Difference between revisions of "Team:William and Mary/Attributions"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1 style="color: black;text-align:center;">Attributions</h1>
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<div style='padding-top: 40px;'></div>
  
  
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 50px;line-height: 25px;font-size: 18px;' >
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The William and Mary iGEM team is deeply grateful to all who supported the team throughout the year. Behind our team is a community of supporters, whether advisors, friends, or sponsors, and our project would not have been possible without them.
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</div>
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<div style='padding-top: 40px;'></div>
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<div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 25px' ><b>Advisors</b></div>
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<div style='background: #808080; margin: 0px 14% 20px 14%; height:1px;></div>
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 50px;line-height: 25px;font-size: 18px;' >
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We would like to thank Dr. Margaret Saha for the instrumental role she played in our project. As our PI and team advisor, she has poured a great amount of energy into ensuring our team has the tools we need to succeed. Her dedication and boundless enthusiasm have been invaluable and for this we are forever grateful. We would also like to thank Dr. Patel for his guidance with our math modeling; he has gone above and beyond in mentoring the math team.</div>
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<div style='padding-top: 40px;'></div>
  
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<div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 25px' ><b>Other Acknowledgements</b></div>
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<h1>Attributions</h1>
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We’d like to thank Dr. Eric Bradley for his assistance with lab facilities, Dr. Mark Forsyth, and Dr. Joshua Puzey. We’d also like to thank Jacob Sawyer, advanced imaging specialist, for his microscopy guidance.</div>
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<p>This page is your opportunity to explain what parts of your project you did and what was done by technicians, advisers, etc. This requirement is not about literature references - these can and should be displayed throughout your wiki.
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</p>
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<h3> Bronze Medal Criterion #3</h3>
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 50px;line-height: 25px;font-size: 18px;' >
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<p> All of the work done in your project must be attributed correctly on this page. You must clearly state the work that was done by the students on your team and note any work that was done by people outside of your team, including the host labs, advisors, instructors, and individuals not on the team roster.  
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We would also like to thank John Marken our student advisor for his assistance in developing the conceptual framework of our project, as well as for his mentoring of our math team and for his invaluable advice on the presentation of our project.
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Please see the <a href="https://2018.igem.org/Judging/Medals">Medals requirements page</a> for more details.</p>
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 50px;line-height: 25px;font-size: 18px;' >
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Special thanks to Andy Halleran of Richard Murray Lab at the California Institute of Technology for his technical support and for providing us with a starter set of 3G compatible parts as well as protocols and advice on using them.</div>
  
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<div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 25px' ><b>Human Practices Support</b></div>
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<h3> What should this page contain?</h3>
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Our outreach efforts would not have been possible without support from the following: </div><br>
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 0px;line-height: 25px;'>
 
<ul>
 
<ul>
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<li>Clearly state what the team accomplished</li>
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<li>Anne Petersen: Science Coordinator for the Virginia Department of Education</li>
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<li>General Support</li>
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<li>Jennifer Daugherty: Regional Sales Manager for Thermofisher Scientific </li>
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<li>Project support and advice</li>
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<li>Donna Farnham: Counsel Event Assistant for Girl Scouts of the Colonial Coast.</li>
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<li>Fundraising help and advice</li>
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<li>Jacqueline Brooks: Counsel Partnership and Event Manager Girl Scouts of the Colonial Coast</li>
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<li>Lab support</li>
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<li>Ashley Zhang: Under Secretary General of the General Assembly William and Mary Model United Nations</li>
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<li>Difficult technique support</li>
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<li>Laura Mullen: Manager of Activitie,s Williamsburg Landing</li>
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<li>Project advisor support</li>
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<li>John Leone: Williamsburg-James City Public School Educator</li>
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<li>Wiki support</li>
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<li>Deanna Marroletti: Camp Launch</li>
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<li>Presentation coaching</li>
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<li>Surgeon Singh: Spira, CTO (business speaker)</li>
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<li>Human Practices support</li>
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<li>Amanda Synder: Medical Explorers</li>
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
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<li>Patel Ramsey: WindsorMeade, Lifestyle Coordinator</li>
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</ul>
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<li>Valerie Cushman: Women’s Weekend, Director of Alumni Initiatives William and Mary</li>
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</div>
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<li>Graham Henshaw: Clinical professor at the William and Mary Mason School of Business, Executive Director of the Entrepreneurship Center</li>
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<li>NASA: Mini-proposal (funding)</li>
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<li>William and Mary STEMinist Club</li>
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<li>Williamsburg Classical Academy (homeschooling coalition)</li>
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<li>Building with Biology</li>
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<li>Williamsburg Gazette</li>
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<li>Melissa Degaraff - Administrative Coordinator Christopher Wren Association</li>
  
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
 
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<ul>
 
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<li>Does your institution teach an iGEM or synthetic biology course?</li>
 
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<li>When did you start this course?</li>
 
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<li>Are the syllabus and course materials freely available online?</li>
 
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<li>When did you start your brainstorming?</li>
 
