Difference between revisions of "Team:Waterloo/Meet the Microbes"

 
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<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Experiments"><span>Experiments</span></a></li>
 
<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Experiments"><span>Experiments</span></a></li>
 
</li>
 
</li>
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<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Turbidostat"><span>Turbidostat</span></a></li>
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<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Interlab"><span>Interlab</span></a></li>
 
</li>
 
</li>
 
<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Demonstrate"><span>Demonstrate</span></a></li>
 
<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Demonstrate"><span>Demonstrate</span></a></li>
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<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Engagement"><span>Engagement</span></a></li>
 
<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Engagement"><span>Engagement</span></a></li>
 
</li>
 
</li>
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<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Communication"><span>Communication</span></a></li>
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<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/IAT"><span>Iat</span></a></li>
 
</li>
 
</li>
 
<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Societal_Considerations"><span>Societal Considerations</span></a></li>
 
<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Societal_Considerations"><span>Societal Considerations</span></a></li>
 
</li>
 
</li>
 
</ul>
 
</ul>
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<li class="dropdown nav-item"><a href="#" class="nav-link dropdown-toggle nav-link" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Awards<span class="caret"></span></a>
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<li class="dropdown nav-item"><a href="#" class="nav-link dropdown-toggle nav-link" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Dry Lab<span class="caret"></span></a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
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<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Model"><span>Model</span></a></li>
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</li>
 
<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Software"><span>Software</span></a></li>
 
<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Software"><span>Software</span></a></li>
 
</li>
 
</li>
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<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Model"><span>Model</span></a></li>
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<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Automation"><span>Automation</span></a></li>
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</li>
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<li class=""><a class="dropdown-item" href="https://2018.igem.org/Team:Waterloo/Turbidostat"><span>Turbidostat</span></a></li>
 
</li>
 
</li>
 
</ul>
 
</ul>
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<div class="content">
 
<div class="content">
 
 
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   <div class="titleBox row">
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     <div class="layer">
 
       <div class="squiggle squiggleForward col-xs-4"></div>
 
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   </div>
 
   </div>
  
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<div class="row"><div class="col"><div class="content-main"><h2 id="empty-jt2">Empty JT2</h2>
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<div class="row"><div class="col"><div class="content-main"><p><img align="left" src="https://static.igem.org/mediawiki/2018/a/a9/T--Waterloo--MeetMicro_JT2CcaSR.png"></p>
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<p><img src="https://static.igem.org/mediawiki/2018/5/56/T--Waterloo--MeetMicro_JT2.png" alt="Empty JT2"></p>
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<p><strong>Strain</strong>: <em>Escherichia coli</em> <a href="https://www.addgene.org/80403/">JT2 SKA974</a>. This strain has been engineered for optogenetic control using CcaS/R. Its genomic MetE gene is under control of the CcaS/R promoter. It also has a Kanamycin resistance gene in its genome. </p>
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<h4 id="strain">Strain</h4>
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<p><strong>Plasmids</strong>:</p>
−
<ul>
+
−
<li><p><em>Escherichia coli</em> <a href="https://www.addgene.org/80403/">JT2 SKA974</a> </p>
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−
</li>
+
−
<li><p>This strain has been engineered for optogenetic control using CcaS/R. Its genomic MetE gene is under control of the CcaS/R promoter. It also has a Kanamycin resistance gene in its genome. </p>
+
−
</li>
+
−
</ul>
+
−
<h4 id="plasmids">Plasmids</h4>
+
−
<ul>
+
−
<li>None</li>
+
−
</ul>
+
−
<h4 id="antibiotic-resistance-s-">Antibiotic Resistance(s)</h4>
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−
<ul>
+
−
<li>Kanamycin</li>
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−
</ul>
+
−
<h4 id="growth-rate">Growth Rate</h4>
+
−
<ul>
+
−
<li><h4 id="fluorescent-marker">Fluorescent Marker</h4>
+
−
</li>
+
−
<li>None</li>
+
−
</ul>
+
−
<h4 id="can-produce-methionine-">Can produce methionine?</h4>
+
−
<ul>
+
−
<li>No </li>
+
−
</ul>
+
−
<h4 id="which-experiments-was-i-involved-in-">Which experiments was I involved in?</h4>
+
−
<ul>
+
−
<li>Is methionine shared between cells? </li>
+
−
<li>Biobrick <a href="http://parts.igem.org/Part:BBa_K2573000">BBa_K2573000 characterization</a> </li>
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−
</ul>
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−
<h2 id="jt2-ccas-r">JT2 - CcaS/R</h2>
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−
<p><img src="https://static.igem.org/mediawiki/2018/a/a9/T--Waterloo--MeetMicro_JT2CcaSR.png" alt="JT2 CcaS/R"> </p>
+
−
<h4 id="strain">Strain</h4>
+
−
<ul>
+
−
<li><em>Escherichia coli</em> <a href="https://www.addgene.org/80403/">JT2 SKA974</a>
+
−
This strain has been engineered for optogenetic control using CcaS/R. Its genomic MetE gene is under control of the CcaS/R promoter. It also has a Kanamycin resistance gene in its genome. </li>
+
−
</ul>
+
−
<h4 id="plasmids">Plasmids</h4>
+
 
