Difference between revisions of "Team:NUDT CHINA/InterLab"

Line 1: Line 1:
−
{{NUDT_CHINA}}
+
{{NUDT_CHINA/navbar}}
 
<html>
 
<html>
 +
<style>
 +
#content {
 +
    padding: 0px;
 +
    margin: 0px;
 +
    width: 100%;
 +
}
 +
body {
 +
    background-color: white;
 +
    margin: 0px;
 +
}
 +
#bodyContent h1 {
 +
    font-weight: 300 !important;
 +
}
 +
#bodyContent h1,
 +
#bodyContent h2,
 +
#bodyContent h3,
 +
#bodyContent h4,
 +
#bodyContent h5 {
 +
    margin-bottom: 0px;
 +
    border-bottom: none;
 +
}
  
 +
#bodyContent {
 +
    padding-right: 0px;
 +
}
 +
#globalWrapper {
 +
    font-size: 100%;
 +
    padding: 0px;
 +
    margin: 0px;
 +
}
 +
#top_title {
 +
    visibility: hidden;
 +
    display:none;
 +
}
 +
/* Sections Header
 +
--------------------------------*/
 +
.section-header h3 {
 +
  font-size: 40px;
 +
  color: #111;
 +
  text-transform: uppercase;
 +
  text-align: center;
 +
  font-weight: 700;
 +
  position: relative;
 +
  padding-bottom: 15px;
 +
}
  
−
<div class="column full_size judges-will-not-evaluate">
+
.section-header h3::before {
−
<h3>Introduction </h3>
+
  content: '';
−
<p>The InterLab this year has been updated to a more detailed protocol. During the InterLab study, we used the LUDOX, fluorescence and the plasmids that were shipped along with the distribution kit, and closely followed the InterLab study protocol.  </p>
+
  position: absolute;
−
<p> Through the InterLab 2018 study, we experienced a lot in the measuring work, and surprisingly got to know more about the instruments that usually were ignored by us. Actually, it is the measuring instruments that help us complete the project every year!! </p>
+
  display: block;
−
</div>
+
  width: 120px;
 +
  height: 1px;
 +
  background: #ddd;
 +
  bottom: 1px;
 +
  left: calc(50% - 60px);
 +
}
  
 +
.section-header h3::after {
 +
  content: '';
 +
  position: absolute;
 +
  display: block;
 +
  width: 40px;
 +
  height: 3px;
 +
  background: #18d26e;
 +
  bottom: 0;
 +
  left: calc(50% - 20px);
 +
}
  
−
<div class="clear"></div>
+
.section-header p {
 +
  text-align: center;
 +
  padding-bottom: 30px;
 +
  color: #333;
 +
}
  
 +
/* Back to top button */
 +
.back-to-top {
 +
  position: fixed;
 +
  background: #18d26e;
 +
  background-repeat: no-repeat;
 +
  background-size: contain;
 +
  color: #fff;
 +
  display: inline-block;
 +
  width: 60px;
 +
  height: 60px;
 +
  text-align: center;
 +
  line-height: 1;
 +
  font-size: 16px;
 +
  border-radius: 50%;
 +
  right: 30px;
 +
  bottom: 30px;
 +
  transition: background 0.5s;
 +
  z-index: 500;
 +
}
  
−
<div class="column full_size">
+
.back-to-top i {
−
<h1>Methods and Design</h1>
+
  padding-top: 12px;
−
<h3>1. InterLab Parts</h3>
+
  color: #fff;
−
<p>    Positive Control (BBa_I20270)
+
}
−
</p>
+
−
<p>    NegativeControl (BBa_R0040)
+
−
</p>
+
−
<p>    Test Device1 (BBa_J364000)
+
−
</p>
+
−
<p>    Test Device2 (BBa_J364001)
+
−
</p>
+
−
<p>    Test Device3 (BBa_J364002)
+
−
</p>
+
−
<p>    Test Device4 (BBa_J364007)
+
−
</p>
+
−
<p>    Test Device5 (BBa_J364008)   
+
−
</p>                                                   
+
−
<p>    Test Device6 (BBa_J364009)
+
−
 
+
−
</p>
+
−
</div>
+
−
 
+
−
<div class="column full_size">
+
−
<h3>2.  Preparation</h3>
+
−
<p>    To start with, our team transformed E.coli strain DH5α with the provided plasmids, namely Test Device 1,2,3,4,5,6, Positive Control, and Negative Control. As long as colonies had emerged on Cm+ Resistance Media, we picked up 2 colonies in each petrie dish and cultured them at 37℃ with 220 rpm frequency for 14 hours. When the bacteria solutions were turbid enough, we began the following process.
+
−
 