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<li>When did you start in the lab?</li>
 
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<li>When did you start working on  your project?</li>
 
 
</ul>
 
</ul>
 
</div>
 
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<div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 25px' ><b>Funding</b></div>
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<div style='padding-top: 0px;'></div>
  
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<h3>Inspiration</h3>
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 50px;line-height: 25px;font-size: 18px;' >
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<p>Take a look at what other teams have done:</p>
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Dr. Dennis Manos has been an invaluable resource in providing financial support. We would also like to thank the following organizations for generously providing supplies and funding to help our ideas become a reality:</div>
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 0px;line-height: 25px;'>
 
<ul>
 
<ul>
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
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<li>Epoch Life Sciences Inc</li>
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<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
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<li>BioBasic</li>
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<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
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<li>NEB</li>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
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</ul>
 
</ul>
 
</div>
 
</div>
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</div>
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<div style='padding-top: 40px;'></div>
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<div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 25px' ><b>Statement of work</b></div>
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<div style='padding-top: 0px;'></div>
  
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 50px;line-height: 25px;font-size: 18px;' >
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All work was performed by the undergraduate members of unless indicated otherwise. Our three high school trainees (Nitin Arora, Nikhil Arora and Davis Hatcher) helped us with some of our molecular cloning work in July (Colony PCRs, Gel electrophoresis) as a hands on experience to help them get acquainted with molecular cloning techniques while they designed their projects.
  
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All text, figures code, and experiments were conceived, designed and implemented by the team, with advising from our PIs and our student advisors.
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<div style = 'padding-left: 14%; padding-bottom: 10px;font-size: 25px' ><b>Reference List</b></div>
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<h3> Why is this page needed? </h3>
 
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
 
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<p>
 
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
 
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 0px;line-height: 25px;font-size: 18px;' >Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M. Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074
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<div class="highlight decoration_B_full">
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<h3> Can we base our project on a previous one? </h3>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 0px;line-height: 25px;font-size: 18px;' >D Ewen Cameron and James J Collins. Tunable protein degradation in bacteria. Nature biotechnology, 32(12):1276–1281, 20</div>
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<div style='padding-top: 5px;'></div>
  
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 0px;line-height: 25px;font-size: 18px;' >Gal-Mor O, Valdez Y, Finlay BB. The temperature-sensing protein TlpA is repressed by PhoP and dispensable for virulence of Salmonella enterica serovar Typhimurium in mice. Microbes Infect / Institut Pasteur. 2006;8:2154–62. doi:10.1016/j.micinf.2006.04.015. </div>
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<div style='padding-top: 5px;'></div>
  
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 0px;line-height: 25px;font-size: 18px;' >CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. Sonya V. Iverson, Traci L. Haddock, Jacob Beal, and Douglas M. Densmore. ACS Synthetic Biology 2016 5 (1), 99-103 DOI: 10.1021/acssynbio.5b00124</div>
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<div style='padding-top: 5px;'></div>
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 0px;line-height: 25px;font-size: 18px;' >Kracikova, M., Akiri, G., George, A., Sachidanandam, R., and Aaronson, S.A. (2013). A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis. Cell Death Differ. 20, 576–588.</div>
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<div style='padding-top: 5px;'></div>
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 0px;line-height: 25px;font-size: 18px;' >Nandan Kumar Jana, Siddhartha Roy, Bhabatarak Bhattacharyya, Nitai Chandra Mandal; Amino acid changes in the repressor of bacteriophage lambda due to temperature-sensitive mutations in its cI gene and the structure of a highly temperature-sensitive mutant repressor, Protein Engineering, Design and Selection, Volume 12, Issue 3, 1 March 1999, Pages 225–233, https://doi.org/10.1093/protein/12.3.225</div>
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<div style='padding-top: 5px;'></div>
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 0px;line-height: 25px;font-size: 18px;' >One-Step Cloning and Chromosomal Integration of DNA
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François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin. ACS Synthetic Biology 2013 2 (9), 537-541. DOI: 10.1021/sb400021</div>
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<div style='padding-top: 5px;'></div>
 +
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 0px;line-height: 25px;font-size: 18px;' >Sussman, R. & Jacob, F. Sur un systeme de repression thermosensible chez le bacteriophage lambda d’Escherichia coli. C. R. Hebd. Seances Acad. Sci. 24, 1517–1519 (1962).</div>
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<div style='padding-top: 5px;'></div>
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<div style = 'padding-right: 14%; padding-left: 14%; text-indent: 0px;line-height: 25px;font-size: 18px;' >Zhang C, Tsoi R, Wu F, You L (2016) Processing Oscillatory Signals by Incoherent Feedforward Loops. PLoS Comput Biol 12(9): e1005101. doi:10.1371/journal.pcbi.1005101</div>
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{{William_and_Mary/FOOTER}}