<ul>
 
<ul>
 
<li><a href="https://www.addgene.org/63197/">pSR43.6r</a> (CcaS, its photoreceptor, p15A origin of replication)</li>
 
<li><a href="https://www.addgene.org/63197/">pSR43.6r</a> (CcaS, its photoreceptor, p15A origin of replication)</li>
 
<li><a href="https://www.addgene.org/63176/">pSR58.6</a> (CcaR, sfGFP under the CcaR promoter, ColeI origin of replication)<ul>
 
<li><a href="https://www.addgene.org/63176/">pSR58.6</a> (CcaR, sfGFP under the CcaR promoter, ColeI origin of replication)<ul>
−
<li>Note that we made 2 versions of this strain. One contains both these plasmids, unaltered, and was used for characterization experiments. We cut GFP out of pSR58.6 for the second version of this strain, and used it for non-characterization experiments.  </li>
+
<li>Note that we made two versions of this strain. One contains both these plasmids, unaltered, and was used for characterization  
 +
experiments. We cut GFP out of pSR58.6 for the second version of this strain, and used it for non-characterization experiments.  </li>
 
</ul>
 
</ul>
 
</li>
 
</li>
 
</ul>
 
</ul>
−
<h4 id="antibiotic-resistance-s-">Antibiotic Resistance(s)</h4>
+
<p><strong>Antibiotic Resistance(s)</strong>: Kanamycin, Spectinomycin, Chloramphenicol </p>
−
<ul>
+
<p><strong>Fluorescent Marker</strong>: GFP in the characterization version of this strain, only expressed when CcaS/R is activated.</p>
−
<li>Kanamycin</li>
+
<p><strong>Can produce methionine?</strong> Yes, but only when CcaS/R is activated. </p>
−
<li>Spectinomycin</li>
+
<hr>
−
<li>Chloramphenicol </li>
+
<p><img align="left" src="https://static.igem.org/mediawiki/2018/5/56/T--Waterloo--MeetMicro_JT2.png"></p>
−
</ul>
+
<p><strong>Strain</strong>: <em>Escherichia coli</em> <a href="https://www.addgene.org/80403/">JT2 SKA974</a>. </p>
−
<h4 id="growth-rate">Growth Rate</h4>
+
<p>This strain has been engineered for optogenetic control using CcaS/R. </p>
−
<ul>
+
<p>Its genomic MetE gene is under control of the CcaS/R promoter. </p>
−
<li><h4 id="fluorescent-marker">Fluorescent Marker</h4>
+
<p>It also has a Kanamycin resistance gene in its genome. </p>
−
</li>
+
<p><strong>Plasmids</strong>: None</p>
−
<li>GFP in the characterization version of this strain, only expressed when CcaS/R is activated.</li>
+
<p><strong>Antibiotic Resistance(s)</strong>: Kanamycin</p>
−