+
−
</p>
+
−
</div>
+
−
 
+
−
<div class="column full_size">
+
−
<h3>3.  OD600 Reference Point</h3>
+
−
<p>    With plate reader, we measured Abs600 of the LUDOX and H2O. The H2O measurement served as the background. Both included 4 technical replicates to enhance the reliability of the results. Comparing to the standard OD600 reference given, which was 0.0425, we were able to achieve a ratio between OD600 and Abs600. The ratio was essential in converting Abs600 raw measurements into standard OD600 records.
+
−
 
+
−
</p>
+
−
</div>
+
−
 
+
−
<div class="column full_size">
+
−
<h3>4. Fluorescein Standard Curve</h3>
+
−
<p>    Different concentrations of fluorescein were obtained by 2-fold serial dilution. In the first pipet, 200μL Fluorescein 1×stock solution was added. By removing half of the 200μL solution in previous pipets to later ones which already contained 100μL PBS, we were able to generate 11 solutions in half-descending fluorescence concentration. We also included another pipet with 100μL PBS only as blank. Within each concentration, we performed 4 replicates to calculate the more representative mean. After the results were recorded, all fluorescein concentrations were divided by average fluorescence measurement, of which the medium-high mean were calculated to diminish operation errors. This mean point would be used to set up the conversion between fluorescein concentration and fluorescence measurements in later steps.
+
−
 
+
−
</p>
+
−
</div>
+
−
 
+
−
<div class="column full_size">
+
−
<h3>5. OD600 and Fluorescence Measurements</h3>
+
−
<p>    We tracked the original OD600 of the 16 samples so as to dilute them into 0.02. Once the original values were available, we integrated them into the dilution calculation sheet and followed the suggested volume to dilute each sample. After dilution, we confirmed that OD600 had reached exactly 0.02 or around. Our team set that time as T=0(h) and measured average OD600 from 4 replicates of each 16 samples to reduce technical error. Other 4 replicates were used for T=0(h) fluorescence measurements, with the same equipment and settings in Step 3. Henceforward, we recorded the OD600 and fluorescence when T=0 and T=6.
+
−
 
+
−
</p>
+
−
</div>
+
−
 
+
−
<div class="column full_size">
+
−
<h3>6. Equipments and Settings</h3>
+
−
<p>    To obtain OD600 measurement, we employed Multiscan FC. Single wavelength of 600nm was used, and path length correction was turned off. To reduce measurement error, we also added a dynamic circulation of 5 times, with 2-second intervals.
+
−
 
+
−
</p>
+
−
<p>    The fluorescence measurement was obtained through Fluoroskan Ascent FL and Thermo Fisher. The filters used were 485nm and 538nm for excitation and emission respectively. The measurement cycled 5 times with intervals of 2 second.
+
−
 
+
−
</p>
+
−
</div>
+
  
−
<div class="column full_size">
+
@media (max-width: 768px) {
−
<h1>Results & Discussion</h1>
+
  .back-to-top {
−
<h3>Calibration</h3>
+
    bottom: 30px;
−
<p>   Calibration1: OD600 Reference point – LUDOX Protocol
+
  }
 +
}
 +
</style>
 +
<div class="col-md-12 column" alt="cover_photo" style="width:100%;height:250px;background-size:cover;background-position:0px -350px; background-image:url(https://static.igem.org/mediawiki/2016/thumb/3/3f/NUDT_CHINA2016_FILES-img-banner-3.0.png/800px-NUDT_CHINA2016_FILES-img-banner-3.0.png);background-repeat:no-repeat;">
 +
  <p style="font-size:36px;margin: 0 0%;padding: 8% 0 0 10%;color: white;">Development of A Novel</p>
 +
<p style="font-size:36px;margin: 0 0%;padding: 0 0 0 10%;color: white;">Blood-MicroRNA Handy Detection System with CRISPR</p></div>
  