Latest revision as of 03:56, 18 October 2018

Attributions

The William and Mary iGEM team is deeply grateful to all who supported the team throughout the year. Behind our team is a community of supporters, whether advisors, friends, or sponsors, and our project would not have been possible without them.
Advisors
We would like to thank Dr. Margaret Saha for the instrumental role she played in our project. As our PI and team advisor, she has poured a great amount of energy into ensuring our team has the tools we need to succeed. Her dedication and boundless enthusiasm have been invaluable and for this we are forever grateful. We would also like to thank Dr. Patel for his guidance with our math modeling; he has gone above and beyond in mentoring the math team.
Other Acknowledgements
We’d like to thank Dr. Eric Bradley for his assistance with lab facilities, Dr. Mark Forsyth, and Dr. Joshua Puzey. We’d also like to thank Jacob Sawyer, advanced imaging specialist, for his microscopy guidance.
We would also like to thank John Marken our student advisor for his assistance in developing the conceptual framework of our project, as well as for his mentoring of our math team and for his invaluable advice on the presentation of our project.
Special thanks to Andy Halleran of Richard Murray Lab at the California Institute of Technology for his technical support and for providing us with a starter set of 3G compatible parts as well as protocols and advice on using them.
Human Practices Support
Our outreach efforts would not have been possible without support from the following:

  • Anne Petersen: Science Coordinator for the Virginia Department of Education
  • Jennifer Daugherty: Regional Sales Manager for Thermofisher Scientific
  • Donna Farnham: Counsel Event Assistant for Girl Scouts of the Colonial Coast.
  • Jacqueline Brooks: Counsel Partnership and Event Manager Girl Scouts of the Colonial Coast
  • Ashley Zhang: Under Secretary General of the General Assembly William and Mary Model United Nations
  • Laura Mullen: Manager of Activitie,s Williamsburg Landing
  • John Leone: Williamsburg-James City Public School Educator
  • Deanna Marroletti: Camp Launch
  • Surgeon Singh: Spira, CTO (business speaker)
  • Amanda Synder: Medical Explorers
  • Patel Ramsey: WindsorMeade, Lifestyle Coordinator
  • Valerie Cushman: Women’s Weekend, Director of Alumni Initiatives William and Mary
  • Graham Henshaw: Clinical professor at the William and Mary Mason School of Business, Executive Director of the Entrepreneurship Center
  • NASA: Mini-proposal (funding)
  • William and Mary STEMinist Club
  • Williamsburg Classical Academy (homeschooling coalition)
  • Building with Biology
  • Williamsburg Gazette
  • Melissa Degaraff - Administrative Coordinator Christopher Wren Association
Funding
Dr. Dennis Manos has been an invaluable resource in providing financial support. We would also like to thank the following organizations for generously providing supplies and funding to help our ideas become a reality:
  • Epoch Life Sciences Inc
  • BioBasic
  • NEB
Statement of work
All work was performed by the undergraduate members of unless indicated otherwise. Our three high school trainees (Nitin Arora, Nikhil Arora and Davis Hatcher) helped us with some of our molecular cloning work in July (Colony PCRs, Gel electrophoresis) as a hands on experience to help them get acquainted with molecular cloning techniques while they designed their projects. All text, figures code, and experiments were conceived, designed and implemented by the team, with advising from our PIs and our student advisors.
Reference List
Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M. Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074
D Ewen Cameron and James J Collins. Tunable protein degradation in bacteria. Nature biotechnology, 32(12):1276–1281, 20
Gal-Mor O, Valdez Y, Finlay BB. The temperature-sensing protein TlpA is repressed by PhoP and dispensable for virulence of Salmonella enterica serovar Typhimurium in mice. Microbes Infect / Institut Pasteur. 2006;8:2154–62. doi:10.1016/j.micinf.2006.04.015.
CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. Sonya V. Iverson, Traci L. Haddock, Jacob Beal, and Douglas M. Densmore. ACS Synthetic Biology 2016 5 (1), 99-103 DOI: 10.1021/acssynbio.5b00124
Kracikova, M., Akiri, G., George, A., Sachidanandam, R., and Aaronson, S.A. (2013). A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis. Cell Death Differ. 20, 576–588.
Nandan Kumar Jana, Siddhartha Roy, Bhabatarak Bhattacharyya, Nitai Chandra Mandal; Amino acid changes in the repressor of bacteriophage lambda due to temperature-sensitive mutations in its cI gene and the structure of a highly temperature-sensitive mutant repressor, Protein Engineering, Design and Selection, Volume 12, Issue 3, 1 March 1999, Pages 225–233, https://doi.org/10.1093/protein/12.3.225
One-Step Cloning and Chromosomal Integration of DNA François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin. ACS Synthetic Biology 2013 2 (9), 537-541. DOI: 10.1021/sb400021
Sussman, R. & Jacob, F. Sur un systeme de repression thermosensible chez le bacteriophage lambda d’Escherichia coli. C. R. Hebd. Seances Acad. Sci. 24, 1517–1519 (1962).
Zhang C, Tsoi R, Wu F, You L (2016) Processing Oscillatory Signals by Incoherent Feedforward Loops. PLoS Comput Biol 12(9): e1005101. doi:10.1371/journal.pcbi.1005101