</ul>
+
<p><strong>Fluorescent Marker</strong>: None</p>
−
<h4 id="can-produce-methionine-">Can produce methionine?</h4>
+
<p><strong>Can produce methionine?</strong> No </p>
−
<ul>
+
<hr>
−
<li>Yes, but only when CcaS/R is activated. </li>
+
<p><img align="left" src="https://static.igem.org/mediawiki/2018/c/c9/T--Waterloo--MeetMicro_GFP.png"></p>
−
</ul>
+
<p><strong>Strain</strong>: <em>Escherichia coli</em> DH5alpha </p>
−
<h4 id="which-experiments-was-i-involved-in-">Which experiments was I involved in?</h4>
+
<p><strong>Plasmids</strong>:</p>
−
<ul>
+
−
<li>Is methionine shared between cells? </li>
+
−
<li>What is the metabolic load of (over)expressing MetE?</li>
+
−
<li>Can we control growth with red and green light? </li>
+
−
</ul>
+
−
<h2 id="empty-dh5alpha">Empty DH5alpha</h2>
+
−
<p><img src="https://static.igem.org/mediawiki/2018/9/92/T--Waterloo--MeetMicro_dh5.png" alt="Empty DH5"> </p>
+
−
<h4 id="strain">Strain</h4>
+
−
<ul>
+
−
<li><em>Escherichia coli</em> DH5alpha </li>
+
−
</ul>
+
−
<h4 id="plasmids">Plasmids</h4>
+
−
<ul>
+
−
<li>None</li>
+
−
</ul>
+
−
<h4 id="antibiotic-resistance-s-">Antibiotic Resistance(s)</h4>
+
−
<ul>
+
−
<li>None</li>
+
−
</ul>
+
−
<h4 id="growth-rate">Growth Rate</h4>
+
−
<ul>
+
−
<li><h4 id="fluorescent-marker">Fluorescent Marker</h4>
+
−
</li>
+
−
<li>None</li>
+
−
</ul>
+
−
<h4 id="can-produce-methionine-">Can produce methionine?</h4>
+
−
<ul>
+
−
<li>Yes, with my wild type genomic MetE gene. </li>
+
−
</ul>
+
−
<h3 id="which-experiments-was-i-involved-in-">Which experiments was I involved in?</h3>
+
−
<ul>
+
−
<li>Can we reliably measure 2 populations in co-culture?</li>
+
−
</ul>
+
−
<h2 id="dh5alpha-gfp">DH5alpha - GFP</h2>
+
−
<p><img src="" alt="DH5 GFP"></p>
+
−
<h3 id="strain">Strain</h3>
+
−
<ul>
+
−
<li><em>Escherichia coli</em> DH5alpha</li>
+
−
</ul>
+
−
<h3 id="plasmids">Plasmids</h3>
+
 
<ul>
 
<ul>
 
<li><a href="http://parts.igem.org/Part:BBa_E0040">BBa_E0040 in pSB1C3</a> (GFP biobrick, ColeI origin of replication)</li>
 
<li><a href="http://parts.igem.org/Part:BBa_E0040">BBa_E0040 in pSB1C3</a> (GFP biobrick, ColeI origin of replication)</li>
 
<li>a plasmid we constructed that has Spectinomycin resistance and p15A origin of replication</li>
 
<li>a plasmid we constructed that has Spectinomycin resistance and p15A origin of replication</li>
 
