Difference between revisions of "Team:Pasteur Paris/ExperimentsExperiment"

Line 983: Line 983:
  
 
                     <div class="vignette_text">
 
                     <div class="vignette_text">
−
                         <p style="margin:auto; text-align:center;font-weight:bold;">PDMS Chips</p>
+
                         <p style="margin:auto; text-align:center;font-weight:bold;" >PDMS Chips</p>
 
                     </div>
 
                     </div>
 
                 </div>
 
                 </div>

Revision as of 08:58, 10 August 2018

Training

PROTOCOLS

Microfluidics

PDMS (Polydimethylsiloxane) is a widely used polymer in microfluidics, for its biocompatibility and transparence, among other qualities. Here we show how to prepare PDMS for microfluidic chips, as well as how to demold them, bond them to other surfaces and treat them for neuron growth.

PDMS Chips


Materials

  • Sylgard 184 Elastomer Kit (Sigma-aldrich, Sylgard 184, 761036-5EA)
  • Razor blade (OEMTOOLS 25181 Razor Blades, 100 Pack)
  • 4mm Biopsy Puncher (Kai Biopsy Punch 4mm )
  • Isopropanol (Fisherbrand, CAS number 67-63-0)
  • Vacuum pump unit (Vacuubrand PC 3 RZ 2.5)

Protocol

According to manufacturer's instruction.

  • Mix monomer and curing agent (10:1 proportion) for 30 seconds
  • Use a vacuum machine to extract air bubbles until the mixture is clear
  • Pour mixture onto mold
  • Put mixture+mold in stove at 70 degrees Celsius for 3 hours
  • Use razor blade to cut the borders of the chip and extract the chip from its mold
  • Use biopsy puncher to drill input and output holes
  • Clean chip with isopropanol

Get full protocol here