Difference between revisions of "Team:NUDT CHINA/Experiments"

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<div class="col-md-12 column" style="width:100%;height:250px;background-size:contain;0px -100px; background-image:url(https://static.igem.org/mediawiki/2018/9/9f/T--NUDT_CHINA--banner_project_experiment.jpg);background-repeat:no-repeat;">
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  <p style="font-size:36px;margin: 0 0%;padding: 5% 0 0 10%;color: white;">Designed Protein Degradation Method Based on</p>
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<p style="font-size:36px;margin: 0 0%;padding: 0 0 0 10%;color: white;">Trim21 And Nanobody&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;--&nbsp;Experiments</p>
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<h1>Experiments</h1>
 
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<p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
 
  
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Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.
 
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<p><a href="https://static.igem.org/mediawiki/2018/3/32/T--NUDT_CHINA---EXPERIMENT1.pdf">Protocol for Annealing</a></p>              
  
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<p><a href="https://static.igem.org/mediawiki/2018/c/c8/T--NUDT_CHINA---EXPERIMENT3.pdf">Protocol for Ligation</a></p>
  
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<p><a href="https://static.igem.org/mediawiki/2018/2/20/T--NUDT_CHINA--EXPERIMENT5.PDF">Protocol for CCK8 (Cell Counting kit-8) </a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/6/61/T--NUDT_CHINA--EXPERIMENT6.pdf">Protocol for Lipofectamine&reg; 2000 Transfection </a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/3/3a/T--NUDT_CHINA--EXPERIMENT8.pdf">Protocol for Western blotting </a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/b/b7/T--NUDT_CHINA--EXPERIMENT9.pdf">Protocol for cell culture and subculture </a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/4/48/T--NUDT_CHINA--EXPERIMENT19.pdf">Protocol for PCR with PrimeStar HS DNA polymerase </a></p>                 
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<p><a href="https://static.igem.org/mediawiki/2018/a/a2/T--NUDT_CHINA--EXPERIMENT13.pdf">Protocol for Phosphorylation Modification
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</a></p>                 
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<p><a href="https://static.igem.org/mediawiki/2018/5/5a/T--NUDT_CHINA--EXPERIMENT20.pdf">Protocol for colony PCR </a></p> 
  
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<p><a href="https://static.igem.org/mediawiki/2018/4/45/T--NUDT_CHINA--EXPERIMENT21.PDF">Protocol for Agarose Gel Electrophoresis </a></p>  
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<h3>What should this page contain?</h3>
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<ul>
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<li> Protocols </li>
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<li> Experiments </li>
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<li> Documentation of the development of your project </li>
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<h3>Inspiration</h3>
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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<p><a href="https://static.igem.org/mediawiki/2018/1/1e/T--NUDT_CHINA--EXPERIMENT22.PDF">Protocol for double digestion </a></p>
  
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<p><a href="https://static.igem.org/mediawiki/2018/4/47/T--NUDT_CHINA--EXPERIMENT23.PDF">Protocol for Gel Purification with OMEGA Gel Extraction Kit </a></p> 
  
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<p><a href="https://static.igem.org/mediawiki/2018/a/a7/T--NUDT_CHINA---EXPERIMENT25.pdf">Protocol for mini-prep with OMEGA E.Z.N.A.&reg; Plasmid DNA Mini Kit I </a></p> 
  
  
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Latest revision as of 03:53, 18 October 2018

Designed Protein Degradation Method Based on

Trim21 And Nanobody              -- Experiments

Protocol for Annealing

Protocol for Ligation

Protocol for CCK8 (Cell Counting kit-8)

Protocol for Lipofectamine® 2000 Transfection

Protocol for Western blotting

Protocol for cell culture and subculture

Protocol for PCR with PrimeStar HS DNA polymerase

Protocol for Phosphorylation Modification

Protocol for colony PCR

Protocol for Agarose Gel Electrophoresis

Protocol for double digestion

Protocol for Gel Purification with OMEGA Gel Extraction Kit

Protocol for mini-prep with OMEGA E.Z.N.A.® Plasmid DNA Mini Kit I