</ul>
 
</ul>
−
<h3 id="antibiotic-resistance-s-">Antibiotic Resistance(s)</h3>
+
<p><strong>Antibiotic Resistance(s)</strong>: Spectinomycin, Chloramphenicol </p>
−
<ul>
+
<p><strong>Fluorescent Marker</strong>: GFP, constitutively expressed. </p>
−
<li>Spectinomycin</li>
+
<p><strong>Can produce methionine?</strong>: Yes, with wild type genomic MetE gene. </p>
−
<li>Chloramphenicol </li>
+
<hr>
−
</ul>
+
<p><img align="left" src="https://static.igem.org/mediawiki/2018/9/92/T--Waterloo--MeetMicro_dh5.png"></p>
−
<h3 id="growth-rate">Growth Rate</h3>
+
<p><strong>Strain</strong>: <em>Escherichia coli</em> DH5alpha</p>
−
<ul>
+
<p><strong>Plasmids</strong>: None</p>
−
<li><h3 id="fluorescent-marker">Fluorescent Marker</h3>
+
<p><strong>Antibiotic Resistance(s)</strong> None</p>
−
</li>
+
<p><strong>Fluorescent Marker</strong> None</p>
−
<li>GFP, constitutively expressed. </li>
+
<p><strong>Can produce methionine?</strong> Yes, with wild type genomic MetE gene. </p>
−
</ul>
+
−
<h3 id="can-produce-methionine-">Can produce methionine?</h3>
+
−
<ul>
+
−
<li>Yes, with my wild type genomic MetE gene. </li>
+
−
</ul>
+
−
<h3 id="which-experiments-was-i-involved-in-">Which experiments was I involved in?</h3>
+
−
<ul>
+
−
<li>Can we reliably measure 2 populations in co-culture?</li>
+
−
</ul>
+
 
</div></div></div>
 
</div></div></div>
 
</div>
 
</div>
 
</html>
 
</html>
 
{{Waterloo/footer}}
 
{{Waterloo/footer}}

Latest revision as of 05:09, 1 December 2018

Meet the Microbes

Strain: Escherichia coli JT2 SKA974. This strain has been engineered for optogenetic control using CcaS/R. Its genomic MetE gene is under control of the CcaS/R promoter. It also has a Kanamycin resistance gene in its genome.

Plasmids:

  • pSR43.6r (CcaS, its photoreceptor, p15A origin of replication)
  • pSR58.6 (CcaR, sfGFP under the CcaR promoter, ColeI origin of replication)
    • Note that we made two versions of this strain. One contains both these plasmids, unaltered, and was used for characterization experiments. We cut GFP out of pSR58.6 for the second version of this strain, and used it for non-characterization experiments.

Antibiotic Resistance(s): Kanamycin, Spectinomycin, Chloramphenicol

Fluorescent Marker: GFP in the characterization version of this strain, only expressed when CcaS/R is activated.

Can produce methionine? Yes, but only when CcaS/R is activated.

Strain: Escherichia coli JT2 SKA974.

This strain has been engineered for optogenetic control using CcaS/R.

Its genomic MetE gene is under control of the CcaS/R promoter.

It also has a Kanamycin resistance gene in its genome.

Plasmids: None

Antibiotic Resistance(s): Kanamycin

Fluorescent Marker: None

Can produce methionine? No

Strain: Escherichia coli DH5alpha

Plasmids:

  • BBa_E0040 in pSB1C3 (GFP biobrick, ColeI origin of replication)
  • a plasmid we constructed that has Spectinomycin resistance and p15A origin of replication

Antibiotic Resistance(s): Spectinomycin, Chloramphenicol

Fluorescent Marker: GFP, constitutively expressed.

Can produce methionine?: Yes, with wild type genomic MetE gene.

Strain: Escherichia coli DH5alpha

Plasmids: None

Antibiotic Resistance(s) None

Fluorescent Marker None

Can produce methionine? Yes, with wild type genomic MetE gene.

Waterloo iGEM 2018

© 2018 Waterloo iGEM