−
</p>
+
<div class="container">
−
<p>   We used LUDOX CL-X (45% colloidal silica suspension) , ddH2O and 96 well plate(black with clear flat bottom preferred) to get the conversion factor, which can transform Abs600 measurements into comparable OD600 measurements.
+
<div class="row clearfix">
 +
<div class="container" style="margin-top: 280px">
 +
<div class="row" style="margin-top: 25px">
 +
                                <div class="col-md-push-1 col-md-10">
 +
      <h1 style="font-size: 40px;font-weight: 900">Our Work</h1>
 +
                                      <h2 style="font-size: 32px;margin-bottom: 10px;margin-top: 15px;">Our Work</h2>
 +
<p alt="content_add" style="font-size: 18px;">hello everyone this is the another example-content part for more imformation. Hello everyone this is the another example-content part for more imformation.Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui. </p>
 +
<p alt="content_add" style="font-size: 18px;">
 +
Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.
 +
</p>
 +
</div>
 +
<div class="col-md-12 column" alt = "main_content">
 +
<div class="col-md-8 col-md-push-2 thumbnail" alt="content_pictute">
 +
<img alt="300x200" src="https://static.igem.org/mediawiki/2018/9/9b/2018_igem_menu_logo.svg" />
 +
<p alt="photo_detail" style="text-align: center;">This is the sign of the famous game!</p>
 +
</div>
 +
 +
<div class="col-md-6 col-md-push-3">
 +
<!-- <p class="text-center text-info">this is the sign of igem</p> -->
 +
<p alt="photo_detail" style="text-align: center;"><em>This is the sign of the famous game!</em></p>
 +
</div>
 +
</div>
 +
<div class="col-md-push-1 col-md-10">
 +
<p alt="content_add" style="font-size: 18px;">hello everyone this is the another example-content part for more imformation. Hello everyone this is the another example-content part for more imformation.Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui. </p>
 +
<p alt="content_add" style="font-size: 18px;">
 +
Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.
 +
</p>
 +
<blockquote style="border-left-color: #18d26e;">
 +
Maybe you want to write <strong>something interesting thing</strong> here!
 +
<small>Someone famous in <cite>Source Title</cite></small>
 +
</blockquote>
 +
<p alt="content_add" style="font-size: 18px;">hello everyone this is the another example-content part for more imformation. Hello everyone this is the another example-content part for more imformation.Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui. </p>
 +
<p alt="content_add" style="font-size: 18px;">
 +
Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.
 +
</p>
 +
</div>
 +
<div class="col-md-12 column" alt = "main_content">
 +
<div class="col-md-6 col-md-push-3" alt="content_pictute">
 +
<div class="row center-block">
 +
<img alt="300x200" src="https://static.igem.org/mediawiki/2018/9/9b/2018_igem_menu_logo.svg" />
 +
<p alt="photo_detail" style="text-align: center;">This is the sign of the famous game!</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="col-md-push-1 col-md-10">
 +
<p alt="content_add" style="font-size: 18px;">hello everyone this is the another example-content part for more imformation. Hello everyone this is the another example-content part for more imformation.Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui. </p>
 +
<p alt="content_add" style="font-size: 18px;">
 +
Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.
 +
</p>
 +
<table class="table table-striped">
 +
  <caption>The example table for show some data</caption>
 +
  <thead>
 +
<tr>
 +
  <th>name</th>
 +
  <th>city</th>
 +
  <th>mail</th>
 +
</tr>
 +
  </thead>
 +
  <tbody>
 +
<tr>
 +
  <td>Tanmay</td>
 +
  <td>Bangalore</td>
 +
  <td>560001</td>
 +
</tr>
 +
<tr>
 +
  <td>Sachin</td>
 +
  <td>Mumbai</td>
 +
  <td>400003</td>
 +
</tr>
 +
<tr>
 +
  <td>Uma</td>
 +
  <td>Pune</td>
 +
  <td>411027</td>
 +
</tr>
 +
  </tbody>
 +
</table>
 +
<p alt="content_add" style="font-size: 18px;">
 +
Example block-level help text here.There are several variants of  derived from different organisms. In our project, we  . The structure of this protein has been determined with and without  The  a  lobe. The two lobes are positively charged towards the protein core to accommodate the negatively charged RNA. Each of these two lobes contains an RNase domain. At the  main is responsible for target cleavage. In contrast to other  two RNase domains of the NUC lobe are located at the outside of the protein, which is likely the reason collateral cleavage upon activation by binding to a matching target. These two domains have been labeled as red spots in Figure 2 and can be found at the interface between the green and pink domain.
 +
</p>
 +
<p alt="content_add" style="font-size: 18px;">
 +
Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.
 +
</p>
  
