXPERIMENT19.pdf">Protocol for PCR with PrimeStar HS DNA polymerase </a></p>                 
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<p><a href="https://static.igem.org/mediawiki/2018/a/a2/T--NUDT_CHINA--EXPERIMENT13.pdf">Protocol for Phosphorylation Modification
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<p><a href="https://static.igem.org/mediawiki/2018/5/5a/T--NUDT_CHINA--EXPERIMENT20.pdf">Protocol for colony PCR </a></p> 
  
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<p><a href="https://static.igem.org/mediawiki/2018/4/45/T--NUDT_CHINA--EXPERIMENT21.PDF">Protocol for Agarose Gel Electrophoresis </a></p>  
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<h3>More details</h3>
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<a class="panel-title collapsed " data-toggle="collapse" data-parent="#panel-1" href="#panel-element-1"  style="color: #079209; font-size: 20px;text-decoration:none;">Question 1: What does the structure of domains look like?</a>
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There are several variants of  derived from different organisms. In our project, we  . The structure of this protein has been determined with and without  The  a  lobe. The two lobes are positively charged towards the protein core to accommodate the negatively charged RNA. Each of these two lobes contains an RNase domain. At the  main is responsible for target cleavage. In contrast to other  two RNase domains of the NUC lobe are located at the outside of the protein, which is likely the reason collateral cleavage upon activation by binding to a matching target. These two domains have been labeled as red spots in Figure 2 and can be found at the interface between the green and pink domain.
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<a class="panel-title collapsed" data-toggle="collapse" data-parent="#panel-1" href="#panel-element-2" style="color: #079209; font-size: 20px;text-decoration:none;">Collapsible Group Item #2</a>
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<p><a href="https://static.igem.org/mediawiki/2018/1/1e/T--NUDT_CHINA--EXPERIMENT22.PDF">Protocol for double digestion </a></p> 
  
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<p><a href="https://static.igem.org/mediawiki/2018/4/47/T--NUDT_CHINA--EXPERIMENT23.PDF">Protocol for Gel Purification with OMEGA Gel Extraction Kit </a></p> 
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<p><a href="https://static.igem.org/mediawiki/2018/a/a7/T--NUDT_CHINA---EXPERIMENT25.pdf">Protocol for mini-prep with OMEGA E.Z.N.A.&reg; Plasmid DNA Mini Kit I </a></p> 
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Latest revision as of 03:53, 18 October 2018

Designed Protein Degradation Method Based on

Trim21 And Nanobody              -- Experiments

Protocol for Annealing

Protocol for Ligation

Protocol for CCK8 (Cell Counting kit-8)

Protocol for Lipofectamine® 2000 Transfection

Protocol for Western blotting

Protocol for cell culture and subculture

Protocol for PCR with PrimeStar HS DNA polymerase

Protocol for Phosphorylation Modification

Protocol for colony PCR

Protocol for Agarose Gel Electrophoresis

Protocol for double digestion

Protocol for Gel Purification with OMEGA Gel Extraction Kit

Protocol for mini-prep with OMEGA E.Z.N.A.® Plasmid DNA Mini Kit I