−
</p>
+
<div class="col-md-push-1 col-md-4 thumbnail">
 +
<img alt="300x200" src="https://static.igem.org/mediawiki/2018/9/9b/2018_igem_menu_logo.svg" />
 +
</div>
 +
<div class="col-md-push-3 col-md-4 thumbnail">
 +
<img alt="300x200" src="https://static.igem.org/mediawiki/2018/9/9b/2018_igem_menu_logo.svg" />
 +
</div>
 +
</div>
 +
<div class="col-md-push-1 col-md-10">
 +
<p alt="content_add" style="font-size: 18px;">
 +
Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.
 +
</p>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<header class="section-header">
 +
<h3>More details</h3>
 +
</header>
 +
<div class="col-md-12 column" alt="problem_answer">
 +
<div class="panel-group" id="panel-1">
 +
<div class="panel panel-success">
 +
<div class="panel-heading text-center">
 +
<a class="panel-title collapsed " data-toggle="collapse" data-parent="#panel-1" href="#panel-element-1"  style="color: #079209; font-size: 20px;text-decoration:none;">Question 1: What does the structure of domains look like?</a>
 +
</div>
 +
<div id="panel-element-1" class="panel-collapse collapse">
 +
<div class="panel-body" style="font-size:16px;">
 +
There are several variants of  derived from different organisms. In our project, we  . The structure of this protein has been determined with and without  The  a  lobe. The two lobes are positively charged towards the protein core to accommodate the negatively charged RNA. Each of these two lobes contains an RNase domain. At the  main is responsible for target cleavage. In contrast to other  two RNase domains of the NUC lobe are located at the outside of the protein, which is likely the reason collateral cleavage upon activation by binding to a matching target. These two domains have been labeled as red spots in Figure 2 and can be found at the interface between the green and pink domain.
 +
</div>
 +
</div>
 +
</div>
 +
<div class="panel panel-success">
 +
<div class="panel-heading text-center">
 +
<a class="panel-title collapsed" data-toggle="collapse" data-parent="#panel-1" href="#panel-element-2" style="color: #079209; font-size: 20px;text-decoration:none;">Collapsible Group Item #2</a>
 +
</div>
 +
<div id="panel-element-2" class="panel-collapse collapse">
 +
<div class="panel-body" style="font-size:16px;">
 +
Anim pariatur cliche...
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 
</div>
 
</div>
  
  
 
</html>
 
</html>
 +
{{NUDT_CHINA/footer}}

Revision as of 01:06, 10 August 2018

Development of A Novel

Blood-MicroRNA Handy Detection System with CRISPR

Our Work

Our Work

hello everyone this is the another example-content part for more imformation. Hello everyone this is the another example-content part for more imformation.Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.

Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.

300x200

This is the sign of the famous game!

This is the sign of the famous game!

hello everyone this is the another example-content part for more imformation. Hello everyone this is the another example-content part for more imformation.Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.

Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.

Maybe you want to write something interesting thing here! Someone famous in Source Title

hello everyone this is the another example-content part for more imformation. Hello everyone this is the another example-content part for more imformation.Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.

Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.

300x200

This is the sign of the famous game!

hello everyone this is the another example-content part for more imformation. Hello everyone this is the another example-content part for more imformation.Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.

Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.

The example table for show some data
name city mail
Tanmay Bangalore 560001
Sachin Mumbai 400003
Uma Pune 411027

Example block-level help text here.There are several variants of derived from different organisms. In our project, we . The structure of this protein has been determined with and without The a lobe. The two lobes are positively charged towards the protein core to accommodate the negatively charged RNA. Each of these two lobes contains an RNase domain. At the main is responsible for target cleavage. In contrast to other two RNase domains of the NUC lobe are located at the outside of the protein, which is likely the reason collateral cleavage upon activation by binding to a matching target. These two domains have been labeled as red spots in Figure 2 and can be found at the interface between the green and pink domain.

Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.

300x200
300x200

Donec id elit non mi porta gravida at eget metus. Fusce dapibus, tellus ac cursus commodo, tortor mauris condimentum nibh, ut fermentum massa justo sit amet risus. Etiam porta sem malesuada magna mollis euismod. Donec sed odio dui.

More details

There are several variants of derived from different organisms. In our project, we . The structure of this protein has been determined with and without The a lobe. The two lobes are positively charged towards the protein core to accommodate the negatively charged RNA. Each of these two lobes contains an RNase domain. At the main is responsible for target cleavage. In contrast to other two RNase domains of the NUC lobe are located at the outside of the protein, which is likely the reason collateral cleavage upon activation by binding to a matching target. These two domains have been labeled as red spots in Figure 2 and can be found at the interface between the green and pink domain.
Anim pariatur cliche...